Results 61 to 70 of about 1,110,843 (197)

Cryo-electron microscopy reveals human low density lipoprotein substructure.

Journal of Lipid Research, 1994
In the present study, we have examined the structure of human low density lipoprotein (LDL) using cryo-electron microscopy. Human LDL particles were analyzed in a vitrified frozen-hydrated condition, without chemical fixation or any form of staining ...
R Van Antwerpen, J C Gilkey
doaj   +1 more source

mmCIF Validator: a comprehensive validation tool for structural biology data files

Journal of Applied Crystallography, Volume 59, Issue 4, Page 1338-1343, August 2026.
The mmCIF Validator provides real‐time automated validation of mmCIFs against a valid PDBx/mmCIF dictionary. The validator is available as both a Visual Studio Code extension and a standalone Python script for integration into automated workflows.The PDBx/macromolecular crystallographic information file (PDBx/mmCIF) framework is the standard for ...
Deborah Harrus, Genevieve L. Evans, Sreenath Sasidharan Nair, Weslley Morellato Bueno, Jennifer Fleming, Sameer Velankar   +5 more
wiley   +1 more source

Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation

, 2011
mRNA–tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion.
Jie Fu, Scott C. Blanchard, Joachim Frank, James B. Munro   +3 more
core   +1 more source

Structural studies of tropomyosin by cryoelectron microscopy and x-ray diffraction [PDF]

, 1991
A comparison has been made between cryoelectron microscope images and the x-ray structure of one projection of the Bailey tropomyosin crystal. The computed transforms of the electron micrographs extend to a resolution of approximately 18 A compared with ...
Sosinsky, G.E., Melanson, L., Cohen, C., Cabral-Lilly, D., Chacko, S., Phillips, G.N.   +5 more
core   +1 more source

Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy

Bio-Protocol, 2021
Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function.
Corbin Black, Daniel Dai, Katya Peri, Muneyoshi Ichikawa, Khanh Huy Bui   +4 more
doaj   +1 more source

Potent monoclonal antibodies against multidrug‐resistant hypervirulent Klebsiella pneumoniae

Interdisciplinary Medicine, Volume 4, Issue 4, July 2026.
A novel immunization strategy using a low‐virulence, multidrug‐resistant strain yields synergistic monoclonal antibodies against hypervirulent Klebsiella pneumoniae. These antibodies provide cross‐serotype protection through a dual‐mechanism of pathogen clearance and immunomodulation, offering a promising non‐antibiotic therapeutic for resistant ...
Yushan Jiang, Zhuolin Li, Yang Yang, Zihan He, Tyuji Hoshino, Vladislav Victorovich Khrustalev, Qing Zhu, Weiling Wu, Wei Zhao, Chenguang Shen, Shi Ouyang   +10 more
wiley   +1 more source

Automated cryoelectron microscopy of “single particles” applied to the 26S proteasome [PDF]

FEBS Letters, 2007
The 26S proteasome is a large molecular machine with a central role in intracellular protein degradation in eukaryotes. The 2.5 MDa complex, which is built from two copies each of more than 30 different subunits, is labile and prone to dissociation into subcomplexes.
Nickell, S., Beck, F., Korinek, A., Mihalache, O., Baumeister, W., Plitzko, J.   +5 more
openaire   +3 more sources

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]

, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Vettenburg, T.; id_orcid, J. Nylk, K. Dholakia, Nylk, J., C. Mitchell, Vettenburg, T., Gunn-Moore, F. J., T. Vettenburg, Mitchell, C., Dholakia, K., Nylk, J.; id_orcid, F. J. Gunn-Moore   +11 more
core   +1 more source

Mapping the Conformational Landscape of DNA Minicircles Through Atomic Force Microscopy and Shape Space Analysis

Small, Volume 22, Issue 37, 2 July 2026.
An automated framework is presented for quantifying DNA conformational variability from atomic force microscopy (AFM) images. DNA backbones are reduced to spline representations and analyzed using cyclic Procrustes analysis to measure shape similarity across ensembles.
Laura Wiggins, Tobias A. Firth, Max C. Gamill, Thomas E. Catley, Agnes Noy, Jonathan M. Fogg, Lynn Zechiedrich, Alice L. B. Pyne   +7 more
wiley   +1 more source

Using cryo-electron microscopy maps for X-ray structure determination

IUCrJ, 2018
X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized ...
Lingxiao Zeng, Wei Ding, Quan Hao
doaj   +1 more source