Results 61 to 70 of about 17,043 (166)
Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
Bio-Protocol, 2021 Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function.
Corbin Black +4 more
doaj +1 more source
Advances in GPCRs Associated With Wnt Signaling Within the Auditory System
Medicine Bulletin, Volume 2, Issue 3, Page 280-298, June 2026.ABSTRACT G protein‐coupled receptors (GPCRs) that interact with the Wnt signaling pathway are pivotal for auditory system homeostasis, as they orchestrate inner ear development, hair cell (HC) regeneration, and hearing preservation. Frizzled (FZD) receptors, the core Wnt‐related GPCRs, bind Wnt ligands and co‐receptors (e.g., LRP5/6) to activate both ...
Liang Wang +3 more
wiley +1 more source
Using cryo-electron microscopy maps for X-ray structure determination
IUCrJ, 2018 X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized ...
Lingxiao Zeng, Wei Ding, Quan Hao
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Integrative structural modelling and visualisation of a cellular organelle
QRB Discovery, 2022 Models of insulin secretory vesicles from pancreatic beta cells have been created using the cellPACK suite of tools to research, curate, construct and visualise the current state of knowledge. The model integrates experimental information from proteomics,
Ludovic Autin +6 more
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Acta Crystallographica Section D, Volume 82, Issue 6, Page 638-645, June 2026.We present a quantitative framework combining cost modelling and queueing theory to estimate hourly access costs, waiting times and congestion levels for shared high‐end scientific equipment. The approach is general, illustrated with cryo‐electron microscopy examples, and implemented in an open‐access online calculator to support evidence‐based ...
C. O. S. Sorzano, J. M. Carazo
wiley +1 more source
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes
Computational and Structural Biotechnology Journal, 2016 Cryo-electron microscopy (cryo-EM) has for a long time been a technique of choice for determining structure of large and flexible macromolecular complexes that were difficult to study by other experimental techniques such as X-ray crystallography or ...
Slavica Jonić
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Cryo‐EM structure of ALC1 in an open conformation bound to a PARylated nucleosome
Acta Crystallographica Section D, Volume 82, Issue 6, Page 683-699, June 2026.Reanalysis of a publicly available cryo‐EM dataset identifies a new conformation of the oncogenic chromatin remodeler ALC1/CHD1L bound to a PARylated nucleosome. This new structure reveals the position of the macro domain in the complex, and may help to understand the large conformational change leading to the activation of ALC1.Nucleosomes are the ...
Hannah R. Bridges +3 more
wiley +1 more source
Acta Crystallographica Section D, Volume 82, Issue 6, Page 587-602, June 2026.This review describes how an integrated structural biology platform at Merck Sharp & Dohme LLC, Rahway, New Jersey, USA, combining X‐ray crystallography, cryo‐EM, MicroED and cryo‐ET, enables seamless three‐dimensional insights from molecular to cellular length scales.
Jill E. Chrencik +44 more
wiley +1 more source
Cryo-electron microscopy reveals human low density lipoprotein substructure.
Journal of Lipid Research, 1994 In the present study, we have examined the structure of human low density lipoprotein (LDL) using cryo-electron microscopy. Human LDL particles were analyzed in a vitrified frozen-hydrated condition, without chemical fixation or any form of staining ...
R Van Antwerpen, J C Gilkey
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The Plant Journal, Volume 126, Issue 5, June 2026.Significance Statement Euglena gracilis is an early diverging photosynthetic organism with a unique mosaic photosynthetic apparatus. Biochemical and spectroscopic analysis reveals a kinetically slower photosystem I sharing excitation energy with photosystem II in the absence of strict photosystem segregation.
Parveen Akhtar +6 more
wiley +1 more source