Results 71 to 80 of about 22,940 (199)

The evolving role of structural biology in pharma: integration of X‐ray crystallography, cryo‐electron microscopy and beyond

Acta Crystallographica Section D, Volume 82, Issue 6, Page 587-602, June 2026.
This review describes how an integrated structural biology platform at Merck Sharp & Dohme LLC, Rahway, New Jersey, USA, combining X‐ray crystallography, cryo‐EM, MicroED and cryo‐ET, enables seamless three‐dimensional insights from molecular to cellular length scales.
Jill E. Chrencik, Hua-Poo Su, Yacob Gomez Llorente, Rachel L. Palte, Daniel J. Klein, Robert P. Hayes, Sadie P. Antine, Guilermo A. Asmar-Rovira, Nicole Bertoletti, Noel J. Byrne, Brittany L. Carroll, Ciro D. Cordeiro, Kaylynn Curfman, James Dutko, Michael J. Eddins, Thierry Fischmann, Barani Govindarajan, Corey W. Hecksel, Thu Ho, Kaspar Hollenstein, Mee Ra Hong, Alan Hruza, Andrii Ishchenko, Maru Jaime-Garza, Matt J. Jaremko, Hayley L. Knox, James Kostas, Harini Krishnamurthy, Ioannis Manolaridis, Cameron L. Noland, Andrea T. Partridge, Sangita B. Patel, Paul Reichert, John C. Reid, Catarina Felisberto-Rodrigues, Chitra A. Shintre, Jennifer M. Shipman, Elakkiya Tamilselvan, Morgan E. Walker, Susanne N. Walker, Renjing Wang, Katharine M. Wright, Tien-Jui Yen, Byung-Kuk Yoo, Sandra B. Gabelli   +44 more
wiley   +1 more source

Integrative structural modelling and visualisation of a cellular organelle

QRB Discovery, 2022
Models of insulin secretory vesicles from pancreatic beta cells have been created using the cellPACK suite of tools to research, curate, construct and visualise the current state of knowledge. The model integrates experimental information from proteomics,
Ludovic Autin, Brett A. Barbaro, Andrew I. Jewett, Axel Ekman, Shruti Verma, Arthur J. Olson, David S. Goodsell   +6 more
doaj   +1 more source

Dynamic LHCI antenna and inter‐photosystem spillover enable flexible light harvesting in Euglena gracilis

The Plant Journal, Volume 126, Issue 5, June 2026.
Significance Statement Euglena gracilis is an early diverging photosynthetic organism with a unique mosaic photosynthetic apparatus. Biochemical and spectroscopic analysis reveals a kinetically slower photosystem I sharing excitation energy with photosystem II in the absence of strict photosystem segregation.
Parveen Akhtar, László Kovács, Ildikó Domonkos, Ádám Pap, Aladár Pettkó‐Szandtner, Soujanya Kuntam, Petar H. Lambrev   +6 more
wiley   +1 more source

Cryo-electron microscopy structure of a human PRMT5:MEP50 complex.

PLoS ONE, 2018
Protein arginine methyl transferase 5 (PRMT5) is a signaling protein and histone modifying enzyme that is important in many cellular processes, including regulation of eukaryotic gene transcription.
David E Timm, Valorie Bowman, Russell Madsen, Charles Rauch   +3 more
doaj   +1 more source

Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture [PDF]

, 2018
Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the fine structural features of cellular compartments. In the present study, this approach has been applied in order to explore the ultrastructural morphology of ...
Bouchet-Marquis, C., Dubochet, J., Fakan, S.   +2 more
core  

Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography [PDF]

, 2008
Electron tomography is currently the highest resolution imaging modality available to study the 3D structures of pleomorphic macromolecular assemblies, viruses, organelles and cells. Unfortunately, the resolution is currently limited to 3–5 nm by several
Castaño-Díez, Daniel, Fahimian, Benjamin P., Iancu, Cristina V., Jensen, Grant J., Lee, Edwin, Miao, Jianwei, Murphy, Gavin E., Suloway, Christian, Wright, Elizabeth R.   +8 more
core   +1 more source

Histidine Protonation and the Oxidizing Power of the Oxygen‐Evolving Complex

ChemPhotoChem, Volume 10, Issue 5, May 2026.
Protonation of D1‐His337 governs the oxidizing power of the Mn4CaO5 cluster in photosystem II. Its singly protonated form is unstable, resulting in histidine oxidation and disruption of Mn oxidation, whereas the doubly protonated form is stable and upshifts the cluster redox potential, enabling efficient water oxidation.
Keisuke Saito, Hiroshi Ishikita
wiley   +1 more source

The kinetic mechanism of bacterial ribosome recycling. [PDF]

, 2017
Bacterial ribosome recycling requires breakdown of the post-termination complex (PoTC), comprising a messenger RNA (mRNA) and an uncharged transfer RNA (tRNA) cognate to the terminal mRNA codon bound to the 70S ribosome.
Chen, Yuanwei, Cooperman, Barry S., Kaji, Akira, Kaji, Hideko   +3 more
core   +2 more sources

Damage‐limited resolution for X‐ray and electron microscopy of organic specimens

Acta Crystallographica Section D, Volume 82, Issue 5, Page 471-483, May 2026.
Analytical expressions for the damage‐limited resolution are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens. The findings will guide future microscopy and instrument design.Analytical expressions for the damage‐limited resolution (DLR) are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens ...
Ray F. Egerton, Colin Nave
wiley   +1 more source

Cryo-electron microscopy reveals human low density lipoprotein substructure.

Journal of Lipid Research, 1994
In the present study, we have examined the structure of human low density lipoprotein (LDL) using cryo-electron microscopy. Human LDL particles were analyzed in a vitrified frozen-hydrated condition, without chemical fixation or any form of staining ...
R Van Antwerpen, J C Gilkey
doaj   +1 more source