Results 71 to 80 of about 22,654 (207)

Integrative structural modelling and visualisation of a cellular organelle

QRB Discovery, 2022
Models of insulin secretory vesicles from pancreatic beta cells have been created using the cellPACK suite of tools to research, curate, construct and visualise the current state of knowledge. The model integrates experimental information from proteomics,
Ludovic Autin, Brett A. Barbaro, Andrew I. Jewett, Axel Ekman, Shruti Verma, Arthur J. Olson, David S. Goodsell   +6 more
doaj   +1 more source

Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture [PDF]

, 2018
Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the fine structural features of cellular compartments. In the present study, this approach has been applied in order to explore the ultrastructural morphology of ...
Bouchet-Marquis, C., Dubochet, J., Fakan, S.   +2 more
core  

The kinetic mechanism of bacterial ribosome recycling. [PDF]

, 2017
Bacterial ribosome recycling requires breakdown of the post-termination complex (PoTC), comprising a messenger RNA (mRNA) and an uncharged transfer RNA (tRNA) cognate to the terminal mRNA codon bound to the 70S ribosome.
Chen, Yuanwei, Cooperman, Barry S., Kaji, Akira, Kaji, Hideko   +3 more
core   +2 more sources

Bayesian perspective for orientation determination in cryo‐EM with application to structural heterogeneity analysis

Acta Crystallographica Section D, Volume 82, Issue 4, Page 274-294, April 2026.
A Bayesian perspective on orientation estimation in cryo‐EM is presented, with the minimum mean‐square error estimator outperforming standard cross‐correlation‐based approaches, particularly under challenging low signal‐to‐noise conditions. We demonstrate that improved orientation estimation has a decisive impact on 3D reconstruction quality and ...
Sheng Xu, Amnon Balanov, Amit Singer, Tamir Bendory   +3 more
wiley   +1 more source

Cryo-electron microscopy structure of a human PRMT5:MEP50 complex.

PLoS ONE, 2018
Protein arginine methyl transferase 5 (PRMT5) is a signaling protein and histone modifying enzyme that is important in many cellular processes, including regulation of eukaryotic gene transcription.
David E Timm, Valorie Bowman, Russell Madsen, Charles Rauch   +3 more
doaj   +1 more source

Fine Structure of Viral dsDNA Encapsidation [PDF]

, 2019
In vivo configurations of dsDNA of bacteriophage viruses in a capsid are known to form hexagonal chromonic liquid crystal phases. This article studies the liquid crystal ordering of viral dsDNA in an icosahedral capsid, combining the chromonic model with
Arsuaga, Javier, Calderer, M. Carme, Hiltner, Lindsey, Vazquez, Mariel, Walker, Shawn   +4 more
core   +3 more sources

Cryoelectron microscopy of mammalian pyruvate dehydrogenase complex.

Journal of Biological Chemistry, 1991
Cryoelectron microscopy has been performed on frozen-hydrated pyruvate dehydrogenase complexes from bovine heart and kidney and on various subcomplexes consisting of the dihydrolipoyl transacetylase-based (E2) core and substoichiometric levels of the other two major components, pyruvate dehydrogenase (E1) and dihydrolipoyl dehydrogenase (E3).
T, Wagenknecht, R, Grassucci, G A, Radke, T E, Roche   +3 more
openaire   +2 more sources

Trafficking of the human Na+/H+ antiporter NHA2 to the plasma membrane requires cornichon COPII cargo receptors

Protein Science, Volume 35, Issue 3, March 2026.
Abstract A key prerequisite of transporter proteins' function is their trafficking to the target cellular membranes where they fulfill distinct physiological roles. Cornichon proteins (CNIH/Erv14) represent a highly conserved family of coat protein complex II (COPII)‐coated vesicle cargo receptors that facilitate the exit of numerous transporters from ...
Karolína Kacovská, Klára Papoušková, Gal Masrati, Paul Rosas‐Santiago, Tereza Przeczková, Veronika Žárská, Nir Ben‐Tal, Olga Zimmermannová   +7 more
wiley   +1 more source

Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy [PDF]

Journal of Virology, 1994
Intracellular mature vaccinia virus, also called intracellular naked virus, and its core envelope have been observed in their native, unfixed, unstained, hydrated states by cryoelectron microscopy of vitrified samples. The virion appears as a smooth rounded rectangle of ca. 350 by 270 nm.
J, Dubochet, M, Adrian, K, Richter, J, Garces, R, Wittek   +4 more
openaire   +2 more sources

Breaking barriers: transitioning from X‐ray crystallography to cryo‐EM for structural studies

Acta Crystallographica Section D, Volume 82, Issue 3, Page 253-273, March 2026.
This article describes the transition of the Glass laboratory from X‐ray crystallography to single‐particle cryo‐electron microscopy (cryo‐EM) for structural studies of ATAD2B, a large AAA+ ATPase‐ and bromodomain‐containing protein involved in chromatin regulation.
Hassan Zafar, Kiera L. Malone, Ajit K. Singh, Michael A. Cianfrocco, Karen C. Glass   +4 more
wiley   +1 more source