Results 71 to 80 of about 17,043 (166)

Automated cryoelectron microscopy of “single particles” applied to the 26S proteasome [PDF]

FEBS Letters, 2007
The 26S proteasome is a large molecular machine with a central role in intracellular protein degradation in eukaryotes. The 2.5 MDa complex, which is built from two copies each of more than 30 different subunits, is labile and prone to dissociation into subcomplexes.
Nickell, S., Beck, F., Korinek, A., Mihalache, O., Baumeister, W., Plitzko, J.   +5 more
openaire   +3 more sources

Histidine Protonation and the Oxidizing Power of the Oxygen‐Evolving Complex

ChemPhotoChem, Volume 10, Issue 5, May 2026.
Protonation of D1‐His337 governs the oxidizing power of the Mn4CaO5 cluster in photosystem II. Its singly protonated form is unstable, resulting in histidine oxidation and disruption of Mn oxidation, whereas the doubly protonated form is stable and upshifts the cluster redox potential, enabling efficient water oxidation.
Keisuke Saito, Hiroshi Ishikita
wiley   +1 more source

Damage‐limited resolution for X‐ray and electron microscopy of organic specimens

Acta Crystallographica Section D, Volume 82, Issue 5, Page 471-483, May 2026.
Analytical expressions for the damage‐limited resolution are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens. The findings will guide future microscopy and instrument design.Analytical expressions for the damage‐limited resolution (DLR) are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens ...
Ray F. Egerton, Colin Nave
wiley   +1 more source

Steroid Recognition in Adhesion GPCRs: A Structural and Pharmacological Perspective

Pharmacology Research &Perspectives, Volume 14, Issue 2, April 2026.
ABSTRACT Emerging evidence suggests steroids may act as generalizable ligands for adhesion GPCRs, a receptor class with considerable pharmacological potential. While most studies have focused on the ADGR‘G' subfamily, recent cryo‐EM structures of the androgens 5α‐dihydrotestosterone (5α‐DHT) and methenolone bound to ADGRD1 revealed a putative steroid ...
Bethany Fleming, Abdul‐Akim Guseinov, Irina G. Tikhonova, Graeme Milligan, Nicole A. Perry‐Hauser   +4 more
wiley   +1 more source

Precise Capture of Membrane Proteins Using DNA‐Origami‐Constrained Nanodiscs

Small Structures, Volume 7, Issue 4, April 2026.
A designed DNA origami structure ensures manipulates and inserts an individual membrane protein in a single, captured nanodisc. Membrane proteins play a key role as cellular gatekeepers, regulating the flow of information and material across lipid membranes.
Piotr Stepien, Gerrit Wilkens, Sylwia Swiatek, Manuel Yusef Robles, Anna Swietlikowska, Sarah Hutchings, Dmitry Ghilarov, Jonathan G. Heddle   +7 more
wiley   +1 more source

Structures of pentatricopeptide repeat proteins

Acta Crystallographica Section F, Volume 82, Issue 4, Page 101-113, April 2026.
A number of crystal, cryo‐EM and solution structures of pentatricopeptide repeat proteins have been solved, revealing details of how they interact with single‐stranded RNA and other proteins, as well as their conformational repertoire.Pentatricopeptide repeat (PPR) proteins constitute the largest family of RNA‐binding proteins in land plants, playing ...
Anuradha Pullakhandam, Crystal Cooper, Ian D. Small, Charles S. Bond   +3 more
wiley   +1 more source

Bayesian perspective for orientation determination in cryo‐EM with application to structural heterogeneity analysis

Acta Crystallographica Section D, Volume 82, Issue 4, Page 274-294, April 2026.
A Bayesian perspective on orientation estimation in cryo‐EM is presented, with the minimum mean‐square error estimator outperforming standard cross‐correlation‐based approaches, particularly under challenging low signal‐to‐noise conditions. We demonstrate that improved orientation estimation has a decisive impact on 3D reconstruction quality and ...
Sheng Xu, Amnon Balanov, Amit Singer, Tamir Bendory   +3 more
wiley   +1 more source

Trafficking of the human Na+/H+ antiporter NHA2 to the plasma membrane requires cornichon COPII cargo receptors

Protein Science, Volume 35, Issue 3, March 2026.
Abstract A key prerequisite of transporter proteins' function is their trafficking to the target cellular membranes where they fulfill distinct physiological roles. Cornichon proteins (CNIH/Erv14) represent a highly conserved family of coat protein complex II (COPII)‐coated vesicle cargo receptors that facilitate the exit of numerous transporters from ...
Karolína Kacovská, Klára Papoušková, Gal Masrati, Paul Rosas‐Santiago, Tereza Przeczková, Veronika Žárská, Nir Ben‐Tal, Olga Zimmermannová   +7 more
wiley   +1 more source

Cryoelectron microscopy pioneers win chemistry Nobel [PDF]

Physics Today, 2017
Proteins frozen in vitreous ice are now being imaged with atomic resolution.
openaire   +1 more source

Structural heterogeneity of apoB-containing serum lipoproteins visualized using cryo-electron microscopy

Journal of Lipid Research, 1999
Cryo-electron microscopy was used to analyze the structure of lipoprotein particles in density gradient subfractions of human very low density lipoprotein (VLDL), intermediate density lipoprotein (IDL), and low density lipoprotein (LDL).
Rik van Antwerpen, Michael La Belle, Edita Navratilova, Ronald M. Krauss   +3 more
doaj   +1 more source