Results 91 to 100 of about 22,940 (199)

Tropomyosin Positions in Regulated Thin Filaments Revealed by Cryoelectron Microscopy [PDF]

Biophysical Journal, 1999
Past attempts to detect tropomyosin in electron micrograph images of frozen-hydrated troponin-regulated thin filaments under relaxing conditions have not been successful. This raised the possibility that tropomyosin may be disordered on filaments in the off-state, a possibility at odds with the steric blocking model of muscle regulation.
Xu, C., Craig, R., Tobacman, L., Horowitz, R., Lehman, W.   +4 more
openaire   +2 more sources

Bayesian perspective for orientation determination in cryo‐EM with application to structural heterogeneity analysis

Acta Crystallographica Section D, Volume 82, Issue 4, Page 274-294, April 2026.
A Bayesian perspective on orientation estimation in cryo‐EM is presented, with the minimum mean‐square error estimator outperforming standard cross‐correlation‐based approaches, particularly under challenging low signal‐to‐noise conditions. We demonstrate that improved orientation estimation has a decisive impact on 3D reconstruction quality and ...
Sheng Xu, Amnon Balanov, Amit Singer, Tamir Bendory   +3 more
wiley   +1 more source

Protocol for the purification of replisomes from the Xenopus laevis egg extract system for single-particle cryo-EM analysis

STAR Protocols
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract.
Paolo Passaretti, Milos A. Cvetkovic, Alessandro Costa, Agnieszka Gambus   +3 more
doaj   +1 more source

Advances in Structure Modeling Methods for Cryo-Electron Microscopy Maps

Molecules, 2019
Cryo-electron microscopy (cryo-EM) has now become a widely used technique for structure determination of macromolecular complexes. For modeling molecular structures from density maps of different resolutions, many algorithms have been developed.
Eman Alnabati, Daisuke Kihara
doaj   +1 more source

Howard Hughes Medical Institute 2013 Year in Review [PDF]

, 2014
The Howard Hughes Medical Institute is the nation's largest private supporter of academic biomedical research. As a medical research organization, the Institute spends at least 3.5 percent of its endowment each year on research activities and related ...

core  

Cryoelectron microscopy of low density lipoprotein in vitreous ice

Biophysical Journal, 1995
In this report, images of low density lipoprotein (LDL) in vitreous ice at approximately 30 A resolution are presented. These images show that LDL is a quasi-spherical particle, approximately 220-240 A in diameter, with a region of low density (lipid) surrounded by a ring (in projection) of high density believed to represent apolipoprotein B-100.
Spin, J.M., Atkinson, D.
openaire   +2 more sources

Trafficking of the human Na+/H+ antiporter NHA2 to the plasma membrane requires cornichon COPII cargo receptors

Protein Science, Volume 35, Issue 3, March 2026.
Abstract A key prerequisite of transporter proteins' function is their trafficking to the target cellular membranes where they fulfill distinct physiological roles. Cornichon proteins (CNIH/Erv14) represent a highly conserved family of coat protein complex II (COPII)‐coated vesicle cargo receptors that facilitate the exit of numerous transporters from ...
Karolína Kacovská, Klára Papoušková, Gal Masrati, Paul Rosas‐Santiago, Tereza Przeczková, Veronika Žárská, Nir Ben‐Tal, Olga Zimmermannová   +7 more
wiley   +1 more source

Ordering in dense fiber bundles, the phyllotactic solution and its application to collagen fibrils

, 2013
The shape of the cross section of a dense fiber bundle is related to the symmetry of its molecular packing. However, this statement might be belied by type I collagen fibrils which have a rounded section of high symmetry while structural studies suggest ...
Charvolin, Jean, Sadoc, Jean-François
core   +1 more source

Breaking barriers: transitioning from X‐ray crystallography to cryo‐EM for structural studies

Acta Crystallographica Section D, Volume 82, Issue 3, Page 253-273, March 2026.
This article describes the transition of the Glass laboratory from X‐ray crystallography to single‐particle cryo‐electron microscopy (cryo‐EM) for structural studies of ATAD2B, a large AAA+ ATPase‐ and bromodomain‐containing protein involved in chromatin regulation.
Hassan Zafar, Kiera L. Malone, Ajit K. Singh, Michael A. Cianfrocco, Karen C. Glass   +4 more
wiley   +1 more source

An assessment of the resolution limitation due to radiation-damage in x-ray diffraction microscopy

, 2005
X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities.
Barty, Baruchel, Beetz, Burmeister, C. Cui, C.J. Jacobsen, Chapman, Chapman, D. Sayre, D. Starodub, D.A. Shapiro, E. Lima, Elser, Fienup, Franck, Garman, Garman, Gerchberg, Glaeser, Glaeser, Glaeser, Goodman, H. Miao, H.N. Chapman, Hayward, Hegerl, Henderson, Henderson, Henke, J. Kirz, J.C.H. Spence, J.M. Holton, Jacobsen, Kirz, Kmetko, London, M.R. Howells, Marchesini, Marchesini, Maser, McEwen, Medalia, Miao, Miao, Miao, Miao, Mueller, Nave, Neutze, Owen, Plitzko, Ravelli, Reimer, Robinson, Rodenburg, Rudolph, S. Marchesini, Sayre, Sayre, Sayre, Schlotter, Schneider, Shapiro, Shen, Sliz, Spence, Spence, Starodub, T. Beetz, Williams, Zhang   +70 more
core   +1 more source