Results 81 to 90 of about 22,654 (207)

Cryo-electron microscopy reveals human low density lipoprotein substructure.

Journal of Lipid Research, 1994
In the present study, we have examined the structure of human low density lipoprotein (LDL) using cryo-electron microscopy. Human LDL particles were analyzed in a vitrified frozen-hydrated condition, without chemical fixation or any form of staining ...
R Van Antwerpen, J C Gilkey
doaj   +1 more source

Surface topography of microtubule walls decorated with monomeric and dimeric kinesin constructs [PDF]

, 2000
The surface topography of opened-up microtubule walls (sheets) decorated with monomeric and dimeric kinesin motor domains was investigated by freeze-drying and unidirectional metal shadowing.
Doerhoefer, Monika, Gross, Heinz, Hoenger, Andreas, Mandelkow, Eckhard, Song, Young Hwa, Tittmann, Peter, Woehlke, Guenther   +6 more
core   +2 more sources

Radiation‐dose effects in correlative X‐ray/cryo‐electron microscopy of frozen‐hydrated biological samples

Acta Crystallographica Section D, Volume 82, Issue 3, Page 207-215, March 2026.
Cryo‐EM analysis of apoferritin exposed to an X‐ray dose of 100 MGy confirms the potential for high‐resolution correlative imaging workflows combining X‐ray tomography and cryo‐EM.In cryo‐electron microscopy (cryo‐EM), imaging of biological specimens is restricted by the limited field of view and by sample thickness.
Thorsten B. Blum, Vincent Olieric, Ana Diaz, Takashi Ishikawa, Volodymyr M. Korkhov   +4 more
wiley   +1 more source

Cryoelectron Microscopy and Image Analysis of the Cardiac Ryanodine Receptor [PDF]

Journal of Biological Chemistry, 1998
The three-dimensional structure of the cardiac muscle ryanodine receptor (RyR2) is described and compared with its skeletal muscle isoform (RyR1). Previously, structural studies of RyR2 have not been as informative as those for RyR1 because optimal conditions for electron microscopy, which require low levels of phospholipid, are destabilizing for RyR2.
M R, Sharma, P, Penczek, R, Grassucci, H B, Xin, S, Fleischer, T, Wagenknecht   +5 more
openaire   +2 more sources

Associating protein residues in the literature with structural data

Acta Crystallographica Section D, Volume 82, Issue 2, Page 113-125, February 2026.
A software tool is introduced for associating text mentions of protein residues with their respective residues in a protein structure, with display in a molecular viewer.Protein structures are crucial in understanding the function, mechanism and disease‐causing variants of proteins within any living cell.
Melanie Vollmar, Simon Westrip, Sreenath Nair, Balakumaran Balasubramaniyan, Sameer Velankar, Louise Jones, Peter Strickland   +6 more
wiley   +1 more source

A heterologous expression system for bovine lens transmembrane main intrinsic protein (MIP) in Nicotiana tabacum plants [PDF]

, 1999
We have developed a heterologous expression system for transmembrane lens main intrinsic protein (MIP) in Nicotiana tabacum plant tissue. A native bovine MIP26 amplicon was subcloned into an expression cassette under the control of a constitutive ...
Crabbe, M. J. C., de Peyer, O. S., Dunwell, Jim M., Wetten, Andrew C.   +3 more
core  

Radial distribution of RNA genome packaged inside spherical viruses

, 2008
The problem of RNA genomes packaged inside spherical viruses is studied. The viral capsid is modeled as a hollowed sphere. The attraction between RNA molecules and the inner viral capsid is assumed to be non-specific and occurs at the inner capsid ...
J. Johnson, P.-G. de Gennes, Se Il Lee, Toan T. Nguyen   +3 more
core   +1 more source

Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy

Journal of Microscopy, Volume 301, Issue 2, Page 168-186, February 2026.
Abstract Malaria is one of the deadliest infectious diseases in the world, annually responsible for over 400,000 deaths. It is caused by parasites of the genus Plasmodium, which undergo remarkable structural changes during their development within different cells across various hosts.
Rachel Rachid, Camila Wendt, Wanderley de Souza, Kildare Miranda   +3 more
wiley   +1 more source

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex

Cell Reports, 2019
Summary: LSD1 and LSD2 are homologous histone demethylases with opposite biological outcomes related to chromatin silencing and transcription elongation, respectively.
Chiara Marabelli, Biagina Marrocco, Simona Pilotto, Sagar Chittori, Sarah Picaud, Sara Marchese, Giuseppe Ciossani, Federico Forneris, Panagis Filippakopoulos, Guy Schoehn, Daniela Rhodes, Sriram Subramaniam, Andrea Mattevi   +12 more
doaj   +1 more source

Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

Nature Communications, 2020
For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information.
Steven Z. Chou, Thomas D. Pollard
doaj   +1 more source