Results 81 to 90 of about 1,110,843 (197)

Protocol for the purification of replisomes from the Xenopus laevis egg extract system for single-particle cryo-EM analysis

STAR Protocols
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract.
Paolo Passaretti, Milos A. Cvetkovic, Alessandro Costa, Agnieszka Gambus   +3 more
doaj   +1 more source

Advances in Structure Modeling Methods for Cryo-Electron Microscopy Maps

Molecules, 2019
Cryo-electron microscopy (cryo-EM) has now become a widely used technique for structure determination of macromolecular complexes. For modeling molecular structures from density maps of different resolutions, many algorithms have been developed.
Eman Alnabati, Daisuke Kihara
doaj   +1 more source

Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

Nature Communications, 2020
For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information.
Steven Z. Chou, Thomas D. Pollard
doaj   +1 more source

Cryo‐EM structure of ALC1 in an open conformation bound to a PARylated nucleosome

Acta Crystallographica Section D, Volume 82, Issue 6, Page 683-699, June 2026.
Reanalysis of a publicly available cryo‐EM dataset identifies a new conformation of the oncogenic chromatin remodeler ALC1/CHD1L bound to a PARylated nucleosome. This new structure reveals the position of the macro domain in the complex, and may help to understand the large conformational change leading to the activation of ALC1.Nucleosomes are the ...
Hannah R. Bridges, Luka Bacic, Sebastian Deindl, Guillaume Gaullier   +3 more
wiley   +1 more source

The evolving role of structural biology in pharma: integration of X‐ray crystallography, cryo‐electron microscopy and beyond

Acta Crystallographica Section D, Volume 82, Issue 6, Page 587-602, June 2026.
This review describes how an integrated structural biology platform at Merck Sharp & Dohme LLC, Rahway, New Jersey, USA, combining X‐ray crystallography, cryo‐EM, MicroED and cryo‐ET, enables seamless three‐dimensional insights from molecular to cellular length scales.
Jill E. Chrencik, Hua-Poo Su, Yacob Gomez Llorente, Rachel L. Palte, Daniel J. Klein, Robert P. Hayes, Sadie P. Antine, Guilermo A. Asmar-Rovira, Nicole Bertoletti, Noel J. Byrne, Brittany L. Carroll, Ciro D. Cordeiro, Kaylynn Curfman, James Dutko, Michael J. Eddins, Thierry Fischmann, Barani Govindarajan, Corey W. Hecksel, Thu Ho, Kaspar Hollenstein, Mee Ra Hong, Alan Hruza, Andrii Ishchenko, Maru Jaime-Garza, Matt J. Jaremko, Hayley L. Knox, James Kostas, Harini Krishnamurthy, Ioannis Manolaridis, Cameron L. Noland, Andrea T. Partridge, Sangita B. Patel, Paul Reichert, John C. Reid, Catarina Felisberto-Rodrigues, Chitra A. Shintre, Jennifer M. Shipman, Elakkiya Tamilselvan, Morgan E. Walker, Susanne N. Walker, Renjing Wang, Katharine M. Wright, Tien-Jui Yen, Byung-Kuk Yoo, Sandra B. Gabelli   +44 more
wiley   +1 more source

Cryoelectron microscopy of low density lipoprotein in vitreous ice

Biophysical Journal, 1995
In this report, images of low density lipoprotein (LDL) in vitreous ice at approximately 30 A resolution are presented. These images show that LDL is a quasi-spherical particle, approximately 220-240 A in diameter, with a region of low density (lipid) surrounded by a ring (in projection) of high density believed to represent apolipoprotein B-100.
Spin, J.M., Atkinson, D.
openaire   +2 more sources

Use of confocal and multiphoton microscopy for the evaluation of micro-optical components and emitters [PDF]

, 2004
We report on the application of confocal and multiphoton microscopic techniques for the evaluation of the latest generation of micro optical components.
Choi, H.W., McConnell, G., McConnell, G, E. Gu, Gu, E., C. Griffin, G. McConnell, M.D. Dawson, Dawson, M.D., Griffin, C., Dawson, MD, Girkin, JM, H.W. Choi, Griffin, C, Girkin, J.M., Gu, E, J.M. Girkin, Choi, HW   +17 more
core   +1 more source

Dynamic LHCI antenna and inter‐photosystem spillover enable flexible light harvesting in Euglena gracilis

The Plant Journal, Volume 126, Issue 5, June 2026.
Significance Statement Euglena gracilis is an early diverging photosynthetic organism with a unique mosaic photosynthetic apparatus. Biochemical and spectroscopic analysis reveals a kinetically slower photosystem I sharing excitation energy with photosystem II in the absence of strict photosystem segregation.
Parveen Akhtar, László Kovács, Ildikó Domonkos, Ádám Pap, Aladár Pettkó‐Szandtner, Soujanya Kuntam, Petar H. Lambrev   +6 more
wiley   +1 more source

Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike [PDF]

, 2013
The genus <i>Orthobunyavirus</i> within the family <i>Bunyaviridae</i> constitutes an expanding group of emerging viruses, which threaten human and animal health.
McLees, A., Huiskonen, JT, Yeromonahos, C, Christelle Yeromonahos (413081), Juha T. Huiskonen (191734), David Bitto, David Bitto (413079), Bowden, Thomas A, Elliott, RM, Elliott, Richard M., Richard M. Elliott (252934), Elliott, Richard, Bowden Thomas A., McLees, A, Yeromonahos, Christelle, Huiskonen, Juha T., Angela McLees (413080), Elliott, Richard M, Christelle Yeromonahos, Pierson, Ted, Huiskonen, J.T., Elliott, R.M., Huiskonen, Juha, Richard M Elliott, McLees, Angela, Bowden, T.A., Bitto, David, Bitto David, Bitto, D, Huiskonen, Juha T, Elliott Richard M., Bowden, Thomas A., Thomas A. Bowden (413078), McLees Angela, Bitto, D., Bowden, Thomas, Huiskonen Juha T., Angela McLees, Bowden, TA, Yeromonahos, C., Thomas A Bowden, Yeromonahos Christelle, Juha T Huiskonen   +42 more
core   +1 more source

Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge

Cell Reports
Summary: Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering ...
Jitendra Maharana, Chun-Hsiung Wang, Li-An Tsai, Yi-Ting Liao, Cheng-Han Yang, Melvin C. Shen, Lourriel S. Macale, Thang Ngoc Tran, Joemark Narsico, Ronelito J. Perez, Sunil Kumar Tewary, Jian-Li Wu, Hong-You Lin, Shu-Wei Chang, Aaron Franklin, Patrick J. Moynihan, Deborah Jacobs-Sera, Krista G. Freeman, Graham F. Hatfull, Todd L. Lowary, Meng-Chiao Ho   +20 more
doaj   +1 more source