Results 21 to 30 of about 2,310,044 (293)

COVID-19 serological survey using micro blood sampling

Scientific Reports, 2021
During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of ...
Melissa M. Matthews, Tae Gyun Kim, Satoshi Shibata, Noriko Shibata, Christian Butcher, Jaekyung Hyun, Keon Young Kim, Theodore Robb, Siang Sheng Jheng, Masashi Narita, Tomoari Mori, Mary Collins, Matthias Wolf   +12 more
doaj   +1 more source

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source

Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy

Cell Discovery, 2022
Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical ...
Aojie Wang, Feng Zhou, Congcong Liu, Dongsheng Gao, Ruxi Qi, Yiheng Yin, Sheng Liu, Yuanzhu Gao, Lutang Fu, Yinhe Xia, Yawei Xu, Chuanqing Wang, Zheng Liu   +12 more
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High-throughput high-resolution cryo-electron crystallography [PDF]

, 2015
High-resolution structures of membrane and soluble proteins can be obtained by cryo-electron crystallography, given highly-ordered cryo-preparations of perfectly flat 2D crystals are available.
Scherer, Sebastian
core   +1 more source

`Cryo-EM': electron cryomicroscopy, cryo electron microscopy or something else?

IUCrJ, 2023
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Richard Henderson, Samar Hasnain
doaj   +1 more source

CRYO-ELECTRON MICROSCOPY OF SPLICEOSOMAL COMPONENTS

Annual Review of Biophysics and Biomolecular Structure, 2006
Splicing is an essential step of gene expression in which introns are removed from pre-mRNA to generate mature mRNA that can be translated by the ribosome. This reaction is catalyzed by a large and dynamic macromolecular RNP complex called the spliceosome. The spliceosome is formed by the stepwise integration of five snRNPs composed of U1, U2, U4, U5,
Stark, Holger, Lührmann, Reinhard
openaire   +4 more sources

Adapting cryogenic correlative light and electron microscopy (cryo-CLEM) for food oxidation studies [PDF]

, 2023
Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox ...
Johannes, Hohlbein, Suyeon, Yang, John, van Duynhoven, Heiner, Friedrich, Rick, Joosten, Machi, Takeuchi   +5 more
core   +1 more source

Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
doaj   +1 more source

The cryo-electron microscopy structure of huntingtin [PDF]

Nature, 2018
Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development and is involved in diverse cellular activities such as vesicular transport, endocytosis, autophagy and the regulation of transcription. Although an integrative understanding of the biological functions of HTT is lacking, the large number of identified HTT ...
Guo, Q., Huang, B., Cheng, J., Seefelder, M., Engler, T., Pfeifer, G., Oeckl, P., Otto, M., Moser, F., Maurer, M., Pautsch, A., Baumeister, W., Fernandez-Busnadiego, R., Kochanek, S.   +13 more
openaire   +4 more sources

Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators

Nature Communications, 2022
The Kv1.3 potassium channel is expressed abundantly on activated T cells and mediates the cellular immune responses. Here, the authors report structures of the Kv1.3 potassium channel with and without immunoglobulin modulators, shedding light on the ...
Purushotham Selvakumar, Ana I. Fernández-Mariño, Nandish Khanra, Changhao He, Alice J. Paquette, Bing Wang, Ruiqi Huang, Vaughn V. Smider, William J. Rice, Kenton J. Swartz, Joel R. Meyerson   +10 more
doaj   +1 more source