Results 21 to 30 of about 2,439,094 (286)
Assembly mechanism of the pleomorphic immature poxvirus scaffold
Nature Communications, 2022 Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun +3 more
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Nature Communications, 2023 The portal-scaffold complex is believed to nucleate the assembly of herpesvirus procapsids. During capsid maturation, two events occur: scaffold expulsion and DNA incorporation. The portal-scaffold interaction and the conformational changes that occur to
Zhihai Li +5 more
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Cohomology of Cryo-Electron Microscopy [PDF]
SIAM Journal on Applied Algebra and Geometry, 2017 The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this article, we show that the basic premise of cryo-EM --- patching together 2-dimensional projections to reconstruct a 3-dimensional object --- is naturally one of Cech cohomology with SO(2)-
Ke Ye, Lek-Heng Lim
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Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G
Nature Communications, 2023 Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV’s counteraction mechanism to evade antiviral innate immune response.
Takahide Kouno +6 more
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COVID-19 serological survey using micro blood sampling
Scientific Reports, 2021 During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of ...
Melissa M. Matthews +12 more
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Temporal dynamics of charge buildup in cryo-electron microscopy
Journal of Structural Biology: X, 2023 It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber +4 more
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Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy
Cell Discovery, 2022 Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical ...
Aojie Wang +12 more
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Structural basis for genome packaging, retention, and ejection in human cytomegalovirus
Nature Communications, 2021 Human cytomegalovirus (HCMV) is the prototypical member of the β-herpesvirinae subfamily and the leading viral cause of congenital infections that can lead to birth defects and it can also cause life-threatening disease in immunocompromised individuals ...
Zhihai Li +3 more
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Cryo-electron microscopy of viruses [PDF]
Nature, 1984 Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional
Adrian, Marc +3 more
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Nature Communications, 2023 The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral
Rafael Ayala +9 more
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