Results 51 to 60 of about 2,310,044 (293)

The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes

Nature Communications, 2016
Continuous cycles of nucleo-cytoplasmic transport require disassembly of transport receptor-Ran-GTP complexes in the cytoplasm. Here the authors elucidate the specific function of the RanBP2 complex in the disassembly process.
Tobias Ritterhoff, Hrishikesh Das, Götz Hofhaus, Rasmus R. Schröder, Annette Flotho, Frauke Melchior   +5 more
doaj   +1 more source

Mechanisms and therapeutic opportunities of the ribotoxic stress response in cancer

Molecular Oncology, EarlyView.
Cancer cells' high translational demand creates opportunities to therapeutically target ribosome function. Ribosome stalling and collisions activate ZAKα and the ribotoxic stress response (RSR), which can trigger rapid, p53‐independent apoptosis in cancer.
Anastassiya Kim, Maryam Aslam, Elouise Torruella, Alphons Sony, Asif Rahman, Simira Smith, Columba de la Parra, Moira Sauane   +7 more
wiley   +1 more source

Thermoresponsive Hydrogels with Improved Actuation Function by Interconnected Microchannels

Advanced Intelligent Systems, 2022
Stimuli−responsive hydrogels are important in soft actuators research, as they change volume in response to environmental factors. Thermoresponsive hydrogels, such as poly(N‐isopropylacrylamide) (pNIPAM), typically have slow response rates and exert ...
Tobias Spratte, Christine Arndt, Irene Wacker, Margarethe Hauck, Rainer Adelung, Rasmus R. Schröder, Fabian Schütt, Christine Selhuber-Unkel   +7 more
doaj   +1 more source

Cryo‐electron microscopy of vitrified chromosomes in situ. [PDF]

The EMBO Journal, 1986
Chromosomes of metaphase-arrested Chinese hamster ovary (CHO) and HeLa cells were examined in situ, unfixed and unstained, by cryo-electron microscopy. In hydrated, vitrified cryo-sections, chromosomes exhibit a characteristic homogeneous, grainy texture, which, on optical diffraction, gives rise to a broad reflection corresponding to 11 nm.
McDowall, A.W., Smith, J.M., Dubochet, J.   +2 more
openaire   +5 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. [PDF]

, 2015
Despite the great progress recently made in resolving their structures, investigation of the structural biology of membrane proteins still presents major challenges.
Shaun Rawson, Mitchell, JK, Postis, Vincent; id_orcid, Vincent Postis, Tim R Dafforn, Postis, Vincent, Muench, Stephen P., Baldwin, SA, Rawson, Shaun, Baldwin, Stephen A., Postis, V, Rawson, S, Stephen A Baldwin, Lee, SC, Rosemary A Parslow, Mitchell, Jennifer K., Dafforn, TR, Lee, Sarah C., Parslow, Rosemary A., Jennifer K Mitchell, Sarah C Lee, Stephen P Muench, Muench, SP, Dafforn, Tim R., Parslow, RA   +24 more
core   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Engineered protein subunit COVID19 vaccine is as immunogenic as nanoparticles in mouse and hamster models

Scientific Reports
Initial studies on the immunogenicity of SARS-CoV-2 (CoV-2) S glycoprotein (“spike”) as a protein subunit vaccine suggested sub-optimal efficacy in mammals.
Melissa M. Matthews, Tae Gyun Kim, Keon Young Kim, Vladimir Meshcheryakov, Higor Alves Iha, Miho Tamai, Daiki Sasaki, Paola Laurino, Saacnicteh Toledo-Patiño, Mary Collins, Tzung-Yang Hsieh, Satoshi Shibata, Noriko Shibata, Fumiko Obata, Jun Fujii, Toshihiro Ito, Hiroshi Ito, Hiroki Ishikawa, Matthias Wolf   +18 more
doaj   +1 more source

Optimizing weighting functions for cryo-electron microscopy

Biophysics Reports, 2021
The frequency-dependent signal to noise ratio of cryo-electron microscopy data varies dramatically with the frequency and with the type of the data. During different steps of data processing, data with distinct SNR are used for calculations. Thus, specific weighting function based on the particular SNR should be designed to optimize the corresponding ...
Cheng, Jing, Zhang, Xinzheng
openaire   +2 more sources

Combined approaches to flexible fitting and assessment in virus capsids undergoing conformational change [PDF]

, 2013
Fitting of atomic components into electron cryo-microscopy (cryoEM) density maps is routinely used to understand the structure and function of macromolecular machines.
Shakeel, S., Topf, Maya, Sarah Jane Butcher, Pandurangan, A.P., Shabih Shakeel, Arun Prasad Pandurangan, Pandurangan, Arun Prasad, Butcher, S., Shakeel, Shabih, Butcher, Sarah Jane, Maya Topf   +10 more
core   +1 more source