Results 71 to 80 of about 133,701 (285)

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Potential therapeutic targeting of BKCa channels in glioblastoma treatment

Molecular Oncology, EarlyView.
This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak, Karolina Pytlak, Sandra Jaworowska, Bogusz Kulawiak, Piotr Bednarczyk   +4 more
wiley   +1 more source

Engineered protein subunit COVID19 vaccine is as immunogenic as nanoparticles in mouse and hamster models

Scientific Reports
Initial studies on the immunogenicity of SARS-CoV-2 (CoV-2) S glycoprotein (“spike”) as a protein subunit vaccine suggested sub-optimal efficacy in mammals.
Melissa M. Matthews, Tae Gyun Kim, Keon Young Kim, Vladimir Meshcheryakov, Higor Alves Iha, Miho Tamai, Daiki Sasaki, Paola Laurino, Saacnicteh Toledo-Patiño, Mary Collins, Tzung-Yang Hsieh, Satoshi Shibata, Noriko Shibata, Fumiko Obata, Jun Fujii, Toshihiro Ito, Hiroshi Ito, Hiroki Ishikawa, Matthias Wolf   +18 more
doaj   +1 more source

Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton [PDF]

, 2011
Microtubules play crucial roles in cytokinesis, transport, and motility, and are therefore superb targets for anti-cancer drugs. All tubulins evolved from a common ancestor they share with the distantly related bacterial cell division protein FtsZ, but ...
A Briegel, A Marchler-Bauer, A. J Koster, A. J Martin-Galiano, Alasdair W. McDowall, B Miroux, C Jenkins, C Suloway, C. A Sontag, C. A Sontag, C. H Aylett, C. V Iancu, D Bermudes, D Nicastro, D Schlieper, D. N Mastronarde, D. N Mastronarde, D. P Devos, E Nogales, E Nogales, E Nogales, E Nogales, E. F Pettersen, E. G Reynaud, E. I Tocheva, F Amat, Giulio Petroni, Grant J. Jensen, H Erickson, H Sui, H. P Erickson, J Cope, J Lowe, J. B Heymann, J. D Thompson, J. D Wisotzkey, J. O McInerney, J. R McIntosh, K. C Lee, K. S Makarova, K. T Tokuyasu, K. T Tokuyasu, L Gan, L Ni, L. A Amos, L. A Amos, Linda Amos, M Pilhofer, M Pilhofer, M Pilhofer, M Takeda, M Wagner, M. S Ladinsky, M. S Ladinsky, Mark S. Ladinsky, Martin Pilhofer, P Forterre, P. J Keeling, R. I Koning, S Vaughan, S. K Dutcher, S. Q Zheng, T Muller, T. K Attwood, W Ludwig, W. F Tivol, Z Li   +66 more
core   +5 more sources

Cryo-electron Microscopy of Adeno-associated Virus

Chemical Reviews, 2022
Adeno-associated virus (AAV) has a single-stranded DNA genome encapsidated in a small icosahedrally symmetric protein shell with 60 subunits. AAV is the leading delivery vector in emerging gene therapy treatments for inherited disorders, so its structure and molecular interactions with human hosts are of intense interest.
Scott M. Stagg, Craig Yoshioka, Omar Davulcu, Michael S. Chapman   +3 more
openaire   +2 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Optimizing weighting functions for cryo-electron microscopy

Biophysics Reports, 2021
The frequency-dependent signal to noise ratio of cryo-electron microscopy data varies dramatically with the frequency and with the type of the data. During different steps of data processing, data with distinct SNR are used for calculations. Thus, specific weighting function based on the particular SNR should be designed to optimize the corresponding ...
Cheng, Jing, Zhang, Xinzheng
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy

Nature Communications, 2020
Microtubule (MT) organization is regulated by many microtubule-associated proteins (MAPs) that contain intrinsically disordered regions. Here authors produce [13C, 15N] labeled, functional microtubules from human cells for solid-state NMR which allows ...
Yanzhang Luo, ShengQi Xiang, Peter Jan Hooikaas, Laura van Bezouwen, A. S. Jijumon, Carsten Janke, Friedrich Förster, Anna Akhmanova, Marc Baldus   +8 more
doaj   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source