Results 61 to 70 of about 2,439,094 (286)

Orthogonal matrix retrieval in cryo-electron microscopy [PDF]

2015 IEEE 12th International Symposium on Biomedical Imaging (ISBI), 2015
In single particle reconstruction (SPR) from cryo-electron microscopy (cryo-EM), the 3D structure of a molecule needs to be determined from its 2D projection images taken at unknown viewing directions. Zvi Kam showed already in 1980 that the autocorrelation function of the 3D molecule over the rotation group SO(3) can be estimated from 2D projection ...
Tejal Bhamre, Teng Zhang 0002, Amit Singer   +2 more
openaire   +5 more sources

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Cryo-EM structural determination of PfFNT.

, 2021
(A) A representative cryo-EM micrograph of PfFNT. (B) A representative 2D classification average. (C) Flowchart for EM data processing of PfFNT datasets. Details can be found in the Methods. cryo-EM, cryogenic-electron microscopy; EM, electron microscopy;
Yanxia Zhou (1447465), Jialu Li (506281), Huaichuan Duan (6458477), Nan Wang (21935), Chen Wang (88408), Dong Deng (8202849), Qingjie Xiao (9226140), Li Guo (171454), Weidan Yuan (10711212), Xin Jiang (6348), Zhi-jun Jia (11413904), Jianping Hu (287208), Chuangye Yan (554009), Xi Peng (406437), Angqi Zhu (8681169), Jia Geng (1708321), Hanwen Xu (6332030), Fei Liu (21082)   +17 more
core   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes

Nature Communications, 2016
Continuous cycles of nucleo-cytoplasmic transport require disassembly of transport receptor-Ran-GTP complexes in the cytoplasm. Here the authors elucidate the specific function of the RanBP2 complex in the disassembly process.
Tobias Ritterhoff, Hrishikesh Das, Götz Hofhaus, Rasmus R. Schröder, Annette Flotho, Frauke Melchior   +5 more
doaj   +1 more source

Thermoresponsive Hydrogels with Improved Actuation Function by Interconnected Microchannels

Advanced Intelligent Systems, 2022
Stimuli−responsive hydrogels are important in soft actuators research, as they change volume in response to environmental factors. Thermoresponsive hydrogels, such as poly(N‐isopropylacrylamide) (pNIPAM), typically have slow response rates and exert ...
Tobias Spratte, Christine Arndt, Irene Wacker, Margarethe Hauck, Rainer Adelung, Rasmus R. Schröder, Fabian Schütt, Christine Selhuber-Unkel   +7 more
doaj   +1 more source

Cryo‐electron microscopy of vitrified chromosomes in situ. [PDF]

The EMBO Journal, 1986
Chromosomes of metaphase-arrested Chinese hamster ovary (CHO) and HeLa cells were examined in situ, unfixed and unstained, by cryo-electron microscopy. In hydrated, vitrified cryo-sections, chromosomes exhibit a characteristic homogeneous, grainy texture, which, on optical diffraction, gives rise to a broad reflection corresponding to 11 nm.
McDowall, A.W., Smith, J.M., Dubochet, J.   +2 more
openaire   +5 more sources

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

Engineered protein subunit COVID19 vaccine is as immunogenic as nanoparticles in mouse and hamster models

Scientific Reports
Initial studies on the immunogenicity of SARS-CoV-2 (CoV-2) S glycoprotein (“spike”) as a protein subunit vaccine suggested sub-optimal efficacy in mammals.
Melissa M. Matthews, Tae Gyun Kim, Keon Young Kim, Vladimir Meshcheryakov, Higor Alves Iha, Miho Tamai, Daiki Sasaki, Paola Laurino, Saacnicteh Toledo-Patiño, Mary Collins, Tzung-Yang Hsieh, Satoshi Shibata, Noriko Shibata, Fumiko Obata, Jun Fujii, Toshihiro Ito, Hiroshi Ito, Hiroki Ishikawa, Matthias Wolf   +18 more
doaj   +1 more source