Results 61 to 70 of about 133,701 (285)

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Unsupervised cryo-EM data clustering through adaptively constrained K-means algorithm

, 2016
In single-particle cryo-electron microscopy (cryo-EM), K-means clustering algorithm is widely used in unsupervised 2D classification of projection images of biological macromolecules.
Mao, Youdong, Wu, Jiayi, Xu, Yaofang, Yin, Chang-Cheng   +3 more
core   +7 more sources

Microscale Fluid Behavior during Cryo-EM Sample Blotting [PDF]

, 2020
Blotting has been the standard technique for preparing aqueous samples for single-particle electron cryo-microscopy for over three decades. This technique removes the excess solution from a transmission electron microscope grid by pressing absorbent ...
Armstrong, Maxim, Fletcher, Daniel A., Glaeser, Robert M., Gomez, Salvador, Han, Bong-Gyoon, Turner, John   +5 more
core   +1 more source

Structure of a bacterial type IV secretion core complex at subnanometre resolution [PDF]

, 2013
Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4.
Chandran, Vidya, Fronzes, Remi, Laeremans, T., Lian, Pei W., Orlova, Elena, Pardon, E., Remaut, Han, Rivera-Calzada, A., Savva, Christos G., Steyaert, J., Waksman, Gabriel   +10 more
core   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Structural Insights into Cell Wall-Related Vesicle Secretion with Different Mechanisms

Plants
Exocytosis is a fundamental biological process in all eukaryotes involving the vesicular transport of cellular cargo to the plasma membrane or extracellular space.
Jiawen Yang, Sheng Chang, Linlin Li, Xiaoyu Ma, Hua Han, Zheng Liu, Jian Zhu   +6 more
doaj   +1 more source

The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes

Nature Communications, 2016
Continuous cycles of nucleo-cytoplasmic transport require disassembly of transport receptor-Ran-GTP complexes in the cytoplasm. Here the authors elucidate the specific function of the RanBP2 complex in the disassembly process.
Tobias Ritterhoff, Hrishikesh Das, Götz Hofhaus, Rasmus R. Schröder, Annette Flotho, Frauke Melchior   +5 more
doaj   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Cryo-Electron Microscopy for Cancer

International Journal of Trends in OncoScience, 2023
A review paper on cryo-electron microscopy (cryo-EM) is essential to assess the recent advancements in this revolutionary imaging technique. As cryo-EM continues to revolutionize structural biology, a comprehensive review can consolidate the knowledge, highlight technical challenges, and offer insights into future developments, promoting better ...
Dr Bablee Jyoti, Dr Anand Mohan Jha, Dr Jagadeesh Dhamodharan, Dr Aswinprakash Subramanian   +3 more
openaire   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source