Results 81 to 90 of about 2,439,094 (286)
Nature Communications, 2020 Microtubule (MT) organization is regulated by many microtubule-associated proteins (MAPs) that contain intrinsically disordered regions. Here authors produce [13C, 15N] labeled, functional microtubules from human cells for solid-state NMR which allows ...
Yanzhang Luo +8 more
doaj +1 more source
Cryo-electron microscopy shapes up [PDF]
Nature, 2018 As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are. As the imaging technique produces ever-sharper protein structures, researchers are racing to develop tools to assess how accurate they are.
openaire +2 more sources
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
FEBS Open Bio, EarlyView.Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
High-resolution cryo-electron microscopy study of structure anddynamics of yeast fatty acid synthase by single particle analysis [PDF]
, 2010 This thesis presents a 5.9 Å map of yeast FAS obtained by cryo-electron microscopy using single particle analysis (SPA). The EM-map has been analyzed both by quantitative and qualitative analysis to aid in understanding of the structure and dynamics of ...
Kumari, Preeti, Kumari, P.
core
FEBS Open Bio, EarlyView.This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli +2 more
wiley +1 more source
Cryo‐Electron Microscopy for Unveiling the Sensitive Battery Materials
Small Science, 2021 Deep chemical and structural investigation of battery components is increasingly imperative for exploring new electrode materials and their performance iterations for the next‐generation of energy storage devices with high energy density.
Zhijin Ju +7 more
doaj +1 more source
Advanced Functional Materials, EarlyView.A Li2O‐rich solid electrolyte interphase (SEI) is engineered on graphite through a propylene carbonate‐based electrolyte to spatially regulate Li plating. The homogeneous interphase lowers Li nucleation barriers, promotes conformal Li deposition around graphite particles, and suppresses dendritic accumulation, enabling durable hybrid Li‐ion/Li‐metal ...
Robert Kuphal +8 more
wiley +1 more source
Genome Medicine, 2018 Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs.
Praneeth Reddy Devulapally +7 more
doaj +1 more source
Combined approaches to flexible fitting and assessment in virus capsids undergoing conformational change [PDF]
, 2013 Fitting of atomic components into electron cryo-microscopy (cryoEM) density maps is routinely used to understand the structure and function of macromolecular machines.
Shakeel, S. +10 more
core +1 more source