Results 81 to 90 of about 133,701 (285)

Raman Microscopy at the Organic–Inorganic Interfaces in Human Calcified Aortic Valves Shows the Co‐Existence of Whitlockite Crystals and Carbonated Hydroxyapatite‐Mineralized Collagen Fibrils

Advanced Functional Materials, EarlyView.
This study advances our understanding of aortic valve stenosis by capturing spatially resolved chemical and structural changes at the nanoscale. The findings highlight the potential of combined Raman and electron microscopy for understanding calcification mechanisms across diverse tissue types.
Robin H. M. Van der Meijden, Carolien T. A Kuster, Amber van Broekhoven, Raphaela Allgayer, Luco Rutten, Rona Roverts, Marta Cerruti, Elena Macías‐Sánchez, Jan Hein Cornel, Salloua el Messaoudi, Anat Akiva, Nico Sommerdijk   +11 more
wiley   +1 more source

Dual function of a highly conserved bacteriophage tail completion protein essential for bacteriophage infectivity

Communications Biology
Infection of bacteria by phages is a complex multi-step process that includes specific recognition of the host cell, creation of a temporary breach in the host envelope, and ejection of viral DNA into the bacterial cytoplasm.
Isabelle Auzat, Malika Ouldali, Eric Jacquet, Beatrix Fauler, Thorsten Mielke, Paulo Tavares   +5 more
doaj   +1 more source

Cryo‐Electron Microscopy for Unveiling the Sensitive Battery Materials

Small Science, 2021
Deep chemical and structural investigation of battery components is increasingly imperative for exploring new electrode materials and their performance iterations for the next‐generation of energy storage devices with high energy density.
Zhijin Ju, Huadong Yuan, Ouwei Sheng, Tiefeng Liu, Jianwei Nai, Yao Wang, Yujing Liu, Xinyong Tao   +7 more
doaj   +1 more source

pH-dependent gating mechanism of the Helicobacter pylori urea channel revealed by cryo-EM. [PDF]

, 2019
The urea channel of Helicobacter pylori (HpUreI) is an ideal drug target for preventing gastric cancer but incomplete understanding of its gating mechanism has hampered development of inhibitors for the eradication of H. pylori. Here, we present the cryo-
Cui, Yanxiang, Munson, Keith, Sachs, George, Strugatsky, David, Wen, Yi, Zhou, Kang, Zhou, Z Hong   +6 more
core   +1 more source

Golgi apparatus analyzed by cryo-electron microscopy [PDF]

Histochemistry and Cell Biology, 2013
In 1898, the Golgi apparatus was discovered by light microscopy, and since the 1950s, the ultrastructure composition is known by electron microscopic investigation. The complex three-dimensional morphology fascinated researchers and was sometimes even the driving force to develop novel visualization techniques.
Han, H., Bouchet-Marquis, C., Hübinger, J., Grabenbauer, M.   +3 more
openaire   +3 more sources

3D‐Printed Porous Hydroxyapatite Formed via Enzymatic Mineralization

Advanced Functional Materials, EarlyView.
Bone combines lightness, strength, and the ability to heal, inspiring new materials design. This work introduces a room‐temperature, enzyme‐mediated 3D printing method to create porous hydroxyapatite scaffolds. The process avoids energy‐intensive sintering, preserves bioactivity, and allows control over porosity and mineralization.
Francesca Bono, Anna Puiggalí‐Jou, Greta Cocchi, Mariangela Miccoli, Katharina Maniura‐Weber, Marcy Zenobi‐Wong, Esther Amstad   +6 more
wiley   +1 more source

Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Genome Medicine, 2018
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs.
Praneeth Reddy Devulapally, Jörg Bürger, Thorsten Mielke, Zoltán Konthur, Hans Lehrach, Marie-Laure Yaspo, Jörn Glökler, Hans-Jörg Warnatz   +7 more
doaj   +1 more source

Liquid Phase Transmission Electron Microscopy: A Window into the Early Stages of Complex Material Formation

Advanced Functional Materials, EarlyView.
Liquid‐phase transmission electron microscopy enables direct observation of nucleation and growth processes in solution. This review is dedicated to the remembrance of Helmut Cölfen and highlights recent studies on complex materials—oxides, biominerals, organic–inorganic crystals—which were central to his research activity. It summarizes key milestones,
Charles Sidhoum, Vinavadini Ramnarain, Nathaly Ortiz Peña, Dris Ihiawakrim, Clément Sanchez, Ovidiu Ersen   +5 more
wiley   +1 more source

Data-Driven Tight Frame for Cryo-EM Image Denoising and Conformational Classification

, 2018
The cryo-electron microscope (cryo-EM) is increasingly popular these years. It helps to uncover the biological structures and functions of macromolecules. In this paper, we address image denoising problem in cryo-EM. Denoising the cryo-EM images can help
Cai, Jian-Feng, Gu, Hanlin, Huang, Xuhui, Wang, Wei, Wang, Yang, Xian, Yin, Yao, Yuan   +6 more
core   +1 more source

From Clinic to Computation: Multiscale Bioengineering Strategies for Durable Biological Aortic Valve Replacements

Advanced Functional Materials, EarlyView.
Bioprosthetic aortic valves have revolutionized the treatment of aortic stenosis, but their durability is limited by structural valve deterioration (SVD). This review focuses on the pericardial tissue at the heart of these valves, examining how its mechanical properties and calcification drive fatigue and failure.
Gabriele Greco, Pierfrancesco Gaziano, Serena Anglese, Simone Morganti, Carlo de Vincentiis, Ferdinando Auricchio, Michele Marino, Michele Conti   +7 more
wiley   +1 more source