Results 101 to 110 of about 2,439,094 (286)

Cryo-electron microscopy structure and densities of a pentameric ligand-gated ion channel GLIC

, 2023
This Dataset contains cryo-electron microscopy (cryo-EM) densities and a pdb model of a Gloeobacter violaceus ligand-gated ion channel (GLIC). The densities were generated with RELION data processing software and the model was built with COOT software ...
Urska Rovsnik (14437806), Erik Lindahl (7349455), Rebecca Howard (13969191), Cathrine Bergh (10313224)   +3 more
core   +1 more source

In Situ Interfacial Assembly of Dynamic Semi‐Interpenetrating Biopolymer Networks for Robust and Mechanically Adaptive Droplet Interfaces

Advanced Functional Materials, EarlyView.
A dynamic interfacial semi‐interpenetrating polymer network is constructed by sequential assembly of β‐sheet‐rich SF and CS‐RL. The SF framework provides mechanical strength, while dynamically associated CS‐RL enables local rearrangement, allowing droplets to rapidly reconfigure without loss of interfacial integrity.
Yaping Zhang, Yujie Liu, Yikai Xu, Xunda Feng, Ling Zhang, Hongbo Zeng, Hao Liu, Xiaohui Mao   +7 more
wiley   +1 more source

Cryo-electron microscopy image reconstruction data.

, 2013
Cryo-electron microscopy image reconstruction data.
James F. Conway (393058), Susan Hafenstein (204382), Jennifer L. Yoder (393054), Michael S. Carnegie (393055), Robert E. Ashley (393056), Alexander M. Makhov (393057), Kristin L. Shingler (393053)   +6 more
core   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

CryoEM screening with a blotting instrument: quantifying parameters affecting ice thickness using semi-automated image analysis

Methods in Microscopy
Cryo-electron microscopy (cryoEM) grid preparation is one of the bottlenecks in using cryoEM for macromolecular structure determination. Despite significant advancements in the development of blot-free instruments, their high cost limits their widespread
Valimehr Sepideh, Cho Ellie, Brown Hamish G., McMillan Paul J., Hanssen Eric   +4 more
doaj   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Global structural survey of the flagellotropic myophage φTE infecting agricultural pathogen Pectobacterium atrosepticum

Nature Communications
Bacteriophages offer a promising alternative to drug-based treatments due to their effectiveness and host specificity. This is particularly important in agriculture as a biocontrol agent of plant diseases.
James Hodgkinson-Bean, Rafael Ayala, Nadishka Jayawardena, Georgia L. Rutter, Bridget N. J. Watson, David Mayo-Muñoz, James Keal, Peter C. Fineran, Matthias Wolf, Mihnea Bostina   +9 more
doaj   +1 more source

Differential Sedimentation and Cryo‐TEM Mapping Resolve Structural Heterogeneity in Conjugated Polymer Nanowires for Programmable Stretchable Electronics

Advanced Materials, EarlyView.
Density‐driven differential sedimentation fractionates solution‐assembled P3HT nanowires into crystalline and disordered constituents. High‐resolution cryo‐TEM directly visualizes the molecular chain packing within individual nanowires in real space, and recombining the fractions programs the mobility–stretchability trade‐off without chemical ...
Junyeon Yoon, Jun Ho Hwang, Eunji Lee
wiley   +1 more source

New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography [PDF]

, 2017
Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample.
Gaechter, Leander, Sieckmann, F., Sonnen, A., Briggs, J., Bebeacua, C., Clarke, M., Clarke, Mairi, Bebeacua, Cecilia, Schorb, Martin, Schorb, M., Bykov, Y., Bykov, Yury S., Sonnen, Andreas F.-P., Lihl, Reinhard, Avinoam, Ori, Lihl, R., Avinoam, O., Gaechter, L., Sieckmann, Frank, Briggs, John A.G.   +19 more
core   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source