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Methanol as a cryoprotectant for equine embryos
Theriogenology, 2004Equine embryos (n=43) were recovered nonsurgically 7-8 days after ovulation and randomly assigned to be cryopreserved in one of two cryoprotectants: 48% (15M) methanol (n=22) or 10% (136 M) glycerol (n=21). Embryos (300-1000 microm) were measured at five intervals after exposure to glycerol (0, 2, 5, 10 and 15 min) or methanol (0, 15, 35, 75 and 10 min)
L D, Bass +5 more
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Biochemistry of Cryoprotectants
1991The role of polyhydric alcohols in cryoprotection is probably the most extensively studied feature of insect cold hardiness. The importance of glycerol as a cryoprotectant was first recognized by R. W. Salt after he and others linked the presence of high levels of glycerol with winter hibernation, diapause, or freezing survival (Salt, 1957, 1959, 1961;
Kenneth B. Storey, Janet M. Storey
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New cryoprotectant for cryorefractive surgery
Journal of Cataract and Refractive Surgery, 1991Cryorefractive surgeries, keratomileusis, keratophakia, and epikeratophakia cause destruction of keratocytes, which may result in postoperative corneal haze. We examined the effects of two cryoprotectants on keratocyte survival following freeze injury. We compared the ability of CPTES and the standard cryoprotectant KM-26 to prevent keratocyte death by
K, Kratz-Owens +2 more
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On the mechanism of cryoprotection by polyethylene oxide
Cryobiology, 1976Abstract Bone marrow cells and peripheral blood leukocytes have been exposed to a 15% solution of polyethylene oxide of molecular weight 400 (PEO-400) and the effect on DNA synthesis, oxygen uptake, and the activity of oxidative-reductive enzymes has been studied.
N S, Pushkar +2 more
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Analysis of the permeation of cryoprotectants in cartilage
Cryobiology, 2007Some tissues, such as cartilage and cornea, carry an internal fixed negative charge, leading to a swelling pressure that is balanced by tensile stress in the tissue matrix. During the addition and removal of cryoprotectants the changes in osmotic pressure will cause the tissue to deform.
Zhang, Shaozhi, David E, Pegg
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Resonance, 2002
Cold-adapted organisms are known to accumulate cryoprotective substances inside their cells. Glycine betaine is a bacterial cryoprotectant. It is believed to act by stabilizing cellular proteins and membranes at low temperature.
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Cold-adapted organisms are known to accumulate cryoprotective substances inside their cells. Glycine betaine is a bacterial cryoprotectant. It is believed to act by stabilizing cellular proteins and membranes at low temperature.
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Mechanisms of cryoprotection of cells by extracellular cryoprotection
Cryobiology, 1989openaire +1 more source