Acid Phosphatase in the Pathogenic and Nonpathogenic Hemoflagellates, Cryptobia spp., of Fishes
The Journal of Parasitology, 1996Acid phosphatase (ACP) was detected in whole-cell lysates, membrane-bound and water-soluble fractions of Cryptobia salmositica (pathogenic and nonpathogenic vaccine strains), Cryptobia bullocki, and Cryptobia catostomi using p-nitro-phenylphosphate as the substrate.
X, Zuo, P T, Woo
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Improved Culture Media for Piscine Hemoflagellates, Cryptobia and Trypanosoma (Kinetoplastida)
The Journal of Parasitology, 1998Increasing the Hepes buffer in minimum essential medium from 25 mM to 100 mM yielded a significantly larger number of Cryptobia salmositica. Cryptobia salmositica (pathogenic and nonpathogenic strains), Cryptobia bullocki, and Trypanosoma danilewskyi did not multiply either in heat-inactivated trout plasma (< or =25%) or in less than 10% fresh trout ...
B F, Ardelli, P T, Woo
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Transmission of the Hemoflagellate, Cryptobia salmositica Katz, 1951, by a Rhynchobdellid Vector
The Journal of Parasitology, 1965The transmission of Cryptobia salmositica Katz, 1951, a hemoflagellate of freshwater teleosts, was investigated in experiments involving the rhynchobdellid leech, Piscicola salmositica Meyer, 1946. Uninfected leeches developed metacyclic stages of Cryptobia in their digestive tracts after feeding on naturally infected torrent sculpins (Cottus rhotheus).
C D, BECKER, M, KATZ
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Characterization of a 200 kDa glycoprotein (Cs-gp200) on the pathogenic piscine haemoflagellate Cryptobia salmositica [PDF]
The 200 kDa antigenic doublet of the pathogenic haemoflagellate Cryptobia salmositica Katz, 1951, was detected using a monoclonal antibody (MAb-001) in 1-dimensional SDS-PAGE. This antigenic doublet is a glycoprotein since it was susceptible to both protease K and to sodium m-periodate oxidation and is designated Cs-gp200.
Ptk Woo, Patrick T K Woo
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The therapeutic use of isometamidium chloride against Cryptobia salmositica in rainbow trout Oncorhynchus mykiss [PDF]
Rainbow trout Oncorhynchus mykiss injected intramuscularly with isometamidium chloride (0.01 or 0.1 mg kg-1) at 3 wk post-infection and given a booster 2 wk later had significantly lower parasitaemias than infected controls. Packed cell volume increased after treatment and remained higher than in infected controls. The concentration of isometamidium in
B F Ardelli, Ptk Woo, Patrick T K Woo
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Cryptobia and Cryptobiosis in Fishes
1987Publisher Summary Cryptobia is a flagellate with two flagella (one being attached to the body), a prominent kinetoplast, and a nucleus. The parasite has been reported on the body surface, in the digestive tract, and in the blood of fishes. The chapter helps to unify and clarify the relationship among the various groups of cryptobia and, as a result ...
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In vitro Effects of Fetal Bovine Serum and Glucose on Multiplication of Cryptobia salmositica
The Journal of Parasitology, 1991Cryptobia salmositica multiplied rapidly at 10 C in a minimum essential medium (containing 1.0 mg glucose/ml, Hanks' salts and L-glutamine) supplemented with heat-inactivated fetal bovine serum (FBS) and HEPES buffer (25 mM). The multiplication rate of C.
S, Li, P T, Woo
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Cryptobia cataractae from the Blood of Semotilus atromaculatus: Structure and Division in the Fish
The Journal of Parasitology, 1990A cryptobiid was found in the blood of 2 of 9 Semotilus atromaculatus from a tributary of the Saugeen River in Ontario, Canada. Blood inoculation produced an infection in 2 uninfected S. atromaculatus but not in any Oncorhynchus mykiss, Catostomus commersoni, or Carassius auratus.
S R, Jones, P T, Woo
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Using the sealed jar technique (also called residual oxygen bioassay), rainbow trout fry infected with Cryptobia salmositica were more susceptible than non-infected fish to environmental hypoxia. The Winkler technique (azide modification) was used to determine the residual dissolved oxygen in the water. Susceptibility of infected fish increased with 1)
P T, Woo, S D, Wehnert
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We report on the identification of a Cryptobia genomic DNA gene, predict it to encode a S-adenosylmethionine synthetase signature 1 motif and propose to name it S-adenosylmethionine synthetase (MAT). The open reading frame of MAT is 1,046 bp with 341 deduced amino acids.
Palmy R, Jesudhasan, Patrick T K, Woo
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