Results 121 to 130 of about 6,208,210 (311)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Comparative Performance of Tetraphosphonate and Diphosphonate as Reverse Osmosis Scale Inhibitors
Reverse osmosis (RO) technologies are widely used throughout the world for drinking, industrial and waste water treatment purposes. Proper and economically attractive operation of RO installations should be provided by the correct maintenance of all ...
Andrianov Alexey +4 more
doaj +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Crystal Growth of Salicylic Acid in Organic Solvents
The crystal growth rate of salicylic acid has been determined by seeded isothermal desupersaturation experiments in different organic solvents (methanol, acetone, ethyl acetate, and acetonitrile) and at different temperatures (10, 15, 20, and 25 °C).
Michael Svärd +8 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Post-breakage crystal growth is obscure. Recently, form I paracetamol (PCM) crystals, broken to expose their internal cleavage plane (010), grew back into their original shapes through “regeneration.” This work reports on regeneration in crystals with ...
Etit, Deniz +3 more
core +1 more source
Incorporation and effects of impurities in different growth zones within basic ammonothermal GaN
The ammonothermal method is one of the most promising candidates for large-scale bulk GaN growth due to its scalability and high crystalline quality. However, emphasis needs to be put on understanding the incorporation and effects of impurities during ...
Speck, James S. +7 more
core +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Crystal growth part i. background to crystal growth
A programme of crystal growing has been in progress in the Mineral Sciences Division of the Mines Branch, as part of a Sulphide Research Programme, during the past six years. During this time, the growth of crystals of about fifty materials has been attempted in the field of sulphides, arsenides and sulpharsenides of Fe, Co, Ni, Zn and Cu, and with ...
openaire +1 more source

