Results 151 to 160 of about 3,801 (175)

The classic swine fever virus (CSFV) core protein can enhance de novo-initiated RNA synthesis by the CSFV polymerase NS5B

Virus Genes, 2014
Study of the classical swine fever virus (CSFV) replication is challenging because it is a BSL-3-Ag pathogen that requires specialized facilities. We developed a cell-based assay in human embryonic kidney 293T cells that can quantify the activities of NS5B, the CSFV RNA-dependent RNA polymerase.
Weiwei, Li, Yanming, Zhang, C Cheng, Kao
openaire   +2 more sources

Characterization of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) helicase activity and its modulation by CSFV RNA-dependent RNA polymerase

Virus Research, 2009
Classical swine fever virus (CSFV) nonstructural protein 3 (NS3) is believed to possess three enzyme activities that are likely to be essential for virus replication: a serine protease located in the N-terminus and NTPase as well as helicase activities located in the C-terminus. In this report, we expressed NS3 helicase domain (NS3h) in E.
Guoyuan, Wen, Jinnuo, Xue, Yanping, Shen, Chuyu, Zhang, Zishu, Pan   +4 more
openaire   +2 more sources

A strategy for detecting CSFV using DNAzyme-HCR cascade amplification

Analytical Methods
The Hybridization Chain Reaction (HCR) is an isothermal amplification technique widely used for sensing nucleic acids and small molecules.
Xiuen Cao, Jiajing Cai, Zhilin He, Haofei Ji, Ruowei Sun, Xun Zhang, Chuanpin Chen, Qubo Zhu   +7 more
openaire   +2 more sources

The CSFV DNAChip: A novel diagnostic assay for classical swine fever virus

Journal of Virological Methods, 2014
A novel assay, the CSFV DNAChip, was developed to clearly and rapidly discriminate three genotypes of classical swine fever virus (CSFV). Total RNA was extracted from clinical samples and then subjected to a one-step reverse-transcription polymerase chain reaction (RT-PCR) using Cy3-labeled primers from the 5' non-coding region (NCR) of CSFV. Amplicons
Yong Kwan, Kim, Seong-In, Lim, Yoon-Young, Cho, Jae-Young, Song, JoonBae, Kim, Dong-Jun, An   +5 more
openaire   +2 more sources

Chimeric (marker) C-strain viruses induce clinical protection against virulent classical swine fever virus (CSFV) and reduce transmission of CSFV between vaccinated pigs

Vaccine, 2001
Two live recombinant vaccines (Flc9 and Flc11) against classical swine fever (CSF) were evaluated for their capacity to reduce transmission of virulent CSF virus (CSFV) among vaccinated pigs. In Flc9 the 5' terminal half of the E2 gene of the C-strain, a CSFV vaccine strain, was exchanged with the homologous gene of the bovine viral diarrhoea virus ...
de Smit, A.J., Bouma, A., van Gennip, H.G., de Kluijver, E.P., Moormann, R.J.   +4 more
openaire   +2 more sources

Apoptosis of lymphoid tissues after classical swine fever virus (CSFV) inoculation in CSFV-vaccinated piglets

A study on the immune response and apoptosis of lymphoid tissues in CSFV-vaccinated pig was performed. The pigs from CSF-free herd 2 years before study were classified into 3 groups, 6 pigs of each; group A and B had average matermal antibody (Ab) ≥ 64 in group A and ≤ 8 in group B and they were vaccinated at 3 week old; group C which had matermal Ab ...
openaire   +1 more source

Major vault protein inhibits CSFV replication by promoting cell apoptosis

Veterinary Microbiology
Classical swine fever virus (CSFV) remains a major pathogen posing substantial threats to the global swine industry. CSFV infection modulates the expression of numerous host cell proteins, with certain factors facilitating viral replication while others exert inhibitory effects.
Bihao Luo, Na Su, Yuehan Quan, Ning Li, Chen Chen, Liang Zhang, Lei Xu, Kangkang Guo   +7 more
openaire   +2 more sources

ANXA2 stabilizes mTOR at the plasma membrane to facilitate autophagic flux for CSFV release

Veterinary Microbiology
Classical swine fever virus (CSFV), a member of the Flaviviridae family, remains a major pathogen responsible for substantial economic losses in the global swine industry. Autophagy plays a critical role in the life cycle and virulence of CSFV, however, the mechanisms through which the virus regulates autophagy are still not fully understood.
Tao, Wang, Liangcai, Da, Junfang, Zhao, Hong, Yuan, Ying, Sun, Liang, Zhang, Kun, Li, Jing, Zhang, Pu, Sun, Zhixun, Zhao, Qiang, Zhang, Yuanji, Zhang, Yebing, Liu, Xingwen, Bai, Zengjun, Lu   +14 more
openaire   +2 more sources

Induction of autophagy and suppression of type I IFN secretion by CSFV

Autophagy, 2021
Mingqiu Zhao, Keke Wu, Jinding Chen
exaly  

IL-1β induced by PRRSV co-infection inhibited CSFV C-strain proliferation via the TLR4/NF-κB/MAPK pathways and the NLRP3 inflammasome

Veterinary Microbiology, 2022
Lei Zhou
exaly