Structural insights into <i>Escherichia coli</i> CsgA amyloid fibril assembly revisited. [PDF]
mBioABSTRACT Curli are a widespread component of proteobacterial biofilms. They are functional amyloid fibers that aid in structuring the extracellular matrix that surrounds these multicellular communities. As a functional amyloid, they are the product of a dedicated assembly pathway that needs to navigate the ...
Sleutel M, Remaut H.
europepmc +5 more sources
Reply to Sleutel and Remaut, "Structural insights into Escherichia coli CsgA amyloid fibril assembly revisited". [PDF]
mBioBu F, Dee DR, Liu B.
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The bacterial chaperone CsgC inhibits functional amyloid CsgA formation by promoting the intrinsically disordered pre-nuclear state. [PDF]
J Biol ChemAbstractE. coliassembles a functional amyloid called curli during biofilm formation. The major curlin subunit is the CsgA protein, which adopts a beta-sheet rich fold upon fibrillization. The chaperone-like protein CsgC inhibits CsgA amyloid formation.
Balistreri A +10 more
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CsgA gatekeeper residues control nucleation but not stability of functional amyloid. [PDF]
Protein SciAbstractFunctional amyloids, beneficial to the organism producing them, are found throughout life, from bacteria to humans. While disease‐related amyloids form by uncontrolled aggregation, the fibrillation of functional amyloid is regulated by complex cellular machinery and optimized sequences, including so‐called gatekeeper residues such as Asp ...
Olsen WP +7 more
europepmc +6 more sources
Force spectra of single bacterial amyloid CsgA nanofibers
RSC Advances, 2020 Magnetic tweezers were used to study the force spectra of CsgA, a major protein subunit of Escherichia coli biofilms, at fibril level.
Jingqi Lv +8 more
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Relationship between flu and csgA Virulence Genes and Biofilm Production in Uropathogenic Escherichia coli [PDF]
Journal of Advanced Biomedical Sciences, 2020 Background & Objective: There are a variety of virulence factors that allow Uropathogenic E. coli (UPEC) to cause infection in the urinary tract among which biofilm production ability plays the most important role.
Faezeh Moghadas Kasani +2 more
doaj +1 more source
CSGA: A DUAL POPULATION GENETIC ALGORITHM BASED ON MEXICAN CAVEFISH GENETIC DIVERSITY
Jordanian Journal of Computers and Information TechnologyGenetic algorithms (GAs) are search algorithms based on population genetics and natural selection concepts. Maintaining population variety in GAs is critical for ensuring global exploration and mitigating the risks of premature convergence.
Esra'a Alkafaween +3 more
doaj +2 more sources
csgA expression entrains Myxococcus xanthus development. [PDF]
Genes & Development, 1992 The development cycle of the myxobacterium Myxococcus xanthus consists of three partially overlapping morphological stages referred to as rippling, fruiting body formation, and sporulation, all of which are absent in csgA null mutants. The CsgA gene product is an extracellular protein, referred to as the C signal, which is essential for developmental ...
S. Li +2 more
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Comprehensive Analysis of blaCTX-M1 Gene Expression Alongside iutA, csgA, and kpsMII Virulence Genes in Septicemic Escherichia coli Using Real-Time PCR [PDF]
MicroorganismsBackground: Sepsis is a serious worldwide health concern, and Escherichia coli (E. coli) is the main cause. This study investigates the co-expression of blaCTX-M1 and iutA, csgA, and kpsMII genes in E.
Mohsen Karbalaei +3 more
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Dimerization energetics of curli fiber subunits CsgA and CsgB [PDF]
npj Computational Materials, 2019 AbstractCurli fibers are functional amyloids that exhibit strong adhesion and robust self-assembly as biofilm structural components; however, the binding energetics and mechanical properties of wild-type curli are not well understood. To address this, we present dimer structures made up of the major and minor curli subunits (CsgA and CsgB), perform ...
Martha Dunbar +2 more
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