Results 171 to 180 of about 538,969 (317)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Beyond cultural competence: online training for healthcare professionals in refugee healthcare; a quasi-experimental mixed-methods study protocol. [PDF]

MethodsX
Koutsouradi G, Dimitrakaki C, Giannakopoulos G, Riza E, Lagiou A, Kolaitis G, Benetou V.   +6 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Pathways to healing: balancing the tensions between harm reduction and cultural practices in a managed alcohol program for Indigenous adults in Calgary, Canada. [PDF]

Front Public Health
McLean A, Lucas K, Chief Moon J, Prairie Chicken A, Pauly B, Nixon L, Lamping S, Robinson J, Brown M, Wan-Sai-Cheong L, Zaretsky L, Morin T, Milaney K.   +12 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

MEPT-LLM: a multimodal generative AI model for identifying and understanding cultural-emotional barriers in the language classroom. [PDF]

Front Psychol
Bai X, Bai M.
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Diverse strategies for diverse carers: the cultural context of family carers in NSW

, 2007
This study presents the findings of cultural research into the everyday experiences of carers from culturally and linguistically diverse (CALD) and Anglo-Australian backgrounds.
Sharon Chalmers, Beatriz Cardona
core  

The influence of parents gender attitudes on students career choices: a sociocultural analysis. [PDF]

Front Sociol
Zharkynbek A, Serikkhan Z, Makhpal S, Aigul A, Sergazy K.   +4 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source