Understanding and measuring mechanical signals in the tumor stroma
This review discusses cancer‐associated fibroblast subtypes and their functions, particularly in relation to extracellular matrix production, as well as the development of 3D models to study tumor stroma mechanics in vitro. Several quantitative techniques to measure tissue mechanical properties are also described, to emphasize the diagnostic and ...
Fàtima de la Jara Ortiz+3 more
wiley +1 more source
Non-steroidal anti-inflammatory drugs (NSAIDs) regimens enhance synergistic selective anticancer efficacy of chemotherapeutic agents on cultured cells. [PDF]
Chou MY, Lin-Shiau SY.
europepmc +1 more source
Fiji-Based Tool for Rapid and Unbiased Analysis of SA-β-Gal Activity in Cultured Cells. [PDF]
Krzystyniak A+3 more
europepmc +1 more source
REVERSIBLE DEGRADATION OF POLYRIBOSOMES IN CHANG CELLS CULTURED IN A GLUTAMINE-DEFICIENT MEDIUM [PDF]
Eva Eliasson, G. Eric Bauer, T. Hultin
openalex +1 more source
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques+5 more
wiley +1 more source
The clinical spectrum of SMA‐PME and in vitro normalization of its cellular ceramide profile
Abstract Objective The objectives of this study were to define the clinical and biochemical spectrum of spinal muscular atrophy with progressive myoclonic epilepsy (SMA‐PME) and to determine if aberrant cellular ceramide accumulation could be normalized by enzyme replacement.
Michelle M. Lee+16 more
wiley +1 more source
Bio-orthogonal Glycan Imaging of Cultured Cells and Whole Animal <i>C. elegans</i> with Expansion Microscopy. [PDF]
Kuo JC+10 more
europepmc +1 more source
Assessment of protein inclusions in cultured cells using automated image analysis. [PDF]
McAlary L+6 more
europepmc +1 more source
Vitamin A and the biosynthesis of sulphated mucopolysaccharides. Experiments with rats and cultured neoplastic mast cells [PDF]
D. B. Thomas, C. A. Pasternak
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Novel mAbs could detect cancer‐associated membrane proteins on various type of pancreatic ductal adenocarcinomas (PDAC) by flow cytometry with viable PDAC cells and by immunohistochemistry with PDAC tissues. Results of protein expression were substantiated by mRNA expression by The Cancer Genome Atlas.
Takashi Nakano+16 more
wiley +1 more source