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Introduction to Cell Culture [PDF]

open access: possible, 2011
The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture.
Ragai R. Mitry   +3 more
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Sustained cell culture

Experimental Cell Research, 1957
Abstract Gas exchange, introduction of nutrients, and removal of waste products can be regulated in a new device for continuous sustained culture of cells in suspension. Strain L, clone 929, fibroblasts; HeLa cells; and strains KB, and Chang, liver cells were successfully propagated in this device.
K.S. McCarty, S. Graff
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Basic Cell Culture

2003
This article will describe the basic techniques required for successful cell culture. It will also act to introduce some of the other chapters in this volume. It is not intended, as this volume is not, to describe the establishment of a tissue culture laboratory, nor to provide a historical or theoretical survey of cell culture. There are several books
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Renal cell culture

Journal of Electron Microscopy Technique, 1988
AbstractMethods for the establishment and growth of renal cell types in culture are reviewed, with emphasis on current trends. General techniques available for the isolation and culture of glomerular cells have progressed from explant to enzyme dissociation and cloning techniques.
Jeffrey I. Kreisberg   +3 more
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Microfluidic cell culture

Current Opinion in Biotechnology, 2014
Microfluidic techniques allow precise control of fluids and particles at the nanoliter scale and facilitate simultaneous manipulation and analysis of cultured cells, starting from a single cell to larger populations and to intact tissues. The use of integrated microfluidic devices has considerably advanced the fields of quantitative and systems biology.
Mehling, Matthias, Tay, Savaş
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Apoptosis in cell culture

Current Opinion in Biotechnology, 1998
Most cells can exhibit a biochemical pathway which mediates their own destruction in a highly controlled and genetically defined manner. In animal cells, a morphologically distinct form of this 'programmed cell death' has been identified and extensively characterised. This phenomenon, which has been named apoptosis, accounts for most of the cell deaths
R. P. Singh, Mohamed Al-Rubeai
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Urothelial Cell Culture [PDF]

open access: possible, 2013
This chapter reviews the use of urothelial cells as a means to enhance tissue regeneration and wound healing in urinary tract system. It addresses the properties of urothelial cells, including their role as a permeability barrier to protect underlying muscle tissue from the caustic effects of urine and as one of the main cell types, along with smooth ...
Yuanyuan Zhang, Anthony Atala
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The Culture of Cells

Nature, 1959
Cell and Tissue Culture By Dr. John Paul. Pp. viii + 261 + 9 plates. (Edinburgh and London: E. and S. Livingstone, Ltd., 1959.) 30s. net.
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Cells in Culture

2010
Various cell types including stem cells, epithelial, neuronal and mesenchymal cells as well as different tumour cell types can be grown in vitro in tissue culture dishes or bottles. Routinely, plastic tissue culture dishes are used and cells can survive and multiply when supplied with appropriate culture medium, temperature and atmosphere.
Jürgen Roth, Margit Pavelka
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Thyroid Cells in Culture

1979
Publisher Summary Thyroid cell system consists of continuously growing epithelial cells derived from either experimentally induced, transplantable rat tumors of thyroid origin or normal rat thyroids. The thyroid is a peculiar endocrine gland whose main cellular type, the follicular cell, possesses unique morphological and functional properties.
Hayden G. Coon   +1 more
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