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Hematopoietic cell culture therapies (Part I): cell culture considerations

Trends in Biotechnology, 1996
Hematopoietic cell culture, or ex vivo expansion of hematopoietic cells, is a rapidly growing area of tissue engineering with many potential applications in bone-marrow transplantation, gene therapy and the production of blood products. Hematopoietic cultures are considerably more complex than established animal cell culture technologies owing to the ...
T A, McAdams   +2 more
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Cell Culture Contamination

2011
Microbial contamination is a major issue in cell culture, but there are a range of procedures which can be adopted to prevent or eliminate contamination. Contamination may arise from the operator and the laboratory environment, from other cells used in the laboratory, and from reagents.
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HEV Cell Culture

2016
Cell culture is an important research method in virology. Although many attempts have been conducted to culture HEV in cells, only a few cell culture systems were considered to be efficient enough for usage. Concentration of virus stocks, host cells, and medium components affects the culture efficiency and the genetic mutations during HEV passage were ...
Feng, Zhang, Youchun, Wang
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Microcarrier Cell Culture

Biotechnology and Genetic Engineering Reviews, 1988
(1988). Microcarrier Cell Culture. Biotechnology and Genetic Engineering Reviews: Vol. 6, No. 1, pp. 404-439.
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Amniotic Epithelial Cell Culture

2018
Ovine amniotic epithelial cells (oAEC) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, oAEC are object of intense study for regenerative medicine thanks to the several advantages in developing pre-clinical studies on a high value translational animal model, such as sheep. For this reason, a critical
Angelo Canciello   +3 more
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Plant Cell Suspension Cultures

2012
Plant cell suspension cultures are widely used in plant biology as a convenient tool for the investigation of a wide range of phenomena, bypassing the structural complexity of the plant organism in toto. The homogeneity of an in vitro cell population, the large availability of material, the high rate of cell growth and the good reproducibility of ...
MOSCATIELLO, ROBERTO   +2 more
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Thymic epithelial cell culture

Microscopy Research and Technique, 1997
Culture of epithelial cells from the thymus of children and laboratory animals has been used for more than two decades to evaluate both the nature of these cells and their importance in the selection and maturation of functional T cells. Especially by the use of serum-free cultures and by establishment of cell lines from cultured thymic epithelial ...
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Basic Cell Culture

2003
This article will describe the basic techniques required for successful cell culture. It will also act to introduce some of the other chapters in this volume. It is not intended, as this volume is not, to describe the establishment of a tissue culture laboratory, nor to provide a historical or theoretical survey of cell culture. There are several books
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Cell culture

1999
Abstract The ability to culture cells in vitro has enabled enormous advances to be made in biology, particularly in virology. Many cell types can be routinely pro liferated and cryopreserved, allowing consistent, characterized, contaminant free cells to be used both as a research and diagnostic tool.
K Blake, A Stacey
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Mesangial cell cultures.

Journal of nephrology, 2001
Cell culture of glomerular mesangial cells (MC) has been available to most renal research laboratories since the early 80s. Key to a large number of studies on the biochemistry and molecular biology of the glomerulus, MC in culture have extensive analogies with this in vivo rather undifferentiated intercapillary cell population.
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