Results 1 to 10 of about 2,772 (172)

Laser-assisted microbial culturomics [PDF]

Nature Communications
Even though metagenomics have revolutionized the characterization of the human microbiome, detailed mechanistic studies are impracticable, as there is a dearth of robust culture collections.
Taoran Qu, Lothar Koch, Rumjhum Mukherjee, Yilin Tu, Amy L. Seidel, Lisan D. Püttmann, Andreas Winkel, Ines Yang, Jasmin Grischke, Dejia Liu, Willem F. Wolkers, Sophie Kittler, Boris Chichkov, Meike Stiesch, Szymon P. Szafrański   +14 more
doaj   +4 more sources

Dereplication-assisted culturomics enables strain-level ecological analysis of the human gut microbiome [PDF]

Gut Microbes
Recent advances in culturomics have enabled large-scale recovery of microbial isolates from the human gut, generating extensive culture collections that bridge metagenomic predictions and experimental validation.
Soyoung Yeo, Hyunjoon Park
doaj   +2 more sources

Altitude-Associated Divergence of the Gut Microbiome in Endangered Forest Musk Deer: Evidence From Integrated Metagenomics, Metabolomics, and Culturomics. [PDF]

Evol Appl
ABSTRACT High‐altitude environments expose mammals and their gut symbionts to multifaceted stressors—hypoxia, cold, and intense UV radiation. Whether gut microbial communities undergo compositional restructuring in response to these stressors, and whether such restructuring carries translational value for captive conservation, remain unresolved ...
Huang F, Zhang Z, Zhao Y, Ye S, Gan M, Li X, Zhang Y, Chen L, Zhang Y, Chen L, Wang T, Huang J, Zhang X.   +12 more
europepmc   +2 more sources

Intratumoral <i>Enterobacter hormaechei</i> drives gemcitabine resistance in pancreatic cancer via <i>cdd</i> _L-mediated drug inactivation. [PDF]

Imeta
Gemcitabine resistance poses a critical barrier to improving survival in pancreatic cancer, yet the microbial drivers remain elusive. By integrating 16S rRNA amplicon sequencing with large‐scale culturomics across 114 clinical samples, we identified Enterobacter hormaechei as a key intratumoral pathogen. We demonstrate that E.
Peng JF, Li M, Niu T, Li J, Niu W, Zheng M, Ji Q, Li C, Shao C, Ding K.   +9 more
europepmc   +2 more sources

Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study

Pathogens, 2020
Background: In recent years, culture-independent molecular techniques have been developed to investigate microbiota considered uncultivable. However, the data in the literature suggest that molecular techniques and cultural methods target different ...
Romeo Patini, Luca Masucci, Gianluca Quaranta   +2 more
exaly   +3 more sources

Thomasclavelia ramosa and alcohol-related hepatocellular carcinoma: a microbial culturomics study [PDF]

Gut Pathogens
Background Gut microbiota alteration is implicated in the pathogenesis of alcoholic liver disease (ALD) and associated hepatocellular carcinoma (HCC). No study has characterized the dysbiosis associated with ALD by microbial culturomics, which certifies ...
Reham Magdy Wasfy, Anissa Abdoulaye, Patrick Borentain, Babacar Mbaye, Maryam Tidjani Alou, Aurelia Caputo, Claudia Andrieu, Giovanna Mottola, Anthony Levasseur, Matthieu Million, Rene Gerolami   +10 more
doaj   +2 more sources

Coupling culturomics and metagenomics sequencing to characterize the gut microbiome of patients with cancer treated with immune checkpoint inhibitors [PDF]

Gut Pathogens
Background The gut microbiome represents a novel biomarker for melanoma and non-small cell lung cancer (NSCLC) patients treated with immune checkpoint inhibitors (ICI).
Khoudia Diop, Babacar Mbaye, Somayeh Nili, Alysé Filin, Myriam Benlaifaoui, Julie Malo, Anne Sophie Renaud, Wiam Belkaid, Sebastian Hunter, Meriem Messaoudene, Karla A. Lee, Arielle Elkrief, Bertrand Routy   +12 more
doaj   +2 more sources

An optimized culturomics strategy for isolation of human milk microbiota

Frontiers in Microbiology
Viable microorganisms and a diverse microbial ecosystem found in human milk play a crucial role in promoting healthy immune system and shaping the microbial community in the infant’s gut.
Shanshan He
exaly   +3 more sources

Bacterial profiling of colorectal cancer biopsies: a culture-based study in Indian patients [PDF]

Frontiers in Cellular and Infection Microbiology
Emerging research has highlighted the significant role of microorganisms in the initiation and progression of colorectal cancer (CRC). However, further investigation is required to elucidate the precise mechanisms by which the microbial community or ...
Manish Kushwaha, Manish Kushwaha, Shubham Chaudhary, Akhilesh Kumar Singh, Govind K. Makharia, Anil Kumar   +5 more
doaj   +2 more sources

Progress in Culturemics Research on Beneficial Intestinal Bacteria [PDF]

Shipin Kexue, 2023
Gut microbes especially the beneficial ones play an important role in maintaining human health. At present, the research methods for intestinal microbes are mainly based on non-culture technologies such as metagenomics.
YU Xia, ZHAO Feiyan, SUN Zhihong
doaj   +1 more source