Results 41 to 50 of about 2,385 (168)
5‐Keto‐4‐deoxy glucarate dehydratase and 2‐keto‐3‐deoxy‐L‐arabinonate dehydratase share many similarities like structure, reaction mechanism, and their generated product, but exhibit very high substrate specificity. Here, we explore the evolutionary development of this specificity in the context of the enzyme superfamily and attempt to find promiscuous
Kathrin Hörnschemeyer +4 more
wiley +1 more source
Background A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO2) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as
Soyoung Kim +6 more
doaj +1 more source
Alliinase: A Comprehensive Review of the Pivotal Enzyme in Garlic Chemistry and Its Applications
Garlic crude extract was purified to obtain alliinase, which catalyzes alliin hydrolysis to release allicin. The free and immobilized alliinase shows potential applications in food, biotechnology, pharmaceuticals, health products, and biosensors. ABSTRACT Alliinase is a pyridoxal 5′‐phosphate (PLP)‐dependent lyase that serves as the cornerstone of the ...
Zhenlin Han +5 more
wiley +1 more source
: Brazil is the world’s largest producer of orange and passion fruit, which are destined mainly for industrialization, generating grand volumes of wastes.
GABRIEL O. LOCATELLI +2 more
doaj +1 more source
Abstract The Qualified Presumption of Safety (QPS) process was developed to provide a harmonised safety assessment approach to support EFSA Scientific Panels and Units. The QPS approach assesses the taxonomic identity, body of knowledge and safety of microorganisms intentionally added to the food and feed chain.
EFSA Panel on Biological Hazards (BIOHAZ) +26 more
wiley +1 more source
The air-inactivation of formate dehydrogenase FdsDABG from Cupriavidus necator
The nature of air-inactivation of the formate dehydrogenase FdsDABG from Cupriavidus necator has been investigated. It is found that superoxide, generated in the reaction of reduced enzyme with oxygen, is responsible for the loss of activity and that superoxide dismutase protects the enzyme from air-inactivation.
Hakopian, Sheron +2 more
openaire +4 more sources
Graphical Abstract and Lay Summary Cupriavidus necator NCIMB 11599 was cultivated with a fully automated pH–pO2‑dependent feeding strategy that enables controlled levulinic acid delivery and robust production of PHBVV terpolymer. The process was successfully transferred from 7.5 to 42‑L stirred‑tank reactors, maintaining PHA contents of ∼75% CDW and ...
Birk Achenbach +4 more
wiley +1 more source
Here a multiple heavy metal and antibiotic resistant bacterium Cupriavidus necator C39 (C. necator C39) was isolated from a Gold-Copper mine in Zijin, Fujian, China. C. necator C39 was able to tolerate intermediate concentrations of heavy metal(loid)s in
Zhenchen Xie +7 more
doaj +1 more source
Significance Statement Life on Earth depends on photosynthetic CO2 fixation via the Calvin–Benson–Bassham cycle to form organic carbon. This process evolved first in cyanobacteria and was later conveyed to eukaryotes, giving rise to plastids in algae and plants. To cope with low atmospheric CO2 concentrations that developed over the course of evolution,
Erik Zimmer +3 more
wiley +1 more source
Engineering a Synthetic Formatotrophic Yeast With Enhanced Growth Capacity on Formate
Engineering Yeast for Growth on Formate and Methanol via Serine‐Centered C1 Coupling. By engineering serine metabolism and remodeling the rGly pathway, we rendered yeast growth strictly dependent on formate. This synthetic formatotrophy enables growth on dual‐C1 substrates by channeling formate into the central metabolism via serine deaminases and ...
Shuyuan Guo +11 more
wiley +1 more source

