Results 41 to 50 of about 2,385 (168)

Investigation of Substrate Specificity in 5‐Keto‐4‐Deoxy‐Glucarate Dehydratase and 2‐Keto‐3‐Deoxy‐L‐Arabinonate Dehydratase

open access: yesChemBioChem, Volume 27, Issue 14, 29 July 2026.
5‐Keto‐4‐deoxy glucarate dehydratase and 2‐keto‐3‐deoxy‐L‐arabinonate dehydratase share many similarities like structure, reaction mechanism, and their generated product, but exhibit very high substrate specificity. Here, we explore the evolutionary development of this specificity in the context of the enzyme superfamily and attempt to find promiscuous
Kathrin Hörnschemeyer   +4 more
wiley   +1 more source

Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO2

open access: yesMicrobial Cell Factories, 2022
Background A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO2) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as
Soyoung Kim   +6 more
doaj   +1 more source

Alliinase: A Comprehensive Review of the Pivotal Enzyme in Garlic Chemistry and Its Applications

open access: yesFood Frontiers, Volume 7, Issue 4, July 2026.
Garlic crude extract was purified to obtain alliinase, which catalyzes alliin hydrolysis to release allicin. The free and immobilized alliinase shows potential applications in food, biotechnology, pharmaceuticals, health products, and biosensors. ABSTRACT Alliinase is a pyridoxal 5′‐phosphate (PLP)‐dependent lyase that serves as the cornerstone of the ...
Zhenlin Han   +5 more
wiley   +1 more source

Orange and Passion Fruit Wastes Characterization, Substrate Hydrolysis and Cell Growth of Cupriavidus necator, as Proposal to Converting of Residues in High Value Added Product

open access: yesAnais da Academia Brasileira de Ciências, 2019
: Brazil is the world’s largest producer of orange and passion fruit, which are destined mainly for industrialization, generating grand volumes of wastes.
GABRIEL O. LOCATELLI   +2 more
doaj   +1 more source

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 24: Suitability of taxonomic units notified to EFSA until March 2026

open access: yesEFSA Journal, Volume 24, Issue 7, July 2026.
Abstract The Qualified Presumption of Safety (QPS) process was developed to provide a harmonised safety assessment approach to support EFSA Scientific Panels and Units. The QPS approach assesses the taxonomic identity, body of knowledge and safety of microorganisms intentionally added to the food and feed chain.
EFSA Panel on Biological Hazards (BIOHAZ)   +26 more
wiley   +1 more source

The air-inactivation of formate dehydrogenase FdsDABG from Cupriavidus necator

open access: yesJournal of Inorganic Biochemistry, 2022
The nature of air-inactivation of the formate dehydrogenase FdsDABG from Cupriavidus necator has been investigated. It is found that superoxide, generated in the reaction of reduced enzyme with oxygen, is responsible for the loss of activity and that superoxide dismutase protects the enzyme from air-inactivation.
Hakopian, Sheron   +2 more
openaire   +4 more sources

Controlled Feeding Facilitates the Development of a Robust Production Process for Terpolymeric Polyhydroxyalkanoates

open access: yesBiotechnology Journal, Volume 21, Issue 6, June 2026.
Graphical Abstract and Lay Summary Cupriavidus necator NCIMB 11599 was cultivated with a fully automated pH–pO2‑dependent feeding strategy that enables controlled levulinic acid delivery and robust production of PHBVV terpolymer. The process was successfully transferred from 7.5 to 42‑L stirred‑tank reactors, maintaining PHA contents of ∼75% CDW and ...
Birk Achenbach   +4 more
wiley   +1 more source

Whole Genome Sequence Analysis of Cupriavidus necator C39, a Multiple Heavy Metal(loid) and Antibiotic Resistant Bacterium Isolated from a Gold/Copper Mine

open access: yesMicroorganisms, 2023
Here a multiple heavy metal and antibiotic resistant bacterium Cupriavidus necator C39 (C. necator C39) was isolated from a Gold-Copper mine in Zijin, Fujian, China. C. necator C39 was able to tolerate intermediate concentrations of heavy metal(loid)s in
Zhenchen Xie   +7 more
doaj   +1 more source

Functional and structural insights into cyanobacterial CO2 concentrating mechanisms: from compartmentalization to regulation

open access: yesThe Plant Journal, Volume 126, Issue 5, June 2026.
Significance Statement Life on Earth depends on photosynthetic CO2 fixation via the Calvin–Benson–Bassham cycle to form organic carbon. This process evolved first in cyanobacteria and was later conveyed to eukaryotes, giving rise to plastids in algae and plants. To cope with low atmospheric CO2 concentrations that developed over the course of evolution,
Erik Zimmer   +3 more
wiley   +1 more source

Engineering a Synthetic Formatotrophic Yeast With Enhanced Growth Capacity on Formate

open access: yesAdvanced Energy and Sustainability Research, Volume 7, Issue 5, May 2026.
Engineering Yeast for Growth on Formate and Methanol via Serine‐Centered C1 Coupling. By engineering serine metabolism and remodeling the rGly pathway, we rendered yeast growth strictly dependent on formate. This synthetic formatotrophy enables growth on dual‐C1 substrates by channeling formate into the central metabolism via serine deaminases and ...
Shuyuan Guo   +11 more
wiley   +1 more source

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