Untargeted metabolomics analysis of Ralstonia eutropha during plant oil cultivations reveals the presence of a fucose salvage pathway [PDF]
Scientific Reports, 2021 Process engineering of biotechnological productions can benefit greatly from comprehensive analysis of microbial physiology and metabolism. Ralstonia eutropha (syn.
Björn Gutschmann +5 more
doaj +2 more sources
A novel gene expression system for Ralstonia eutropha based on the T7 promoter [PDF]
BMC Microbiology, 2020 Background Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages.
Muzi Hu +7 more
doaj +2 more sources
Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 [PDF]
BioImpacts, 2021 Introduction: Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics.
Farzaneh Barati +3 more
doaj +2 more sources
Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique [PDF]
Biotechnology for Biofuels, 2018 Background Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO2 fixation, which makes it a potential strain for industrial PHA production and attractive host for CO2 conversion.
Bin Xiong +5 more
doaj +2 more sources
Engineering the Calvin–Benson–Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production [PDF]
Microbial Cell Factories, 2020 Background CO2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin–Benson–Bassham (CBB) cycle is the most important and widespread carbon fixation pathway.
Zhongkang Li +5 more
doaj +2 more sources
Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds [PDF]
Microbial Cell Factories, 2019 Background Poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial polyester with high biodegradability, even in marine environments.
Mengxiao Zhang +4 more
doaj +2 more sources
Production of fatty acids in Ralstonia eutropha H16 by engineering β-oxidation and carbon storage [PDF]
PeerJ, 2015 Ralstonia eutropha H16 is a facultatively autotrophic hydrogen-oxidizing bacterium capable of producing polyhydroxybutyrate (PHB)-based bioplastics. As PHB’s physical properties may be improved by incorporation of medium-chain-length fatty acids (MCFAs),
Janice S. Chen +5 more
doaj +3 more sources
Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity [PDF]
Microbial Cell Factories, 2019 Background The chemolithoautotrophic β-proteobacterium Ralstonia eutropha H16 (Cupriavidus necator) is one of the most studied model organisms for growth on H2 and CO2. R.
Anna Poladyan +4 more
doaj +2 more sources
Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining [PDF]
Bio-Protocol, 2018 Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas ...
Janina Juengert +2 more
doaj +2 more sources
A Comprehensive Safety Assessment of Ralstonia eutropha H16 for Food Applications: Integrating Genomic, Phenotypic, and Toxicological Analyzes [PDF]
MicroorganismsRalstonia eutropha H16, a metabolically versatile bacterium, has gained prominence as a microbial platform for sustainable bioproduction. While its capabilities in synthesizing single-cell proteins and biodegradable materials are well documented ...
Xiaoyan You +8 more
doaj +2 more sources