Results 1 to 10 of about 16,736 (223)

Untargeted metabolomics analysis of Ralstonia eutropha during plant oil cultivations reveals the presence of a fucose salvage pathway [PDF]

Scientific Reports, 2021
Process engineering of biotechnological productions can benefit greatly from comprehensive analysis of microbial physiology and metabolism. Ralstonia eutropha (syn.
Björn Gutschmann, Martina C. E. Bock, Stefan Jahns, Peter Neubauer, Christopher J. Brigham, Sebastian L. Riedel   +5 more
doaj   +3 more sources

Engineering the Calvin–Benson–Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production [PDF]

Microbial Cell Factories, 2020
Background CO2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin–Benson–Bassham (CBB) cycle is the most important and widespread carbon fixation pathway.
Zhongkang Li, Xiuqing Xin, Bin Xiong, Dongdong Zhao, Xueli Zhang, Changhao Bi   +5 more
doaj   +3 more sources

Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 [PDF]

BioImpacts, 2021
Introduction: Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics.
Farzaneh Barati, Ezat Asgarani, Sara Gharavi, Mohammad Reza Soudi   +3 more
doaj   +3 more sources

A novel gene expression system for Ralstonia eutropha based on the T7 promoter [PDF]

BMC Microbiology, 2020
Background Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages.
Muzi Hu, Bin Xiong, Zhongkang Li, Li Liu, Siwei Li, Chunzhi Zhang, Xueli Zhang, Changhao Bi   +7 more
doaj   +3 more sources

Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique [PDF]

Biotechnology for Biofuels, 2018
Background Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO2 fixation, which makes it a potential strain for industrial PHA production and attractive host for CO2 conversion.
Bin Xiong, Zhongkang Li, Li Liu, Dongdong Zhao, Xueli Zhang, Changhao Bi   +5 more
doaj   +3 more sources

Polyhydroxyalkanoate Copolymer Production by Recombinant Ralstonia eutropha Strain 1F2 from Fructose or Carbon Dioxide as Sole Carbon Source [PDF]

Bioengineering
Ralstonia eutropha strain H16 is a chemoautotrophic bacterium that oxidizes hydrogen and accumulates poly[(R)-3-hydroxybutyrate] [P(3HB)], a prominent polyhydroxyalkanoate (PHA), within its cell. R.
Chih-Ting Wang, Ramamoorthi M Sivashankari, Yuki Miyahara, Takeharu Tsuge   +3 more
doaj   +3 more sources

Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds [PDF]

Microbial Cell Factories, 2019
Background Poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial polyester with high biodegradability, even in marine environments.
Mengxiao Zhang, Shunsuke Kurita, Izumi Orita, Satoshi Nakamura, Toshiaki Fukui   +4 more
doaj   +3 more sources

Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining [PDF]

Bio-Protocol, 2018
Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas ...
Janina Juengert, Stephanie Bresan, Dieter Jendrossek   +2 more
doaj   +3 more sources

Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity [PDF]

Microbial Cell Factories, 2019
Background The chemolithoautotrophic β-proteobacterium Ralstonia eutropha H16 (Cupriavidus necator) is one of the most studied model organisms for growth on H2 and CO2. R.
Anna Poladyan, Syuzanna Blbulyan, Mayramik Sahakyan, Oliver Lenz, Armen Trchounian   +4 more
doaj   +3 more sources

(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha. [PDF]

Appl Microbiol Biotechnol, 2023
Bacterial polyhydroxyalkanoates (PHAs) are promising bio-based biodegradable polyesters. It was recently reported that novel PHA block copolymers composed of (R)-3-hydroxybutyrate (3HB) and (R)-2-hydroxybutyrate (2HB) were synthesized by Escherichia coli
Ishihara S, Orita I, Matsumoto K, Fukui T.   +3 more
europepmc   +2 more sources