Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator). [PDF]
Synthetic biology encompasses many kinds of ideas and techniques with the common theme of creating something novel. The industrially relevant microorganism, Ralstonia eutropha (also known as Cupriavidus necator), has long been a subject of metabolic ...
Santolin L, Riedel SL, Brigham CJ.
europepmc +4 more sources
Expression of the entire polyhydroxybutyrate operon of Ralstonia eutropha in plants [PDF]
Background Previously we demonstrated that an entire bacterial operon (the PRN operon) is expressible in plants when driven by the Tomato -yellow-leaf-curl-virus (TYLCV) -derived universal vector IL-60.
Rita Mozes-Koch +6 more
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Production of fatty acids in Ralstonia eutropha H16 by engineering β-oxidation and carbon storage [PDF]
Ralstonia eutropha H16 is a facultatively autotrophic hydrogen-oxidizing bacterium capable of producing polyhydroxybutyrate (PHB)-based bioplastics. As PHB’s physical properties may be improved by incorporation of medium-chain-length fatty acids (MCFAs),
Janice S. Chen +5 more
doaj +4 more sources
L-amino acids affect the hydrogenase activity and growth of Ralstonia eutropha H16. [PDF]
Ralstonia eutropha H16 is a chemolithoautotrophic bacterium with O_2-tolerant hydrogenase (Hyds) enzymes. Hyds are expressed in the presence of gas mixtures (H_2, O_2, CO_2) or under energy limitation and stress conditions.
Iskandaryan M +5 more
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Prospects for engineering Ralstonia eutropha and Zymomonas mobilis for the autotrophic production of 2,3-butanediol from CO2 and H2. [PDF]
The decarbonization of the chemical industry and a shift toward circular economies because of high global CO2 emissions make CO2 an attractive feedstock for manufacturing chemicals.
Wei H +6 more
europepmc +2 more sources
Tung Oil-Based Production of High 3-Hydroxyhexanoate-Containing Terpolymer Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate-co-3-Hydroxyhexanoate) Using Engineered Ralstonia eutropha. [PDF]
Polyhydroxyalkanoates (PHAs) are attractive new bioplastics for the replacement of plastics derived from fossil fuels. With their biodegradable properties, they have also recently been applied to the medical field.
Lee HS +11 more
europepmc +2 more sources
Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli. [PDF]
Hydrogenases are abundant metalloenzymes that catalyze the reversible conversion of molecular H2 into protons and electrons. Important achievements have been made over the past two decades in the understanding of these highly complex enzymes.
Fan Q +5 more
europepmc +2 more sources
Elucidating regulation of polyhydroxyalkanoate metabolism in Ralstonia eutropha: Identification of transcriptional regulators from phasin and depolymerase genes. [PDF]
Despite the ever-growing research interest in polyhydroxyalkanoates (PHAs) as green plastic alternatives, our understanding of the regulatory mechanisms governing PHA synthesis, storage, and degradation in the model organism Ralstonia eutropha remains ...
Santolin L +11 more
europepmc +2 more sources
The Multiple Roles of Polyphosphate in Ralstonia eutropha and Other Bacteria
An astonishing variety of functions has been attributed to polyphosphate (polyP) in prokaryotes. Besides being a reservoir of phosphorus, functions in exopolysaccharide formation, motility, virulence and in surviving various forms of stresses such as ...
H. Rosigkeit +3 more
semanticscholar +6 more sources
A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate. [PDF]
Polyphosphosphate kinases (PPKs) catalyse the reversible transfer of the γ-phosphate group of a nucleoside-triphosphate to a growing chain of polyphosphate. Most known PPKs are specific for ATP, but some can also use GTP as a phosphate donor.
Hildenbrand JC, Teleki A, Jendrossek D.
europepmc +2 more sources

