Results 221 to 230 of about 10,252,281 (255)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

<i>V</i><sub>TH</sub>-Adjustable p-Channel GaN Field-Effect Transistor with an Inserted n-GaN Layer. [PDF]

open access: yesMicromachines (Basel)
Liu Y   +10 more
europepmc   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

open access: yesFEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia   +9 more
wiley   +1 more source

Leveraging prompt-driven generative AI for systematic reviews in digital psychiatry: A stage-matched comparative proof-of-concept for healthcare researchers and clinicians. [PDF]

open access: yesPLOS Digit Health
Parkington K   +19 more
europepmc   +1 more source

Dual-function floating-gate memory driver for energy-efficient integrated display-illumination system. [PDF]

open access: yesNat Commun
Wu H   +20 more
europepmc   +1 more source

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