We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Harnessing in-situ tribo-plasma emission for energy harvesting. [PDF]
Zeng Q +5 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
<i>V</i><sub>TH</sub>-Adjustable p-Channel GaN Field-Effect Transistor with an Inserted n-GaN Layer. [PDF]
Liu Y +10 more
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Polarization-Multiplexed Chaotic LiDAR Based on a VCSEL with Delayed Orthogonal Feedback. [PDF]
Wang T +7 more
europepmc +1 more source
Gate Metal-Driven Sensing and Memory Bifunctionality in Intrinsically Stretchable Organic Electrochemical Transistors for Soft Neuromorphic Systems. [PDF]
Yoon J +8 more
europepmc +1 more source
Design of a single inductor bidirectional DC converter for V2V energy transfer: On board charger architecture. [PDF]
Mostafa HH +3 more
europepmc +1 more source
Leveraging prompt-driven generative AI for systematic reviews in digital psychiatry: A stage-matched comparative proof-of-concept for healthcare researchers and clinicians. [PDF]
Parkington K +19 more
europepmc +1 more source
Dual-function floating-gate memory driver for energy-efficient integrated display-illumination system. [PDF]
Wu H +20 more
europepmc +1 more source

