Results 191 to 200 of about 1,081,533 (280)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

On physical analysis of phthalocyanine iron (II) using topological descriptor and curve fitting models. [PDF]

Sci Rep
Hanif MF, Mahmood H, Ahmad S, Fiidow MA.
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

On analysis of topological indices and heat of formation for benzyl sulfamoyl network via curve fitting model. [PDF]

Sci Rep
Yang H, Hanif MF, Siddiqui MK, Hanif MF, Maqbool A, Fiidow MA.   +5 more
europepmc   +1 more source

A new framework for growth curve fitting based on the von Bertalanffy Growth Function. [PDF]

Sci Rep, 2020
Lee L, Atkinson D, Hirst AG, Cornell SJ.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Author Correction: On physical analysis of phthalocyanine iron (II) using topological descriptor and curve fitting models. [PDF]

Sci Rep
Hanif MF, Mahmood H, Ahmad S, Fiidow MA.
europepmc   +1 more source

Reproducibility of liver iron concentration estimates in MRI through R2* measurement determined by least-squares curve fitting. [PDF]

J Appl Clin Med Phys, 2020
Headley AM, Grice JV, Pickens DR.
europepmc   +1 more source

Curve Fitting [PDF]

Nature, 1936
openaire   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source