Results 151 to 160 of about 130,249 (264)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments. [PDF]

Tree Physiol
Miranda MT, Pereira L, Pires GS, Guan X, Silva LM, Kreinert S, Machado EC, Jansen S, Ribeiro RV.   +8 more
europepmc   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

CitPH4 Confers Resistance to Citrus Canker by Activating Papain-Like Cysteine Protease. [PDF]

Plant Biotechnol J
Yuan T, Huang R, Lu Z, Zhao Y, Lv J, He J, Lin S, He C, Liu X, Yang K, Fu J, Sun J, Liao L, Zheng W, Zhang W, Jia H, Xu Y, Wang X, Xu Q, Ding F, Hu B.   +20 more
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Silver nanoparticles derived from Calotropis and Plumeria plants as a green approach to extend the vase life of Alstroemeria flowers. [PDF]

Sci Rep
Abid N, Samsampour D, Babarabie M.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Complete genome sequence of <i>Xanthomonas campestris</i> pv. <i>campestris</i> strain NSTU_AG-1 causing black rot disease isolated from infected cabbage leaves in Bangladesh. [PDF]

Microbiol Resour Announc
Goswami A, Nayem MR, Jhan PK, Khatun A, Rahman AN, Hasan M, Dutta S, Islam MT, Park J-I, Rubel MH.   +9 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Evaluation of Photoprotective Strategies in Asexual <i>Michelia guangdongensis</i> Lines Under High Temperature and Strong Light Stress Using the Entropy-Weighted TOPSIS Method. [PDF]

Plants (Basel)
Liu J, Xu F, Chen X, Wang Y, Deng Z, Bai Q, Liao H, Zhu B, Zhang W.   +8 more
europepmc   +1 more source