Results 131 to 140 of about 780,101 (308)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Cutting Test Device for PDC Bit with Mixed Cutter Arrangement

open access: yesShiyou jixie, 2019
The existing PDC bit single-cutter test device is very inconvenient to adjust the parameters such as single-cutter rake angle. It is necessary to carry out frequent replacement of multiple sets of tools, which is not suitable for the mixed cutting ...
Liu Zhong   +3 more
doaj  

An Optimization Framework to Improve 4D-Var Data Assimilation System Performance

open access: yes, 2013
This paper develops a computational framework for optimizing the parameters of data assimilation systems in order to improve their performance. The approach formulates a continuous meta-optimization problem for parameters; the meta-optimization is ...
Cioaca, Alexandru, Sandu, Adrian
core   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

open access: yesFEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde   +3 more
wiley   +1 more source

Skúšanie opakovanej presnosti polohovania plazmovej rezacej hlavy [PDF]

open access: yes, 2005
Článok stručne opisuje experimentov zameraných na overenie vybraných technologických parametrov plazmového rezacieho stroja. Konštrukčné riešenie tohto stroja predstavuje komplexnú kinematickú štruktúru s deviatimi stupňami voľnosti.
Gros, Peter   +2 more
core   +1 more source

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