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Performance of immobilized recombinant Escherichia coli on β‐cyclodextrin glucanotransferase secretion and cell viability in a membrane bioreactor

Journal of Chemical Technology & Biotechnology
The efficiency of recombinant enzyme secretion into the medium is essential for simplifying purification and increasing yield. However, Escherichia coli, a commonly used expression host, faces challenges such as cell lysis and plasmid instability during ...
N. N. H. Baharudin   +5 more
semanticscholar   +1 more source

Preparation of Cyclodextrin Glucanotransferase‐Modified Starch for Enhanced Freeze‐Thaw Stability of Dumpling Wrappers

Starke (Weinheim)
Amylopectin cluster (APC), a modified starch prepared using cyclodextrin glucanotransferase (CGTase), was utilized as a freeze–thaw stabilizer to improve the quality of dumpling wrappers containing Baromi 2, a rice variety used as a substitute for wheat ...
Jin‐Woong Baek   +8 more
semanticscholar   +1 more source

Improvement of combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase by functionalization of cross-linker for maltooligosaccharides synthesis.

International Journal of Biological Macromolecules
Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase (CGTase) and maltogenic amylase (Mag1) from Bacillus lehensis G1 (Combi-CLEAs-CM) were successfully developed to synthesis maltooligosaccharides (MOS). Yet, the poor cross-linking
Yee Seng Yip   +5 more
semanticscholar   +1 more source

A review on recent upgradation and strategies to enhance cyclodextrin glucanotransferase properties for its applications.

International Journal of Biological Macromolecules
Cyclodextrin glycosyltransferase (CGTase) is a significant extracellular enzyme with diverse functions. CGTase is widely used in production of cyclic α-(1,4)-linked oligosaccharides (cyclodextrins) from starch via transglycosylation reaction.
Khushbu Rabadiya   +5 more
semanticscholar   +1 more source

Genetic and biochemical characterization of thermophilic β-cyclodextrin glucanotransferase from Gracilibacillus alcaliphilus SK51.001.

The Journal of the Science of Food and Agriculture, 2020
BACKGROUND A strain, Gracilibacillus alcaliphilus SK51.001, which produces β-CGTase (β-cyclodextrin glucanotransferase) (EC 2.4.1.19), was screened and isolated from Sudanese soil. The objective of this study was to sequence and characterize the β-CGTase
Mohammed Abdalla   +5 more
semanticscholar   +1 more source

From random to chemoselective cross-linking: Reorientation and enhancement of immobilized cross-linked enzyme aggregates of cyclodextrin glucanotransferase from Shouchella lehensis G1 via targeted domain modification.

International Journal of Biological Macromolecules
Uncontrolled, random cross-linking near the enzyme's active site may impede mass transfer and overall activity, which is one of the challenges in cross-linked enzyme aggregates (CLEAs) immobilization.
N. Jailani   +6 more
semanticscholar   +1 more source

Cyclodextrin glucanotransferase: fundamentals and biotechnological implications

Applied Microbiology and Biotechnology, 2023
Dimple S. Pardhi   +5 more
semanticscholar   +1 more source

Efficient production of α-monoglucosyl hesperidin by cyclodextrin glucanotransferase from Bacillus subtilis

Applied Microbiology and Biotechnology, 2023
Jiawei Zhou   +6 more
semanticscholar   +1 more source

Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration

Journal of Chemical Technology & Biotechnology, 2023
R. C. Man   +5 more
semanticscholar   +1 more source

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