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Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production [PDF]
Biotechnology Letters, 2011 Two multivariate statistical methods, factor analysis (FA) and hierarchical cluster analysis (HCA), were applied to experimental data set to evaluate their usefulness in selecting the adequate expression system and optimal growth parameters for recombinant cyprosin B production. Using FA, the large data set was reduced to two factors representing 73.4%
Luís Fonseca +2 more
exaly +5 more sources
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Bioprocess and Biosystems Engineering, 2014
Nowadays, the dairy industry is continuously looking for new and more efficient clotting enzymes to create innovative products. Cyprosin B is a plant aspartic protease characterized by clotting activity that was previously cloned in Saccharomyces cerevisiae BJ1991 strain.
Luís Fonseca +2 more
exaly +3 more sources
Nowadays, the dairy industry is continuously looking for new and more efficient clotting enzymes to create innovative products. Cyprosin B is a plant aspartic protease characterized by clotting activity that was previously cloned in Saccharomyces cerevisiae BJ1991 strain.
Luís Fonseca +2 more
exaly +3 more sources
Plant Cell, Tissue and Organ Culture, 2010
Cynara cardunculus suspension cells were transformed by particle bombardment to overexpress the cypro11 gene coding for cyprosin B. Green fluorescent protein, used as a visual reporter through mgfp4-ER gene, facilitates the screening of transformed cells at the initial stages when antibiotics cause generalized cell death.
Susana Serrazina
exaly +2 more sources
Cynara cardunculus suspension cells were transformed by particle bombardment to overexpress the cypro11 gene coding for cyprosin B. Green fluorescent protein, used as a visual reporter through mgfp4-ER gene, facilitates the screening of transformed cells at the initial stages when antibiotics cause generalized cell death.
Susana Serrazina
exaly +2 more sources
Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. [PDF]
FEBS Journal, 2007 Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese.
Carlos Faro +2 more
exaly +2 more sources
Journal of Bioscience and Bioengineering, 2008
The Saccharomyces cerevisiae W303-1A strain transformed with a centromeric plasmid containing CYPRO11, which codifies the aspartic protease cyprosin B, was grown in a 3 l bioreactor under aerobic conditions. Expression of cyprosin B is directly dependent on the concentration of galactose used as the inducer and carbon source in 1% yeast extract, 2 ...
Pedro N, Sampaio +4 more
openaire +2 more sources
The Saccharomyces cerevisiae W303-1A strain transformed with a centromeric plasmid containing CYPRO11, which codifies the aspartic protease cyprosin B, was grown in a 3 l bioreactor under aerobic conditions. Expression of cyprosin B is directly dependent on the concentration of galactose used as the inducer and carbon source in 1% yeast extract, 2 ...
Pedro N, Sampaio +4 more
openaire +2 more sources
Production and characterization of recombinant cyprosin B in (W303-1A) strain
Journal of Bioscience and Bioengineering, 2008Ana Margarida Fortes +2 more
exaly
near infrared spectroscopy monitoring of cyprosin production by recombinant strains
Journal of Biotechnology, 2014Marta B. Lopes +2 more
exaly
Use of Recombinant Cyprosin in the Manufacture of Ewe's Milk Cheese
Journal of Agricultural and Food Chemistry, 2003Luis Tejada +2 more
exaly