Results 181 to 190 of about 9,325 (207)

CyTOF: An Emerging Technology for Single-Cell Proteomics in the Mouse

Current Protocols, 2021
AbstractThe ability to analyze the proteome of single cells is critical for the advancement of studies of steady‐state and pathological processes. Mass cytometry, or CyTOF, combines principles of mass spectrometry and flow cytometry to enable single‐cell analysis of protein expression.
Monica J Justice, Yeji An, Lauren J Tracey   +2 more
exaly   +3 more sources

Immunometabolism the CyTOF way

Immunity, 2021
In this issue of Immunity, Levine et al. report a CyTOF-based approach for the analyses of CD8+ T cells metabolic changes at the single-cell level. This approach identified a transition state early in T cell activation that is characterized by high glycolytic and oxidative activity, providing new insight into the metabolic changes that underlie the ...
Liujia, Qian, Tiannan, Guo
openaire   +2 more sources

A CD45-based barcoding approach to multiplex mass-cytometry (CyTOF) [PDF]

Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2015
AbstractCyTOF enables the study of the immune system with a complexity, depth, and multidimensionality never achieved before. However, the full potential of using CyTOF can be limited by scarce cell samples. Barcoding strategies developed based on direct labeling of cells using maleimido‐monoamide‐DOTA (m‐DOTA) provide a very useful tool.
Salvatore Albani, Juntao Li, Liyun Lai
exaly   +3 more sources

CyTOF Mass Cytometry for Click Proliferation Assays

Current Protocols in Cytometry, 2017
AbstractNovel cell analyzers, including polychromatic flow cytometers and isotopical cytometry by time of flight (CyTOF) mass cytometers, enable simultaneous measurement of virtually bondless characteristics at the single‐cell level. BrdU assays for quantifying cellular proliferation are common but have several limitations, including the need for a DNA
Egor Ulashchik, Paolo Cappella, Vinko Tosevski   +2 more
exaly   +4 more sources

CIMAC-CIDC CyTOF harmonization.

Journal of Clinical Oncology, 2020
e15242 Background: The Cancer Immune Monitoring and Analysis Centers – Cancer Immunology Data Commons (CIMAC-CIDC) network is a National Cancer Institute-funded initiative to identify biomarkers of mechanisms and response to cancer immunotherapy clinical trials, using state-of-the-art assay technologies.
Adeeb Rahman, Bita Sahaf, Melanie Davila, Nicholas Fernandez, Emily McWilliams, Karen Millerchip, Salah E Bentebibel, Cara L. Haymaker, Natalia Sigal, Caroline Duault, Emily Thrash, Diane Del Valle, Beatriz Sanchez Espiridion, Mina Pichavant, Chantale Bernatchez, Ignacio Ivan Wistuba, Sacha Gnjatic, Sean Bendall, Holden Maecker   +18 more
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CyTOF®: A New Tool to Decipher the Immunomodulatory Activity of Daratumumab [PDF]

Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2019
Barbara Castella, Roberto Mina, Francesca Gay   +2 more
exaly   +4 more sources

Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

2022
Regulation of hematopoiesis is dependent upon interactions between hematopoietic stem/progenitor cells and niche components, requiring a highly diverse array of different cell-cell interactions and cell signaling events. The overwhelming diversity of the components that can regulate hematopoiesis, especially when factoring in how the cell surface and ...
Safa F, Mohamad, Maegan L, Capitano
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Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)

Methods in Molecular Biology, 2022
Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological ...
Daniel H Gray, Charis E Teh, Tania Tan
exaly   +3 more sources

CyTOF Measurement of Immunocompetence Across Major Immune Cell Types [PDF]

Current Protocols in Cytometry, 2017
AbstractThe central role of the immune system is becoming appreciated in a wide variety of diseases. Cancer immunotherapy is one area that has yielded much recent success, although not all patients benefit equally. At the same time, recent studies have highlighted the heterogeneity of the human immune system.
Priyanka Balasubrahmanyam, Holden T. Maecker   +1 more
exaly   +3 more sources

Graph Partitioning Algorithms and Applications on CyTOF Experiments

2023
Graph partitioning is a fundamental problem in computer science and engineering with diverse applications in fields such as circuit design, network analysis, and data mining [6]. The goal of graph partitioning is to divide a given graph into multiple clusters, with the aim of minimizing certain criteria, such as the number of edges between clusters ...
openaire   +1 more source