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Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2017 AbstractImaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy ...
Iram Siddiqui
exaly +3 more sources
An Improved Method for Extracting, Sorting, and AMS Dating of Pollen Concentrates From Lake Sediment
Frontiers in Ecology and Evolution, 2021 High-resolution chronologies are crucial for paleoenvironmental reconstructions, and are particularly challenging for lacustrine records of terrestrial paleoclimate.
Irene Tunno +3 more
doaj +1 more source
Single-cell phenotypes of peripheral blood immune cells in early and late stages of non-alcoholic fatty liver disease [PDF]
Clinical and Molecular Hepatology, 2023 Background/Aims Immune and inflammatory cells respond to multiple pathological hits in the development of nonalcoholic steatohepatitis (NASH) and fibrosis.
Kathryn Jane Waller +7 more
doaj +1 more source
CyTOF protocol for immune monitoring of solid tumors from mouse models
STAR Protocols, 2023 Summary: Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter ...
Sarah M. Shin +8 more
doaj +1 more source
High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
STAR Protocols, 2022 Summary: This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry.
Amira Ben Amara +12 more
doaj +1 more source
Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
STAR Protocols, 2021 Summary: T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer.
Ioannis Skordos +2 more
doaj +1 more source
Mass cytometry immunostaining protocol for multiplexing clinical samples
STAR Protocols, 2022 Summary: This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination ...
Ramy Gadalla +3 more
doaj +1 more source
STAR Protocols, 2023 Summary: Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4+ T cell differentiation in the intestine.
Jinzhi Duan +3 more
doaj +1 more source
STAR Protocols, 2022 Summary: Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune
Calvin Krollmann +6 more
doaj +1 more source
Flow Cytometry II: Mass and Imaging Cytometry [PDF]
Journal of Investigative Dermatology, 2015 Correction to: Journal of Investigative Dermatology (2015) 135, e36; doi:10.1038/id.2015 ...
Doan, Hung +2 more
openaire +3 more sources