Results 1 to 10 of about 351,921 (163)
Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2017 AbstractImaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy ...
Qing, Chang +5 more
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Mass Cytometry Imaging for the Study of Human Diseases—Applications and Data Analysis Strategies
Frontiers in Immunology, 2019 High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to ...
Heeva Baharlou +9 more
doaj +3 more sources
Minimizing Batch Effects in Mass Cytometry Data
Frontiers in Immunology, 2019 Cytometry by Time-Of-Flight (CyTOF) uses antibodies conjugated to isotopically pure metals to identify and quantify a large number of cellular features with single-cell resolution.
Ronald P. Schuyler +9 more
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Evaluation of Mass Cytometry in the Clinical Laboratory [PDF]
Cytometry Part B - Clinical Cytometry, 2019 BackgroundMass cytometry can differentiate more channels than conventional flow cytometry. However, for clinical use, standardization and agreement with well‐established methods is paramount. We compared mass cytometry to standard clinical flow cytometry.MethodsMass and flow cytometry were performed in parallel on peripheral blood samples from 25 ...
Eugene V, Ravkov +12 more
exaly +3 more sources
Single-cell phenotypes of peripheral blood immune cells in early and late stages of non-alcoholic fatty liver disease [PDF]
Clinical and Molecular Hepatology, 2023 Background/Aims Immune and inflammatory cells respond to multiple pathological hits in the development of nonalcoholic steatohepatitis (NASH) and fibrosis.
Kathryn Jane Waller +7 more
doaj +1 more source
An Improved Method for Extracting, Sorting, and AMS Dating of Pollen Concentrates From Lake Sediment
Frontiers in Ecology and Evolution, 2021 High-resolution chronologies are crucial for paleoenvironmental reconstructions, and are particularly challenging for lacustrine records of terrestrial paleoclimate.
Irene Tunno +3 more
doaj +1 more source
CyTOF protocol for immune monitoring of solid tumors from mouse models
STAR Protocols, 2023 Summary: Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter ...
Sarah M. Shin +8 more
doaj +1 more source
High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
STAR Protocols, 2022 Summary: This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry.
Amira Ben Amara +12 more
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Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
STAR Protocols, 2021 Summary: T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer.
Ioannis Skordos +2 more
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Mass cytometry immunostaining protocol for multiplexing clinical samples
STAR Protocols, 2022 Summary: This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination ...
Ramy Gadalla +3 more
doaj +1 more source