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Polymer-Based Mass Cytometry Reagents: Synthesis and Biomedical Applications [PDF]
MoleculesMass cytometry has promoted the development of single-cell analysis by enabling the highly multiplexed detection of cellular markers using metal-tagged antibodies or cells.
Yin-Feng Wang, Wenying Wu, Ya-Hui Ge
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spillR: spillover compensation in mass cytometry data. [PDF]
Bioinformatics, 2023 Abstract Motivation Channel interference in mass cytometry can cause spillover and may result in miscounting of protein markers. Chevrier et al. introduce an experimental and computational procedure to estimate and compensate for spillover implemented in their R package CATALYST.
Guazzini M +3 more
europepmc +6 more sources
Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2017 AbstractImaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy ...
Olga I Ornatsky +2 more
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Mass Cytometry Imaging for the Study of Human Diseases—Applications and Data Analysis Strategies
Frontiers in Immunology, 2019 High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to ...
Heeva Baharlou +9 more
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Single-cell mass cytometry in immunological skin diseases [PDF]
Frontiers in ImmunologyImmune-related skin diseases represent a collective of dermatological disorders intricately linked to dysfunctional immune system processes. These conditions are primarily characterized by an immoderate activation of the immune system or deviant immune ...
Mingming Zhao +15 more
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An Improved Method for Extracting, Sorting, and AMS Dating of Pollen Concentrates From Lake Sediment
Frontiers in Ecology and Evolution, 2021 High-resolution chronologies are crucial for paleoenvironmental reconstructions, and are particularly challenging for lacustrine records of terrestrial paleoclimate.
Irene Tunno +3 more
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CyTOF protocol for immune monitoring of solid tumors from mouse models
STAR Protocols, 2023 Summary: Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter ...
Sarah M. Shin +8 more
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High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
STAR Protocols, 2022 Summary: This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry.
Amira Ben Amara +12 more
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Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
STAR Protocols, 2021 Summary: T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer.
Ioannis Skordos +2 more
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Mass cytometry immunostaining protocol for multiplexing clinical samples
STAR Protocols, 2022 Summary: This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination ...
Ramy Gadalla +3 more
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