Results 31 to 40 of about 359,525 (166)
FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
STAR Protocols, 2023 Summary: Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Camilla Tiezzi +12 more
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Stabilizing Antibody Cocktails for Mass Cytometry [PDF]
Cytometry Part A, 2019 AbstractMass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective data by computational analyses, several techniques have been developed to minimize noise in mass cytometric data sets, such as sample banking ...
Schulz, Axel R. +5 more
openaire +2 more sources
Minimizing Batch Effects in Mass Cytometry Data
Frontiers in Immunology, 2019 Cytometry by Time-Of-Flight (CyTOF) uses antibodies conjugated to isotopically pure metals to identify and quantify a large number of cellular features with single-cell resolution.
Ronald P. Schuyler +9 more
doaj +1 more source
FACS-assisted CRISPR-Cas9 genome editing of human induced pluripotent stem cells
STAR Protocols, 2022 Summary: This manuscript proposes an efficient and reproducible protocol for the generation of genetically modified human induced pluripotent stem cells (hiPSCs) by genome editing using CRISPR-Cas9 technology.
Amandine Caillaud +15 more
doaj +1 more source
STAR Protocols, 2021 Summary: Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ’s immunity and function and are transferred during transplantation.
Amy Prosser +3 more
doaj +1 more source
STAR Protocols, 2022 Summary: The Golgi apparatus is subjected to fragmentation under several cellular processes such as mitosis. Here we describe two complementary approaches to analyze different Golgi morphological changes during its mitotic fragmentation, using classical ...
Inbal Wortzel +3 more
doaj +1 more source
MASS CYTOMETRY REVEALS A DISTINCT IMMUNOPHENOTYPE IN TELOMEROPATHIES
Hematology, Transfusion and Cell Therapy, 2023 Telomere-biology disorders (TBDs) are characterized by short telomeres and defective telomere repair, and clinical manifestations include bone marrow failure (BMF), myeloid neoplasms, fibrotic lung disease and liver cirrhosis.
W Gomes +7 more
doaj +1 more source
Platelet surface marker analysis by mass cytometry
Platelets, 2020 Mass cytometry is a next generation flow cytometry technology which analyzes cells one at a time (up to 1000/sec) using mass spectrometry to detect probes labeled with rare-earth metals.
Thomas A. Blair, Andrew L. Frelinger
doaj +1 more source
Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry
Genomics, Proteomics & Bioinformatics, 2021 Single-cell mass cytometry (SCMC) combines features of traditional flow cytometry (i.e., fluorescence-activated cell sorting) with mass spectrometry, making it possible to measure several parameters at the single-cell level for a complex analysis of ...
József Á. Balog +6 more
doaj +1 more source
Evaluation of Mass Cytometry in the Clinical Laboratory [PDF]
Cytometry Part B: Clinical Cytometry, 2019 BackgroundMass cytometry can differentiate more channels than conventional flow cytometry. However, for clinical use, standardization and agreement with well‐established methods is paramount. We compared mass cytometry to standard clinical flow cytometry.MethodsMass and flow cytometry were performed in parallel on peripheral blood samples from 25 ...
Eugene V, Ravkov +12 more
openaire +2 more sources