Results 31 to 40 of about 348,965 (301)

Stabilizing Antibody Cocktails for Mass Cytometry [PDF]

Cytometry Part A, 2019
AbstractMass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective data by computational analyses, several techniques have been developed to minimize noise in mass cytometric data sets, such as sample banking ...
Schulz, Axel R., Baumgart, Sabine, Schulze, Julia, Urbicht, Marie, Grützkau, Andreas, Mei, Henrik E.   +5 more
openaire   +2 more sources

Enabling Indium Channels for Mass Cytometry by Using Reinforced Cyclambased Chelating Polylysine

, 2020
A metal containing polymer (MCP) based on a polylysine functionalized by In(III) chelates was synthesized. The chelator is based on a constrained dipicolinate cyclam that forms a highly inert In(III) complex.
Raphael, Tripier, Maryline, Beyler, Peng, Liu, Nick, Zabinyakov, olga, ornatsky, Taunia L. L., Closson, Laura, Grenier, Jothir Mayanantham, Pichaandi, Mitchell A., Winnik, Yefeng, Zhang, Alexandre, Bouzekri, Vladimir, Baranov   +11 more
core   +1 more source

High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline

STAR Protocols, 2023
Summary: The current abundance of immunotherapy clinical trials presents an opportunity to learn about the underlying mechanisms and pharmacodynamic effects of novel drugs on the human immune system.
Guanning Wang, Olga Lyudovyk, Justin Y. Kim, Ya-Hui Lin, Yuval Elhanati, Divij Mathew, E. John Wherry, Ramin S. Herati, Allison R. Greenplate, Benjamin Greenbaum, Santosha A. Vardhana, Alexander C. Huang   +11 more
doaj   +1 more source

Evaluating cytokine production by flow cytometry using brefeldin A in mice

STAR Protocols, 2021
Summary: Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of ...
Stephen B. Kovacs, Changhoon Oh, Youssef Aachoui, Edward A. Miao   +3 more
doaj   +1 more source

Imaging mass cytometry data from breast cancer patient derived xenografts

, 2021
Imaging mass cytometry data from breast cancer patient derived ...
Maurizio Callari
core   +1 more source

Extended live-cell barcoding approach for multiplexed mass cytometry

Scientific Reports, 2021
Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition.
Muharrem Muftuoglu, Li Li, Shaoheng Liang, Duncan Mak, Angelique J. Lin, Junxiang Fang, Jared K. Burks, Ken Chen, Michael Andreeff   +8 more
doaj   +1 more source

Protocol for analyzing and visualizing antiviral immune responses after acute infection of the murine oral mucosa

STAR Protocols, 2021
Summary: The oral mucosa is an important site for virus infection and transmission, yet few animal models exist to examine the virology, pathology, and immunology of acute oral mucosal viral infection.
John P. Shannon, Christian R. Cherry, Sophia M. Vrba, Heather D. Hickman   +3 more
doaj   +1 more source

Characterizing Highly Cited Papers in Mass Cytometry through H-Classics [PDF]

, 2021
Mass cytometry (CyTOF) is a relatively novel technique for the multiparametric analysis of single-cell features with an increasing central role in cell biology, immunology, pharmacology, and biomedicine.
Daniel E. Di Zeo-Sánchez, Di Zeo-Sánchez, Daniel E., Camilla Stephens, Di Zeo-Sánchez, Daniel Enrique, M. Isabel Lucena, Sánchez-Núñez, Pablo, Lucena-González, María Isabel, Pablo Sánchez-Núñez, Di Zeo-Sánchez, Daniel E, Stephens, Camilla, Lucena, M Isabel, Lucena, M. Isabel   +11 more
core   +1 more source

Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence

STAR Protocols, 2022
Summary: Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling.
Xiangyu Zhang, Jeremiah Stitham, Astrid Rodriguez-Velez, Se-Jin Jeong, Arick Park, Yu-Sheng Yeh, Divya Kapoor, Bettina Mittendorfer, Babak Razani   +8 more
doaj   +1 more source

Imaging Mass Cytometry: p165

, 2020
Imaging Mass Cytometry: p165 Raw acquisitions for the paper: A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroids Vito RT Zanotelli Matthias Leutenegger Xiao‐Kang Lun Fanny Georgi ...
Vito RT Zanotelli, Bernd Bodenmiller
core   +1 more source