Results 31 to 40 of about 3,665,969 (298)

Evaluating cytokine production by flow cytometry using brefeldin A in mice

STAR Protocols, 2021
Summary: Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of ...
Stephen B. Kovacs, Changhoon Oh, Youssef Aachoui, Edward A. Miao   +3 more
doaj   +1 more source

Protocol for analyzing and visualizing antiviral immune responses after acute infection of the murine oral mucosa

STAR Protocols, 2021
Summary: The oral mucosa is an important site for virus infection and transmission, yet few animal models exist to examine the virology, pathology, and immunology of acute oral mucosal viral infection.
John P. Shannon, Christian R. Cherry, Sophia M. Vrba, Heather D. Hickman   +3 more
doaj   +1 more source

Image_5_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence

STAR Protocols, 2022
Summary: Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling.
Xiangyu Zhang, Jeremiah Stitham, Astrid Rodriguez-Velez, Se-Jin Jeong, Arick Park, Yu-Sheng Yeh, Divya Kapoor, Bettina Mittendorfer, Babak Razani   +8 more
doaj   +1 more source

Image_3_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

Extended live-cell barcoding approach for multiplexed mass cytometry

Scientific Reports, 2021
Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition.
Muharrem Muftuoglu, Li Li, Shaoheng Liang, Duncan Mak, Angelique J. Lin, Junxiang Fang, Jared K. Burks, Ken Chen, Michael Andreeff   +8 more
doaj   +1 more source

Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice

STAR Protocols, 2022
Summary: Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior ...
Michael R. D’Agostino, Sam Afkhami, Alisha Kang, Art Marzok, Matthew S. Miller, Zhou Xing   +5 more
doaj   +1 more source

Sample Preparation for Mass Cytometry Analysis [PDF]

Journal of Visualized Experiments, 2017
Mass cytometry utilizes antibodies conjugated with heavy metal labels, an approach that has greatly increased the number of parameters and opportunities for deep analysis well beyond what is possible with conventional fluorescence-based flow cytometry. As with any new technology, there are critical steps that help ensure the reliable generation of high-
Ryan L, McCarthy, Aundrietta D, Duncan, Michelle C, Barton   +2 more
openaire   +2 more sources

Image_4_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM+ cells

STAR Protocols, 2022
Summary: Lymphocytes in the mouse small intestine (SI) epithelium are critical to establish effective barrier immunity. Here, we describe a magnetic cell separation protocol that employs anti-epithelial cellular adhesion molecule (EpCAM) antibodies to ...
Praveen Prakhar, Victoria Gonzalez, Jung-Hyun Park   +2 more
doaj   +1 more source