Results 41 to 50 of about 359,525 (166)

Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

STAR Protocols, 2022
Summary: Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease.
Keltouma Benmeziane, Benoit Delache, Sébastien Langlois, Gabriella Scarlatti, Roger Le Grand, Mariangela Cavarelli   +5 more
doaj   +1 more source

Polymeric dipicolylamine based mass tags for mass cytometry

Chemical Science, 2022
A new type of metal-chelating polymer with pendant dipicolylamine chelators that bind rhenium and platinum has been developed for mass cytometry applications.
Yefeng Zhang, Peng Liu, Daniel Majonis, Mitchell A. Winnik   +3 more
openaire   +3 more sources

A combined fine needle aspiration and spectral flow cytometry approach to assess human germinal center responses to SARS-CoV-2 vaccination

STAR Protocols, 2022
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccines drive the generation of affinity-matured B cell responses through germinal center (GC) reactions in vaccine draining lymph nodes.
Emily Bettini, Katlyn Lederer, Hannah Sharpe, Mary Kaminski, Lisa Jones, Michela Locci   +5 more
doaj   +1 more source

Cell size assays for mass cytometry [PDF]

Cytometry Part A, 2016
AbstractMass cytometry offers the advantage of allowing the simultaneous measurement of a greater number parameters than conventional flow cytometry. However, to date, mass cytometry has lacked a reliable alternative to the light scatter properties that are commonly used as a cell size metric in flow cytometry (forward scatter intensity—FSC).
Alan D, Stern, Adeeb H, Rahman, Marc R, Birtwistle   +2 more
openaire   +2 more sources

Metal Isotope-Tagged Nanomaterials for Mass Cytometry and Imaging: Advances and Biomedical Applications

Nano Biomedicine and Engineering
Mass cytometry (cytometry by time-of-flight (CyTOF)) and imaging mass cytometry (IMC) are transformative technologies that combine flow cytometry principles with time-of-flight mass spectrometry (TOF-MS).
Dongling Jia, Minhui Cui, Weiwei Wang, Jielin Sun, Xiaoxiang Chen   +4 more
doaj   +1 more source

Isolation of myenteric and submucosal plexus from mouse gastrointestinal tract and subsequent flow cytometry and immunofluorescence

STAR Protocols, 2022
Summary: The myenteric plexus is located between the longitudinal and circular layers of muscularis externa in the gastrointestinal tract. It contains a large network of enteric neurons that form the enteric nervous system (ENS) and control intestinal ...
Tomasz Ahrends, Molly Weiner, Daniel Mucida   +2 more
doaj   +1 more source

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples

STAR Protocols, 2020
Summary: As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important.
Emily M. Thrash, Katja Kleinsteuber, Emma S. Hathaway, Matthew Nazzaro, Eric Haas, F. Stephen Hodi, Mariano Severgnini   +6 more
doaj   +1 more source

Protocol for identification and computational analysis of human natural killer cells using flow cytometry and R

STAR Protocols, 2023
Summary: Identifying differential protein expression is routinely used to delineate natural killer (NK) cells from various sample cohorts. This protocol describes key steps for NK cell analysis: identifying human NK cells using flow gating, data export ...
Kyle Kroll, R. Keith Reeves
doaj   +1 more source

Mass cytometry reveals immune atlas of urothelial carcinoma

BMC Cancer, 2022
Immunotherapy has emerged as a robust clinical strategy for cancer treatment. PD1/PD-L1 inhibitors have been used as second-line therapy for urothelial carcinoma due to the high tumor mutational burden.
Qing Zhang, Wenlong Zhang, Tingsheng Lin, Wenfeng Lu, Xin He, Yuanzhen Ding, Wei Chen, Wenli Diao, Meng Ding, Pingping Shen, Hongqian Guo   +10 more
doaj   +1 more source

Engineering the sialome of mammalian cells with sialic acid mimetics

STAR Protocols, 2023
Summary: Mammalian glycans show a diversity in sialic acid capping, constituting the sialome. Sialic acids can be extensively modified chemically, yielding sialic acid mimetics (SAMs).
Daniël L.A.H. Hornikx, Eline A. Visser, Venetia Psomiadou, Christian Büll, Thomas J. Boltje   +4 more
doaj   +1 more source