Results 41 to 50 of about 3,665,969 (298)

FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses

STAR Protocols, 2023
Summary: Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Camilla Tiezzi, Marzia Rossi, Andrea Vecchi, Sara Doselli, Amalia Penna, Paola Fisicaro, Ilaria Montali, Camilla Ceccatelli Berti, Valentina Reverberi, Anna Montali, Gabriele Missale, Carlo Ferrari, Carolina Boni   +12 more
doaj   +1 more source

Image_2_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

FACS-assisted CRISPR-Cas9 genome editing of human induced pluripotent stem cells

STAR Protocols, 2022
Summary: This manuscript proposes an efficient and reproducible protocol for the generation of genetically modified human induced pluripotent stem cells (hiPSCs) by genome editing using CRISPR-Cas9 technology.
Amandine Caillaud, Antoine Lévêque, Aurélie Thédrez, Aurore Girardeau, Robin Canac, Lise Bray, Manon Baudic, Julien Barc, Nathalie Gaborit, Guillaume Lamirault, Betty Gardie, Salam Idriss, Antoine Rimbert, Cédric Le May, Bertrand Cariou, Karim Si-Tayeb   +15 more
doaj   +1 more source

Stabilizing Antibody Cocktails for Mass Cytometry [PDF]

Cytometry Part A, 2019
AbstractMass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective data by computational analyses, several techniques have been developed to minimize noise in mass cytometric data sets, such as sample banking ...
Schulz, Axel R., Baumgart, Sabine, Schulze, Julia, Urbicht, Marie, Grützkau, Andreas, Mei, Henrik E.   +5 more
openaire   +2 more sources

Image_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation

STAR Protocols, 2021
Summary: Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ’s immunity and function and are transferred during transplantation.
Amy Prosser, Sarah Dart, Irma Larma-Cornwall, Michaela Lucas   +3 more
doaj   +1 more source

PeacoQC : peak-based selection of high quality cytometry data

, 2021
In cytometry analysis, a large number of markers is measured for thousands or millions of cells, resulting in high-dimensional datasets. During the measurement of these samples, erroneous events can occur such as clogs, speed changes, slow uptake of the ...
Maranon, Concepcion, Saeys, Y, Alarcon-Riquelme, Marta E., Annelies Emmaneel, Quintelier, Katrien, Maranon, C, Sichien, Dorine, Marta E. Alarcón‐Riquelme, Rybakowska, Paulina, Sofie Van Gassen, Alarcón-Riquelme, Marta E, Gert Van Isterdael, Van, Gassen S, Dorine Sichien, Van Isterdael, Gert, Van, Isterdael G, Alarcón‐Riquelme, Marta E., Concepción Marañón, Alarcon-Riquelme, ME, Emmaneel, Annelies, Emmaneel, A, Sichien, D, Yvan Saeys, Van Gassen, Sofie, Saeys, Yvan, Paulina Rybakowska, Katrien Quintelier, Quintelier, K, Rybakowska, P   +28 more
core   +1 more source

OMIP-088: Twenty-target imaging mass cytometry panel for major cell populations in mouse formalin fixed paraffin embedded liver. [PDF]

, 2023
The purpose of this 20-target imaging mass cytometry (IMC) panel is to identify the main cell types in formalin fixed paraffin embedded (FFPE) mouse liver tissue with the Hyperion™ mass cytometer from Standard BioTools (formerly Fluidigm).
Stroka, Deborah, Birrer, Fabienne, Fabienne Birrer, Tess Brodie, Deborah Stroka, Brodie, Tess   +5 more
core   +1 more source

Applying imaging flow cytometry and immunofluorescence in studying the dynamic Golgi structure in cultured cells

STAR Protocols, 2022
Summary: The Golgi apparatus is subjected to fragmentation under several cellular processes such as mitosis. Here we describe two complementary approaches to analyze different Golgi morphological changes during its mitotic fragmentation, using classical ...
Inbal Wortzel, Ziv Porat, Rony Seger, Galia Maik-Rachline   +3 more
doaj   +1 more source

Image_6_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.tif

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source