Results 41 to 50 of about 348,965 (301)
, 2023 The purpose of this 20-target imaging mass cytometry (IMC) panel is to identify the main cell types in formalin fixed paraffin embedded (FFPE) mouse liver tissue with the Hyperion™ mass cytometer from Standard BioTools (formerly Fluidigm).
Stroka, Deborah +5 more
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STAR Protocols, 2022 Summary: Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior ...
Michael R. D’Agostino +5 more
doaj +1 more source
STAR Protocols, 2022 Summary: Lymphocytes in the mouse small intestine (SI) epithelium are critical to establish effective barrier immunity. Here, we describe a magnetic cell separation protocol that employs anti-epithelial cellular adhesion molecule (EpCAM) antibodies to ...
Praveen Prakhar +2 more
doaj +1 more source
Evaluation of Mass Cytometry in the Clinical Laboratory [PDF]
Cytometry Part B: Clinical Cytometry, 2019 BackgroundMass cytometry can differentiate more channels than conventional flow cytometry. However, for clinical use, standardization and agreement with well‐established methods is paramount. We compared mass cytometry to standard clinical flow cytometry.MethodsMass and flow cytometry were performed in parallel on peripheral blood samples from 25 ...
Eugene V, Ravkov +12 more
openaire +2 more sources
, 2020 Imaging Mass Cytometry: p173 Raw acquisitions for the paper: A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroids Vito RT Zanotelli Matthias Leutenegger Xiao‐Kang Lun Fanny Georgi ...
Vito RT Zanotelli, Bernd Bodenmiller
core +1 more source
Minimizing Batch Effects in Mass Cytometry Data
Frontiers in Immunology, 2019 Cytometry by Time-Of-Flight (CyTOF) uses antibodies conjugated to isotopically pure metals to identify and quantify a large number of cellular features with single-cell resolution.
Ronald P. Schuyler +9 more
doaj +1 more source
Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry
Genomics, Proteomics & Bioinformatics, 2021 Single-cell mass cytometry (SCMC) combines features of traditional flow cytometry (i.e., fluorescence-activated cell sorting) with mass spectrometry, making it possible to measure several parameters at the single-cell level for a complex analysis of ...
József Á. Balog +6 more
doaj +1 more source
Polymeric dipicolylamine based mass tags for mass cytometry
Chemical Science, 2022 A new type of metal-chelating polymer with pendant dipicolylamine chelators that bind rhenium and platinum has been developed for mass cytometry applications.
Yefeng Zhang +3 more
openaire +3 more sources
Cell size assays for mass cytometry [PDF]
Cytometry Part A, 2016 AbstractMass cytometry offers the advantage of allowing the simultaneous measurement of a greater number parameters than conventional flow cytometry. However, to date, mass cytometry has lacked a reliable alternative to the light scatter properties that are commonly used as a cell size metric in flow cytometry (forward scatter intensity—FSC).
Alan D, Stern +2 more
openaire +2 more sources
Single Cell Detection of the p53 Protein by Mass Cytometry
, 2020 Purpose: The p53 protein and its post-translational modifications are distinctly expressed in various normal cell types and malignant cells and are usually detected by immunohistochemistry and flow cytometry in contemporary diagnostics. Here, we describe
Oda Helen Eck Fagerholt +3 more
core +1 more source