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Flow Cytometry Bioinformatics

open access: yesPLoS Computational Biology, 2013
Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational ...
Kieran O'Neill   +2 more
exaly   +5 more sources

Flow Cytometry Role in Unlocking New Frontiers for Nanomedicine Applications of Plant‐Derived Vesicles [PDF]

open access: yesJournal of Extracellular Biology
Extracellular vesicles (EVs) are nanoscale membrane‐bound vesicles released by any cell type under both physiological and pathological conditions. They carry a wide array of bioactive molecules, including microRNAs (miRNAs), lipids, proteins and other ...
Tamer Esmail   +20 more
doaj   +2 more sources

The in vitro micronucleus assay using imaging flow cytometry and deep learning

open access: yesnpj Systems Biology and Applications, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues   +6 more
doaj   +1 more source

Rapid evaluation of T cell clonality in the diagnostic work-up of mature T cell neoplasms: TRBC1-based flow cytometric assay experience

open access: yesTranslational Oncology, 2022
The identification of mature T cell neoplasms by flow cytometry is often challenging, due to overlapping features with reactive T cells and limitations of currently available T cell clonality assays. The description of an antibody specific for one of two
Manuela Capone   +14 more
doaj   +1 more source

Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

open access: yesCommunications Biology, 2021
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage   +6 more
doaj   +1 more source

An Early Myelosuppression in the Acute Mouse Sepsis Is Partly Outcome-Dependent

open access: yesFrontiers in Immunology, 2021
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response ...
Tomasz Skirecki   +6 more
doaj   +1 more source

A UV‐C LED‐based unit for continuous decontamination of the sheath fluid in a flow‐cytometric cell sorter

open access: yesEngineering in Life Sciences, 2022
Aseptic cell sorting is challenging, especially when a flow‐cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi.
Jenny Kirsch   +6 more
doaj   +1 more source

A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

open access: yesScientific Reports, 2021
In conventional flowcytometry one detector (primary) is dedicated for one fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover).
Debajit Bhowmick   +4 more
doaj   +1 more source

Cytokeratin and Vimentin Expression in Breast Cancer

open access: yesThe International Journal of Biological Markers, 2009
Background The transition from epithelial keratin to mesenchymal vimentin expression marks an important step in the malignant progression of breast cancer.
Hemangini H. Vora   +7 more
doaj   +1 more source

Detection of Central Nervous System Infiltration by Myeloid and Lymphoid Hematologic Neoplasms Using Flow Cytometry Analysis: Diagnostic Accuracy Study

open access: yesFrontiers in Medicine, 2018
IntroductionInfiltration of the central nervous system (CNS) by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal.
Laiz Cameirão Bento   +13 more
doaj   +1 more source

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