Results 31 to 40 of about 1,032,869 (292)

Automating flow cytometry [PDF]

Cytometry Part A, 2011
FLOW cytometry data analysis has been traditionally based on the identification of cell populations by using strategies founded on the definition of gates in bi-dimensional plots, where an experienced operator selects the subpopulation(s) of interest.
openaire   +2 more sources

Transient expansion of peripheral Lambda-expressing plasma cells represents a distinctive phenotype associated with SFTSV infection

Frontiers in Immunology
ObjectiveSevere fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high fatality rate. The mechanism of SFTS is widely recognized to be closely associated with dysregulated host immune responses.
Yawei Tang, Jingxue Wu, Yao Tian, Jiao Wang, Jianwei Liu, Qingyan Chang, Jie Zhu   +6 more
doaj   +1 more source

From Cellular Characteristics to Disease Diagnosis: Uncovering Phenotypes with Supercells [PDF]

, 2013
Cell heterogeneity and the inherent complexity due to the interplay of multiple molecular processes within the cell pose difficult challenges for current single-cell biology.
Banavar, Jayanth R., Biancotto, Angélique, Candia, Julian Marcelo, Cao, Kan, Dagur, Pradeep, Driscoll, Meghan, Losert, Wolfgang, Maritan, Amos, Maunu, Ryan, McCoy Jr., J Philip, Nida Sen, H., Nussenblatt, Robert B, Wei, Lai   +12 more
core   +5 more sources

Flow Cytometry Bioinformatics

PLoS Computational Biology, 2013
Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational ...
Kieran O'Neill, Nima Aghaeepour, Josef Spidlen, Ryan Brinkman   +3 more
openaire   +4 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Cell sorting based on pulse shapes from angle resolved detection of scattered light

Communications Biology
Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest.
Daniel Kage, Andrej Eirich, Kerstin Heinrich, Jenny Kirsch, Jan Popien, Alexander Wolf, Konrad v. Volkmann, Hyun-Dong Chang, Toralf Kaiser   +8 more
doaj   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Targeting interleukin-1β protects from aortic aneurysms induced by disrupted transforming growth factor β signaling [PDF]

, 2017
Aortic aneurysms are life-threatening conditions with effective treatments mainly limited to emergency surgery or trans-arterial endovascular stent grafts, thus calling for the identification of specific molecular targets.
Bizzotto, Dario, Braghetta, Paola, Bressan, Giorgio M., Carnevale, Daniela, Carnevale, Lorenzo, Carnevale, Raimondo, Casaburo, Manuel, Cifelli, Giuseppe, DA ROS, Francesco, Fardella, Stefania, Iacobucci, Roberta, Lembo, Giuseppe, Perrotta, Marialuisa, Vinciguerra, Iolanda   +13 more
core   +1 more source

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source