Results 21 to 30 of about 3,125,910 (203)

Cell sorting based on pulse shapes from angle resolved detection of scattered light

Communications Biology
Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest.
Daniel Kage, Andrej Eirich, Kerstin Heinrich, Jenny Kirsch, Jan Popien, Alexander Wolf, Konrad v. Volkmann, Hyun-Dong Chang, Toralf Kaiser   +8 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Using digital RNA counting and flow cytometry to compare mRNA with protein expression in acute leukemias. [PDF]

, 2012
BACKGROUND: The diagnosis of malignant hematologic diseases has become increasingly complex during the last decade. It is based on the interpretation of results from different laboratory analyses, which range from microscopy to gene expression profiling.
Alicia Rovo, Spertini, Olivier, Leda Leoncini (121167), Docquier, M., Olivier Spertini, Lovey Pierre-Yves, D'Asaro, Mathilde, Mathilde D’Asaro (121171), Sylvie Ruault-Jungblut (121169), Patrick Descombes (16839), Quarroz Stephane, Stephane Quarroz, Leoncini, Leda, Thomas Matthes, Rovo Alicia, Ruault-Jungblut, Sylvie, Paula Fernandez (88487), Olivier Schaad (16838), Ruault-Jungblut, S., Matthes Thomas, Max Solenthaler (121157), Schaad Olivier, Quarroz, S., Max Solenthaler, Spertini, O., Solenthaler Max, Fernandez, P., Olivier Schaad, Alicia Rovo (121163), Pierre-Yves Lovey, Fernandez Paula, Lovey, Pierre-Yves, Docquier Mylène, Mylène Docquier (105372), Leoncini Leda, Matthes, Thomas, Lovey, P.Y., Matthes, T., Pierre-Yves Lovey (121165), Solenthaler, Max, Thomas Matthes (121174), Descombes, P., Patrick Descombes, Descombes Patrick, Fernandez, Paula, Olivier Spertini (83526), D'Asaro, M., Rovo, A., Mathilde D'Asaro, Descombes, Patrick, Docquier, Mylene, Stephane Quarroz (121160), Leoncini, L., Schaad, Olivier, Swiss Cytometry Society, Schaad, O., Rovo, Alicia, Leda Leoncini, Spertini Olivier, Solenthaler, M., Ruault-Jungblut Sylvie, D’Asaro Mathilde, Sylvie Ruault-Jungblut, Quarroz, Stephane, Paula Fernandez, Mylène Docquier   +65 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Flow diagnostics essential code: a simple and brief format for the summary of leukemia phenotyping.

, 2014
BACKGROUND: Flow cytometry is a valuable part in the routine diagnostics of acute leukemia (AL). Although internationally recognized definitions of main AL subsets are available, there is currently no consensus format for the short summary of clinical
BULDINI, BARBARA, Kalina T, Dworzak MN, Mejstříková E, Hrušák O, Karawajew L, BASSO, GIUSEPPE, AIEOP BFM Flow Network, Ratei R, Bourquin JP, Rozenthal E, Gaipa G, Luria D, Sartor M   +13 more
core   +1 more source

HPLC and flow cytometry combined approach for HbF analysis in fetomaternal haemorrhage evaluation

Practical Laboratory Medicine
Introduction: Recently, a flow cytometric (FC) based test has been developed for detection of circulating fetal cells to replace the less accurate and reproducible Kleihauer-Betke test.FC test is easier to perform, it can distinguish the origin of fetal ...
Benedetta Peruzzi, Serena Guerrieri, Tiziana Biagioli, Luisa Lanzilao, Sara Pratesi, Sara Bencini, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Martina Tonelli, Marco Brogi, Manuela Capone, Alessio Mazzoni, Anna Maria Grazia Gelli, Alessandra Fanelli, Roberto Caporale, Francesco Annunziato   +16 more
doaj   +1 more source

Subcutaneous immunotherapy induces alterations in monocytes and dendritic cells homeostasis in allergic rhinitis patients

Allergy, Asthma & Clinical Immunology, 2018
Background Specific subcutaneous immunotherapy (SCIT) can achieve long-term remission in patients with allergic rhinitis (AR) through complex and still unknown mechanisms.
Letícia Sousa, Carmen Martín-Sierra, Celso Pereira, Graça Loureiro, Beatriz Tavares, Susana Pedreiro, António Martinho, Artur Paiva   +7 more
doaj   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

A computational framework to emulate the human perspective in flow cytometric data analysis [PDF]

, 2012
Background: In recent years, intense research efforts have focused on developing methods for automated flow cytometric data analysis. However, while designing such applications, little or no attention has been paid to the human perspective that is ...
Surajit Ray (83793), Pyne, S., Ray, S., Surajit Ray, Saumyadipta Pyne, Saumyadipta Pyne (9770)   +5 more
core   +2 more sources