Results 51 to 60 of about 359,525 (166)

spillR : spillover compensation in mass cytometry data

Bioinformatics, 2023
Abstract Motivation Channel interference in mass cytometry can cause spillover and may result in miscounting of protein markers. Chevrier et al. introduce an experimental and computational procedure to estimate and compensate for spillover implemented in their R package CATALYST.
Guazzini, Marco, Reisach, Alexander G, Weichwald, Sebastian, Seiler, Christof   +3 more
openaire   +4 more sources

Single-cell mass cytometry in immunological skin diseases

Frontiers in Immunology
Immune-related skin diseases represent a collective of dermatological disorders intricately linked to dysfunctional immune system processes. These conditions are primarily characterized by an immoderate activation of the immune system or deviant immune ...
Mingming Zhao, Mingming Zhao, Mingming Zhao, Mingming Zhao, Yuqi Cheng, Yuqi Cheng, Yuqi Cheng, Yuqi Cheng, Jinping Gao, Jinping Gao, Jinping Gao, Jinping Gao, Fusheng Zhou, Fusheng Zhou, Fusheng Zhou, Fusheng Zhou   +15 more
doaj   +1 more source

Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

STAR Protocols, 2021
Summary: This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in
Emma K. Jennings, David A.J. Lecky, Masahiro Ono, David Bending   +3 more
doaj   +1 more source

Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry

Bio-Protocol, 2015
Phosphorylation of tyrosine, serine, and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of kinases and phosphatases regulate intracellular protein phosphorylation in many different ...
Rosemary Fernandez, Holden Maecker
doaj   +1 more source

Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry

STAR Protocols, 2020
Summary: Assessing IL-2-induced phosopho-STAT5 (pSTAT5) content can reveal the cytokine responsiveness of individual T cells. Identifying distinct T cell subsets by nuclear transcription factors, such as Foxp3, and concurrently quantifying intracellular ...
Can Li, Jung-Hyun Park
doaj   +1 more source

Mass cytometry provides unprecedented insight into the role of B cells during the pathogenesis of multiple sclerosis

Advances in Clinical Neuroscience & Rehabilitation, 2020
Marsh-Wakefield, Felix, Byrne, Scott N, Hawke, Simon, Grau, Georges   +3 more
doaj   +1 more source

Singlet gating in mass cytometry [PDF]

Cytometry Part A, 2017
Liyun, Lai, Joo Guan, Yeo, Salvatore, Albani   +2 more
openaire   +2 more sources

Methods for analyzing tellurium imaging mass cytometry data.

PLoS ONE, 2019
Imaging mass cytometry (IMC) is a technique allowing visualization and quantification of over 40 biological parameters in a single experiment with subcellular spatial resolution, however most IMC experiments are limited to endpoint analysis with ...
Jay Bassan, Mark Nitz
doaj   +1 more source

Towards antibody-based immunotherapy development for platinum-resistant high-grade serous ovarian cancer

Scientific Reports
Epithelial ovarian cancer is the sixth most common malignant neoplasm in women and the leading cause of death from gynecological malignancies in the Western World.
Zivile Giedraityte, Noora Aarnio, Faez Amokrane Nait Mohamed, Line Bjørge, Seppo Meri   +4 more
doaj   +1 more source

Mass Cytometry—A Tool for the Curious: Networking in Berlin [PDF]

Cytometry Part A, 2020
Marie Burns, Axel R. Schulz, Désirée Kunkel, Manfred Hönig, Sarah Warth, Bertram Bengsch, Tyler Burns, Julia Reinhardt, Andreas Grützkau, Marie‐Laure Yaspo, Jan Sodenkamp, Ute Hoffmann, Henrik E. Mei   +12 more
openaire   +4 more sources