Results 51 to 60 of about 348,965 (301)

Imaging Mass Cytometry Raw Image Files-Multiple Myeloma

, 2023
Imaging mass cytometry data of multiple myeloma bone marrow and peripheral blood malignant plasma, immune, and other tumor microenvironment cells.
Maryam Setayesh (16813788)
core   +1 more source

FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses

STAR Protocols, 2023
Summary: Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Camilla Tiezzi, Marzia Rossi, Andrea Vecchi, Sara Doselli, Amalia Penna, Paola Fisicaro, Ilaria Montali, Camilla Ceccatelli Berti, Valentina Reverberi, Anna Montali, Gabriele Missale, Carlo Ferrari, Carolina Boni   +12 more
doaj   +1 more source

Compensation of signal spillover in suspension and imaging mass cytometry

, 2018
Raw mass cytometry and imaging mass cytometry data used to develop the compensation tool available within the R package ...
Chevrier, S (via Mendeley Data)
core   +1 more source

FACS-assisted CRISPR-Cas9 genome editing of human induced pluripotent stem cells

STAR Protocols, 2022
Summary: This manuscript proposes an efficient and reproducible protocol for the generation of genetically modified human induced pluripotent stem cells (hiPSCs) by genome editing using CRISPR-Cas9 technology.
Amandine Caillaud, Antoine Lévêque, Aurélie Thédrez, Aurore Girardeau, Robin Canac, Lise Bray, Manon Baudic, Julien Barc, Nathalie Gaborit, Guillaume Lamirault, Betty Gardie, Salam Idriss, Antoine Rimbert, Cédric Le May, Bertrand Cariou, Karim Si-Tayeb   +15 more
doaj   +1 more source

Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation

STAR Protocols, 2021
Summary: Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ’s immunity and function and are transferred during transplantation.
Amy Prosser, Sarah Dart, Irma Larma-Cornwall, Michaela Lucas   +3 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Mass cytometry reveals immune atlas of urothelial carcinoma

BMC Cancer, 2022
Immunotherapy has emerged as a robust clinical strategy for cancer treatment. PD1/PD-L1 inhibitors have been used as second-line therapy for urothelial carcinoma due to the high tumor mutational burden.
Qing Zhang, Wenlong Zhang, Tingsheng Lin, Wenfeng Lu, Xin He, Yuanzhen Ding, Wei Chen, Wenli Diao, Meng Ding, Pingping Shen, Hongqian Guo   +10 more
doaj   +1 more source

Applying imaging flow cytometry and immunofluorescence in studying the dynamic Golgi structure in cultured cells

STAR Protocols, 2022
Summary: The Golgi apparatus is subjected to fragmentation under several cellular processes such as mitosis. Here we describe two complementary approaches to analyze different Golgi morphological changes during its mitotic fragmentation, using classical ...
Inbal Wortzel, Ziv Porat, Rony Seger, Galia Maik-Rachline   +3 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Design Parameters for a Mass Cytometry Detectable HaloTag Ligand

, 2023
Mass cytometry permits the high dimensional analysis of complex biological samples; however, some techniques are not yet integrated into the mass cytometry workflow due to reagent availability.
Simon Latour (17564324), Edmond C. N. Wong (10783577), Mark Nitz (208005), Mitchell A. Winnik (1312674), Nicole Potter (17674000), Alison P. McGuigan (9592741), Hartland W. Jackson (8191659)   +6 more
core   +1 more source