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Ratiometric Barcoding for Mass Cytometry [PDF]
Analytical Chemistry, 2018 Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanide-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using 3 metal isotopes and 4 ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry ...
Xu Wu +6 more
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STAR Protocols, 2022 Summary: Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious.
Naoya Tatsumi +2 more
doaj +1 more source
STAR Protocols, 2022 Summary: Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure.
Leonie M. Weskamm +2 more
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Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
STAR Protocols, 2022 Summary: With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings.
Xiuxing Liu +4 more
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A Phospho-SIM in the Antiviral Protein PML is Required for Its Recruitment to HSV-1 Genomes
Cells, 2014 Herpes simplex virus type 1 (HSV-1) is a significant human pathogen that infects a large portion of the human population. Cells deploy a variety of defenses to limit the extent to which the virus can replicate.
Miles C. Smith +4 more
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Murine brain tumor microenvironment immunophenotyping using mass cytometry
STAR Protocols, 2022 Summary: Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model.
Brandon L. McClellan +4 more
doaj +1 more source
Sample Preparation for Mass Cytometry Analysis [PDF]
Journal of Visualized Experiments, 2017 Mass cytometry utilizes antibodies conjugated with heavy metal labels, an approach that has greatly increased the number of parameters and opportunities for deep analysis well beyond what is possible with conventional fluorescence-based flow cytometry. As with any new technology, there are critical steps that help ensure the reliable generation of high-
Ryan L, McCarthy +2 more
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reference code for mass cytometry data processing
, 2021 Code describing the key processing, clustering and classification steps starting from raw mass cytometry data from breast cancer patient derived ...
Callari Maurizio
core +1 more source
Scientific Reports, 2020 Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can
Yousef Risha +3 more
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MASS CYTOMETRY REVEALS A DISTINCT IMMUNOPHENOTYPE IN TELOMEROPATHIES
Hematology, Transfusion and Cell Therapy, 2023 Telomere-biology disorders (TBDs) are characterized by short telomeres and defective telomere repair, and clinical manifestations include bone marrow failure (BMF), myeloid neoplasms, fibrotic lung disease and liver cirrhosis.
W Gomes +7 more
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