Results 21 to 30 of about 359,525 (166)

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

Scientific Reports, 2020
Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can
Yousef Risha, Zoran Minic, Shahrokh M. Ghobadloo, Maxim V. Berezovski   +3 more
doaj   +1 more source

High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline

STAR Protocols, 2023
Summary: The current abundance of immunotherapy clinical trials presents an opportunity to learn about the underlying mechanisms and pharmacodynamic effects of novel drugs on the human immune system.
Guanning Wang, Olga Lyudovyk, Justin Y. Kim, Ya-Hui Lin, Yuval Elhanati, Divij Mathew, E. John Wherry, Ramin S. Herati, Allison R. Greenplate, Benjamin Greenbaum, Santosha A. Vardhana, Alexander C. Huang   +11 more
doaj   +1 more source

Evaluating cytokine production by flow cytometry using brefeldin A in mice

STAR Protocols, 2021
Summary: Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of ...
Stephen B. Kovacs, Changhoon Oh, Youssef Aachoui, Edward A. Miao   +3 more
doaj   +1 more source

Protocol for analyzing and visualizing antiviral immune responses after acute infection of the murine oral mucosa

STAR Protocols, 2021
Summary: The oral mucosa is an important site for virus infection and transmission, yet few animal models exist to examine the virology, pathology, and immunology of acute oral mucosal viral infection.
John P. Shannon, Christian R. Cherry, Sophia M. Vrba, Heather D. Hickman   +3 more
doaj   +1 more source

Extended live-cell barcoding approach for multiplexed mass cytometry

Scientific Reports, 2021
Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition.
Muharrem Muftuoglu, Li Li, Shaoheng Liang, Duncan Mak, Angelique J. Lin, Junxiang Fang, Jared K. Burks, Ken Chen, Michael Andreeff   +8 more
doaj   +1 more source

Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence

STAR Protocols, 2022
Summary: Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling.
Xiangyu Zhang, Jeremiah Stitham, Astrid Rodriguez-Velez, Se-Jin Jeong, Arick Park, Yu-Sheng Yeh, Divya Kapoor, Bettina Mittendorfer, Babak Razani   +8 more
doaj   +1 more source

Sample Preparation for Mass Cytometry Analysis [PDF]

Journal of Visualized Experiments, 2017
Mass cytometry utilizes antibodies conjugated with heavy metal labels, an approach that has greatly increased the number of parameters and opportunities for deep analysis well beyond what is possible with conventional fluorescence-based flow cytometry. As with any new technology, there are critical steps that help ensure the reliable generation of high-
Ryan L, McCarthy, Aundrietta D, Duncan, Michelle C, Barton   +2 more
openaire   +2 more sources

Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice

STAR Protocols, 2022
Summary: Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior ...
Michael R. D’Agostino, Sam Afkhami, Alisha Kang, Art Marzok, Matthew S. Miller, Zhou Xing   +5 more
doaj   +1 more source

High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM+ cells

STAR Protocols, 2022
Summary: Lymphocytes in the mouse small intestine (SI) epithelium are critical to establish effective barrier immunity. Here, we describe a magnetic cell separation protocol that employs anti-epithelial cellular adhesion molecule (EpCAM) antibodies to ...
Praveen Prakhar, Victoria Gonzalez, Jung-Hyun Park   +2 more
doaj   +1 more source

Mass Cytometry Imaging for the Study of Human Diseases—Applications and Data Analysis Strategies

Frontiers in Immunology, 2019
High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to ...
Heeva Baharlou, Heeva Baharlou, Nicolas P. Canete, Nicolas P. Canete, Anthony L. Cunningham, Anthony L. Cunningham, Andrew N. Harman, Andrew N. Harman, Ellis Patrick, Ellis Patrick   +9 more
doaj   +1 more source