Results 21 to 30 of about 3,665,969 (298)

Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry

STAR Protocols, 2022
Summary: Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious.
Naoya Tatsumi, Alejandro Davila-Pagan, Yosuke Kumamoto   +2 more
doaj   +1 more source

Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

STAR Protocols, 2022
Summary: Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure.
Leonie M. Weskamm, Christine Dahlke, Marylyn M. Addo   +2 more
doaj   +1 more source

Enabling Indium Channels for Mass Cytometry by Using Reinforced Cyclambased Chelating Polylysine [PDF]

, 2020
A metal containing polymer (MCP) based on a polylysine functionalized by In(III) chelates was synthesized. The chelator is based on a constrained dipicolinate cyclam that forms a highly inert In(III) complex.
Raphael, Tripier, Maryline, Beyler, Peng, Liu, Nick, Zabinyakov, olga, ornatsky, Taunia L. L., Closson, Laura, Grenier, Jothir Mayanantham, Pichaandi, Mitchell A., Winnik, Yefeng, Zhang, Alexandre, Bouzekri, Vladimir, Baranov   +11 more
core   +1 more source

Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations [PDF]

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Khatri, Indu, Alberto Orfao, Cristina Teodosio, Jacques J.M. van Dongen, Jager, Anniek L. de, Brigitta A.E. Naber, Inge F. de Laat, Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Hameetman, Marjolijn, Indu Khatri, Marjolijn Hameetman, Díez, Paula, Alesha Louis, Comans, Suzanne, Anniek L. de Jager, Paula Díez, Laat, Inge F. de, Pan, Kyra van der, Sara Kassem, Kyra van der Pan, Suzanne E.T. Comans, Naber, Brigitta A. E., Teodosio, Cristina   +25 more
core   +1 more source

Functional analysis of human circulating immune cells based on high-dimensional mass cytometry

STAR Protocols, 2022
Summary: With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings.
Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su   +4 more
doaj   +1 more source

A Phospho-SIM in the Antiviral Protein PML is Required for Its Recruitment to HSV-1 Genomes

Cells, 2014
Herpes simplex virus type 1 (HSV-1) is a significant human pathogen that infects a large portion of the human population. Cells deploy a variety of defenses to limit the extent to which the virus can replicate.
Miles C. Smith, Andrew C. Box, Jeffrey S. Haug, William S. Lane, David J. Davido   +4 more
doaj   +1 more source

Murine brain tumor microenvironment immunophenotyping using mass cytometry

STAR Protocols, 2022
Summary: Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model.
Brandon L. McClellan, Mahmoud S. Alghamri, Rohit Thalla, Pedro R. Lowenstein, Maria G. Castro   +4 more
doaj   +1 more source

Titrating Complex Mass Cytometry Panels [PDF]

Cytometry Part A, 2019
AbstractWe describe here a simple and efficient antibody titration approach for cell‐surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well‐characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE.
Gullaksen, Stein-Erik, Bader, Lucius, Hellesøy, Monica, Sulen, Andre, Fagerholt, Oda Helen Eck, Engen, Caroline Benedicte Nitter, Skavland, Jørn, Gjertsen, Bjørn Tore, Gavasso, Sonia   +8 more
openaire   +4 more sources

High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline

STAR Protocols, 2023
Summary: The current abundance of immunotherapy clinical trials presents an opportunity to learn about the underlying mechanisms and pharmacodynamic effects of novel drugs on the human immune system.
Guanning Wang, Olga Lyudovyk, Justin Y. Kim, Ya-Hui Lin, Yuval Elhanati, Divij Mathew, E. John Wherry, Ramin S. Herati, Allison R. Greenplate, Benjamin Greenbaum, Santosha A. Vardhana, Alexander C. Huang   +11 more
doaj   +1 more source

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

Scientific Reports, 2020
Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can
Yousef Risha, Zoran Minic, Shahrokh M. Ghobadloo, Maxim V. Berezovski   +3 more
doaj   +1 more source