Results 11 to 20 of about 348,965 (301)

Platelet surface marker analysis by mass cytometry

Platelets, 2020
Mass cytometry is a next generation flow cytometry technology which analyzes cells one at a time (up to 1000/sec) using mass spectrometry to detect probes labeled with rare-earth metals.
Thomas A. Blair, Andrew L. Frelinger
doaj   +2 more sources

Titrating Complex Mass Cytometry Panels [PDF]

Cytometry Part A, 2019
AbstractWe describe here a simple and efficient antibody titration approach for cell‐surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well‐characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE.
Gullaksen, Stein-Erik, Bader, Lucius, Hellesøy, Monica, Sulen, Andre, Fagerholt, Oda Helen Eck, Engen, Caroline Benedicte Nitter, Skavland, Jørn, Gjertsen, Bjørn Tore, Gavasso, Sonia   +8 more
openaire   +6 more sources

Mass Cytometry: Single Cells, Many Features [PDF]

Cell, 2016
Technology development in biological research often aims to either increase the number of cellular features that can be surveyed simultaneously or enhance the resolution at which such observations are possible. For decades, flow cytometry has balanced these goals to fill a critical need by enabling the measurement of multiple features in single cells ...
Spitzer, Matthew H, Nolan, Garry P
openaire   +6 more sources

Single-cell phenotypes of peripheral blood immune cells in early and late stages of non-alcoholic fatty liver disease [PDF]

Clinical and Molecular Hepatology, 2023
Background/Aims Immune and inflammatory cells respond to multiple pathological hits in the development of nonalcoholic steatohepatitis (NASH) and fibrosis.
Kathryn Jane Waller, Hajar Saihi, Wenhao Li, James Hallimond Brindley, Anja De Jong, Wing-kin Syn, Conrad Bessant, William Alazawi   +7 more
doaj   +1 more source

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

STAR Protocols, 2023
Summary: Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4+ T cell differentiation in the intestine.
Jinzhi Duan, Yanan Sun, Juan D. Matute, Richard S. Blumberg   +3 more
doaj   +1 more source

Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease

STAR Protocols, 2022
Summary: Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune
Calvin Krollmann, Kevin Cieslak, Ruth-Miriam Koerber, Hella Luksch, Angela Rösen-Wolff, Peter Brossart, Lino L. Teichmann   +6 more
doaj   +1 more source

Flow Cytometry II: Mass and Imaging Cytometry [PDF]

Journal of Investigative Dermatology, 2015
Correction to: Journal of Investigative Dermatology (2015) 135, e36; doi:10.1038/id.2015 ...
Doan, Hung, Chinn, Garrett M., Jahan-Tigh, Richard R.   +2 more
openaire   +3 more sources

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

STAR Protocols, 2021
Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types ...
M. Sadman Sakib, Godwin Sokpor, Huu Phuc Nguyen, Andre Fischer, Tran Tuoc   +4 more
doaj   +1 more source

Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages

STAR Protocols, 2023
Summary: Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins.
Ignazio Antignano, Lily Keane, Melania Capasso   +2 more
doaj   +1 more source

Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

STAR Protocols, 2021
Summary: Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor.
Simon Wabitsch, Justin D. McCallen, Chi Ma, Tim F. Greten   +3 more
doaj   +1 more source