Results 11 to 20 of about 3,665,969 (298)

Platelet surface marker analysis by mass cytometry

Platelets, 2020
Mass cytometry is a next generation flow cytometry technology which analyzes cells one at a time (up to 1000/sec) using mass spectrometry to detect probes labeled with rare-earth metals.
Thomas A. Blair, Andrew L. Frelinger
doaj   +2 more sources

Flow cytometry in the analysis of cellular populations [PDF]

, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Flow cytometry has become a valuable tool in cell biology. By analyzing large number of cells individually using light-scatter and fluorescence measurements, this technique reveals both
Castaño Boldú, Esther, Comas Riu, Jaume F.   +1 more
core   +6 more sources

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

STAR Protocols, 2023
Summary: Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4+ T cell differentiation in the intestine.
Jinzhi Duan, Yanan Sun, Juan D. Matute, Richard S. Blumberg   +3 more
doaj   +1 more source

Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease

STAR Protocols, 2022
Summary: Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune
Calvin Krollmann, Kevin Cieslak, Ruth-Miriam Koerber, Hella Luksch, Angela Rösen-Wolff, Peter Brossart, Lino L. Teichmann   +6 more
doaj   +1 more source

Flow Cytometry II: Mass and Imaging Cytometry [PDF]

Journal of Investigative Dermatology, 2015
Correction to: Journal of Investigative Dermatology (2015) 135, e36; doi:10.1038/id.2015 ...
Doan, Hung, Chinn, Garrett M., Jahan-Tigh, Richard R.   +2 more
openaire   +3 more sources

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

STAR Protocols, 2021
Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types ...
M. Sadman Sakib, Godwin Sokpor, Huu Phuc Nguyen, Andre Fischer, Tran Tuoc   +4 more
doaj   +1 more source

DataSheet_1_Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations.docx [PDF]

, 2023
IntroductionMonitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care.
Kassem, Sara, Kyra van der Pan (13796077), Khatri, Indu, Brigitta A.E. Naber (15649463), Paula Díez (10052081), Indu Khatri (511338), Jager, Anniek L. de, Suzanne E.T. Comans (15649466), Louis, Alesha, Orfao, Alberto, Dongen, J. J. M. van, Jacques J.M. van Dongen (10153076), Alesha Louis (13796104), Inge F. de Laat (13796089), Alberto Orfao (108164), Hameetman, Marjolijn, Díez, Paula, Cristina Teodosio (143750), Sara Kassem (13796098), Comans, Suzanne, Laat, Inge F. de, Anniek L. de Jager (13796101), Pan, Kyra van der, Naber, Brigitta A. E., Marjolijn Hameetman (6177341), Teodosio, Cristina   +25 more
core   +1 more source

Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages

STAR Protocols, 2023
Summary: Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins.
Ignazio Antignano, Lily Keane, Melania Capasso   +2 more
doaj   +1 more source

Ratiometric Barcoding for Mass Cytometry [PDF]

Analytical Chemistry, 2018
Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanide-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using 3 metal isotopes and 4 ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry ...
Xu Wu, Quinn DeGottardi, I-Che Wu, Li Wu, Jiangbo Yu, William W. Kwok, Daniel T. Chiu   +6 more
openaire   +2 more sources

Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

STAR Protocols, 2021
Summary: Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor.
Simon Wabitsch, Justin D. McCallen, Chi Ma, Tim F. Greten   +3 more
doaj   +1 more source