Results 11 to 20 of about 359,525 (166)

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

STAR Protocols, 2021
Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types ...
M. Sadman Sakib, Godwin Sokpor, Huu Phuc Nguyen, Andre Fischer, Tran Tuoc   +4 more
doaj   +1 more source

Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages

STAR Protocols, 2023
Summary: Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins.
Ignazio Antignano, Lily Keane, Melania Capasso   +2 more
doaj   +1 more source

Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

STAR Protocols, 2021
Summary: Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor.
Simon Wabitsch, Justin D. McCallen, Chi Ma, Tim F. Greten   +3 more
doaj   +1 more source

Ratiometric Barcoding for Mass Cytometry [PDF]

Analytical Chemistry, 2018
Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanide-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using 3 metal isotopes and 4 ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry ...
Xu Wu, Quinn DeGottardi, I-Che Wu, Li Wu, Jiangbo Yu, William W. Kwok, Daniel T. Chiu   +6 more
openaire   +2 more sources

Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry

STAR Protocols, 2022
Summary: Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious.
Naoya Tatsumi, Alejandro Davila-Pagan, Yosuke Kumamoto   +2 more
doaj   +1 more source

Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

STAR Protocols, 2022
Summary: Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure.
Leonie M. Weskamm, Christine Dahlke, Marylyn M. Addo   +2 more
doaj   +1 more source

Functional analysis of human circulating immune cells based on high-dimensional mass cytometry

STAR Protocols, 2022
Summary: With the advantages of high resolution and high dimension, mass cytometry is implemented to analyze the blood complex immune system in clinical settings.
Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su   +4 more
doaj   +1 more source

A Phospho-SIM in the Antiviral Protein PML is Required for Its Recruitment to HSV-1 Genomes

Cells, 2014
Herpes simplex virus type 1 (HSV-1) is a significant human pathogen that infects a large portion of the human population. Cells deploy a variety of defenses to limit the extent to which the virus can replicate.
Miles C. Smith, Andrew C. Box, Jeffrey S. Haug, William S. Lane, David J. Davido   +4 more
doaj   +1 more source

Murine brain tumor microenvironment immunophenotyping using mass cytometry

STAR Protocols, 2022
Summary: Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model.
Brandon L. McClellan, Mahmoud S. Alghamri, Rohit Thalla, Pedro R. Lowenstein, Maria G. Castro   +4 more
doaj   +1 more source

Titrating Complex Mass Cytometry Panels [PDF]

Cytometry Part A, 2019
AbstractWe describe here a simple and efficient antibody titration approach for cell‐surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well‐characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE.
Gullaksen, Stein-Erik, Bader, Lucius, Hellesøy, Monica, Sulen, Andre, Fagerholt, Oda Helen Eck, Engen, Caroline Benedicte Nitter, Skavland, Jørn, Gjertsen, Bjørn Tore, Gavasso, Sonia   +8 more
openaire   +4 more sources