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Hematology/Oncology Clinics of North America, 2018
Multiparameter flow cytometry (MFC) is valuable in the diagnosis and monitoring of most hematological malignancies. Although the assessment of cellular infiltrates in Waldenström macroglobulinemia (WM) relies on morphology and immunohistochemistry grounds, there is evidence pointing to the clinical significance of MFC in this disease.
Amaia, Gascue +2 more
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Multiparameter flow cytometry (MFC) is valuable in the diagnosis and monitoring of most hematological malignancies. Although the assessment of cellular infiltrates in Waldenström macroglobulinemia (WM) relies on morphology and immunohistochemistry grounds, there is evidence pointing to the clinical significance of MFC in this disease.
Amaia, Gascue +2 more
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Science, 2018
Seeing ghosts In fluorescence-activated cell sorting, characteristic target features are labeled with a specific fluorophore, and cells displaying different fluorophores are sorted. Ota et al. describe a technique called ghost cytometry that allows cell sorting based on the morphology of the cytoplasm ...
Sadao Ota +11 more
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Seeing ghosts In fluorescence-activated cell sorting, characteristic target features are labeled with a specific fluorophore, and cells displaying different fluorophores are sorted. Ota et al. describe a technique called ghost cytometry that allows cell sorting based on the morphology of the cytoplasm ...
Sadao Ota +11 more
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2001
Abstract Single cell biochemistry, single cell screening, and assessment of heterogeneity in cell populations have become feasible in both fundamental and industrial situations. These strategies have benefited from the following happy marriage.
Coba De La Pena, Teodoro, Brown, Spencer
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Abstract Single cell biochemistry, single cell screening, and assessment of heterogeneity in cell populations have become feasible in both fundamental and industrial situations. These strategies have benefited from the following happy marriage.
Coba De La Pena, Teodoro, Brown, Spencer
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2003
Flow cytometry technique is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antrbodies or ligands (e.g., cytokines, hormones) that bind specific cell-assocrated molecules. Flow cytometry is quantitative, fast (analysis of >l0,000 cells/min), reliable, and reproducible.
M G, Ormerod, P R, Imrie
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Flow cytometry technique is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antrbodies or ligands (e.g., cytokines, hormones) that bind specific cell-assocrated molecules. Flow cytometry is quantitative, fast (analysis of >l0,000 cells/min), reliable, and reproducible.
M G, Ormerod, P R, Imrie
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Current Opinion in Chemical Biology, 2003
Chemical cytometry refers to the use of high-sensitivity analytical tools to characterize single cells. These tools include mass spectrometry, electrochemistry and capillary separation methods. This review focuses on the use of capillary electrophoresis coupled with high-sensitivity detection to characterize single cells.
Norman J, Dovichi, Shen, Hu
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Chemical cytometry refers to the use of high-sensitivity analytical tools to characterize single cells. These tools include mass spectrometry, electrochemistry and capillary separation methods. This review focuses on the use of capillary electrophoresis coupled with high-sensitivity detection to characterize single cells.
Norman J, Dovichi, Shen, Hu
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2013
Hyperspectral cytometry is an emerging technology for single-cell analysis that combines ultrafast optical spectroscopy and flow cytometry. Spectral cytometry systems utilize diffraction gratings or prism-based monochromators to disperse fluorescence signals from multiple labels (organic dyes, nanoparticles, or fluorescent proteins) present in each ...
Gérald, Grégori +6 more
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Hyperspectral cytometry is an emerging technology for single-cell analysis that combines ultrafast optical spectroscopy and flow cytometry. Spectral cytometry systems utilize diffraction gratings or prism-based monochromators to disperse fluorescence signals from multiple labels (organic dyes, nanoparticles, or fluorescent proteins) present in each ...
Gérald, Grégori +6 more
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2016
Flow cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, using an optical-to-electronic coupling system. A wide range of applications are discussed for this tool, ranging from traditional immunology to monitoring of cell transfection and/or screening of protein ...
V. Tirino +3 more
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Flow cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, using an optical-to-electronic coupling system. A wide range of applications are discussed for this tool, ranging from traditional immunology to monitoring of cell transfection and/or screening of protein ...
V. Tirino +3 more
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Current Protocols in Cell Biology, 2003
AbstractCommon methods applicable to flow cytometry make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell morphology and chromatin condensation, which occur during apoptosis, can be detected by analysis with ...
Zbigniew Darzynkiewicz +2 more
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AbstractCommon methods applicable to flow cytometry make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell morphology and chromatin condensation, which occur during apoptosis, can be detected by analysis with ...
Zbigniew Darzynkiewicz +2 more
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Overview of Flow Cytometry and Fluorescent Probes for Cytometry
1994Abstract This chapter provides an introduction to the use of fluorescent probes in flow cytometry. Sample preparation for the use of surface labeling with antibodies as well as for the use of nucleic acid probes is discussed. The utility of cell sorting is also discussed.
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Advancing Cytometry for Immunology
European Journal of Immunology, 2012Brief SummaryCytometry is a key technology for immunology. It allows researchers to scrutinize the cells of the immune system in molecular detail, and to assess phenotype and function at the level of individual cells, no matter how rare these cells may be.
Andreas Radbruch +5 more
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