Results 181 to 190 of about 29,630 (208)

Stepwise Protocol for Cytospin-enhanced Smearing for Scraped Corneal Epithelial Cells

Applied Immunohistochemistry & Molecular Morphology, 2016
Proteins and antigens present on the cell surface are usually determined by immunofluorescence staining. Uniform distribution of cells is required to appreciate the presence of surface proteins. Improper smearing or crushing of the corneal epithelial cells can potentially destroy the cellular integrity.
Mani V, Jeyalatha, Jambulingam, Malathi, Hajib N, Madhavan   +2 more
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Evidence‐based adequacy criteria for instrumented urine cytology using cytospin preparations

Diagnostic Cytopathology, 2018
Cytospin preparations of instrumented urine cytology specimens with less than 10 urothelial cells or more than 50 urothelial cells/10 hpfs are both associated with significantly increased false negative rates compared to cases with 10‐49 urothelial cells/10 hpfs.
Andrew A. Renshaw, Edwin W. Gould
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A Cytospin Technique for Spreading Plant Metaphases Suitable for Immunofluorescence Studies

Biotechnic & Histochemistry, 1998
Recent immunofluorescence techniques enable the localization of various cellular antigens, thus providing a powerful tool for cell and molecular biology research. Serious problems occur, however, when these techniques are applied to plant material. The presence of the cellulose wall can be a barrier to reproducible penetration of antibodies into cells ...
R, Hladilová, J, Siroký, B, Vyskot
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Sequential identification of apoptotic and necrotic cells on individual cytospin preparations

Journal of Immunological Methods, 1998
Here, we describe a simple methodology which allows the consecutive differentiation of apoptotic and necrotic cells on the same cytospin preparation of fresh and cultured cells. In this methodology, necrotic cells are initially identified on the cytospin preparations using trypan blue followed by the identification of in situ DNA fragmentation using ...
P, Pantelidis, G, Santilli, R A, Knight
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Automated Immunocytochemistry for Cellular Markers on Cell Smears and Cytospins

Journal of Histotechnology, 1992
Abstract A rapid, automated immunocytochemical staining method for the Instrumentation Laboratory Code-On stainer was developed to detect cellular markers in cell smears and cytospins prepared from fine needle aspirates of solid tumors and from body fluids collected by brushing, scraping, or washing.
David W. Cowan, Debra B. Novotny, Susan J. Maygarden, Katherine B. Pryzwansky   +3 more
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Improvement cell blocks with EBUS-TBNA by Cytospin rinse

Interventional Pulmonology, 2017
Introduction: Real-time endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a recently introduced method of biopsy of the mediastinal lymph nodes. This is a safe and minimally invasive and save procedure. The material can be obtained as a smear or cell block (CB).
Jan Siwiec, Marek Michnar, Barbara Mackiewicz, Juliusz Pankowski, Janusz Milanowski   +4 more
openaire   +1 more source

Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats

Journal of the American Animal Hospital Association, 2008
Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease.
Dehard, Sandrine, Bernaerts, Frederique, Peeters, Dominique, Detilleux, Johann, Mc Entee, Kathleen, Day, Michael J, Clercx, Cécile   +6 more
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Morphological Analysis of Cell Death by Cytospinning Followed by Rapid Staining

Cold Spring Harbor Protocols, 2016
Identifying and characterizing different forms of cell death can be facilitated by staining internal cellular structures with dyes such as hematoxylin and eosin (H&E). These dyes stain the nucleus and cytoplasm, respectively, and optimized reagents (e.g., Rapi-Diff, Rapid Stain, or Quick Dip) are commonly used in pathology laboratories.
Crowley, Lisa C., Marfell, Brooke J., Waterhouse, Nigel J.   +2 more
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Preparation of Cells for Microscopy using Cytospin

2013
Microscopy is a simple, direct technique for examining the morphology of cells and their organelles. Cells are immobilized on a solid support that is optically suitable for microscopy, and then fixed. When coupled with antibody-based immunofluorescent or immunocytochemical methods, the expression of specific proteins can be quantified and localized ...
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Identification of Adenocarcinoma in Cytospin Preparations of Effusions Using Monoclonal Antibody B72.3

American Journal of Clinical Pathology, 1986
Metastatic adenocarcinoma in human effusions can be difficult to distinguish from reactive mesothelial cells, particularly if the malignant cells are rare, have bland cytologic features, or occur as single cells rather than clusters of cells. Monoclonal antibody (MAb) B72.3, generated against a membrane-enriched fraction of breast carcinoma, has been ...
S E, Martin, S, Moshiri, A, Thor, V, Vilasi, E W, Chu, J, Schlom   +5 more
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