Results 181 to 190 of about 20,743 (215)

Leukostat Staining of Cytospin Preparations to Detect Apoptosis

Cold Spring Harbor Protocols, 2006
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel and Douglas R. Green This protocol was adapted from “Apoptosis Assays,” Chapter 15, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998.
Shailaja, Kasibhatla, Gustavo P, Amarante-Mendes, Deborah, Finucane, Thomas, Brunner, Ella, Bossy-Wetzel, Douglas R, Green   +5 more
openaire   +3 more sources

Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats

Journal of the American Animal Hospital Association, 2008
Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease.
Dehard, Sandrine, Bernaerts, Frederique, Peeters, Dominique, Detilleux, Johann, Mc Entee, Kathleen, Day, Michael J, Clercx, Cécile   +6 more
openaire   +4 more sources

Morphological Analysis of Cell Death by Cytospinning Followed by Rapid Staining

Cold Spring Harbor Protocols, 2016
Identifying and characterizing different forms of cell death can be facilitated by staining internal cellular structures with dyes such as hematoxylin and eosin (H&E). These dyes stain the nucleus and cytoplasm, respectively, and optimized reagents (e.g., Rapi-Diff, Rapid Stain, or Quick Dip) are commonly used in pathology laboratories.
Crowley, Lisa C., Marfell, Brooke J., Waterhouse, Nigel J.   +2 more
openaire   +4 more sources

The use of cytospin monolayer technique in the cytological diagnosis of vulval and anal disease

Cytopathology, 2001
The use of cytospin monolayer technique in the cytological diagnosis of vulval and anal disease This pilot study investigated the use of the non‐invasive cytospin monolayer technique in the diagnosis and screening of neoplastic and non‐neoplastic vulval disease.
J Crow, A B Maclean
exaly   +3 more sources

Sequential identification of apoptotic and necrotic cells on individual cytospin preparations

Journal of Immunological Methods, 1998
Here, we describe a simple methodology which allows the consecutive differentiation of apoptotic and necrotic cells on the same cytospin preparation of fresh and cultured cells. In this methodology, necrotic cells are initially identified on the cytospin preparations using trypan blue followed by the identification of in situ DNA fragmentation using ...
P, Pantelidis, G, Santilli, R A, Knight
openaire   +2 more sources

Automated Immunocytochemistry for Cellular Markers on Cell Smears and Cytospins

Journal of Histotechnology, 1992
Abstract A rapid, automated immunocytochemical staining method for the Instrumentation Laboratory Code-On stainer was developed to detect cellular markers in cell smears and cytospins prepared from fine needle aspirates of solid tumors and from body fluids collected by brushing, scraping, or washing.
David W. Cowan, Debra B. Novotny, Susan J. Maygarden, Katherine B. Pryzwansky   +3 more
openaire   +2 more sources

A Cytospin Technique for Spreading Plant Metaphases Suitable for Immunofluorescence Studies

Biotechnic & Histochemistry, 1998
Recent immunofluorescence techniques enable the localization of various cellular antigens, thus providing a powerful tool for cell and molecular biology research. Serious problems occur, however, when these techniques are applied to plant material. The presence of the cellulose wall can be a barrier to reproducible penetration of antibodies into cells ...
R, Hladilová, J, Siroký, B, Vyskot
openaire   +2 more sources

Improvement cell blocks with EBUS-TBNA by Cytospin rinse

Interventional Pulmonology, 2017
Introduction: Real-time endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a recently introduced method of biopsy of the mediastinal lymph nodes. This is a safe and minimally invasive and save procedure. The material can be obtained as a smear or cell block (CB).
Jan Siwiec, Marek Michnar, Barbara Mackiewicz, Juliusz Pankowski, Janusz Milanowski   +4 more
openaire   +1 more source

Stepwise Protocol for Cytospin-enhanced Smearing for Scraped Corneal Epithelial Cells

Applied Immunohistochemistry & Molecular Morphology, 2016
Proteins and antigens present on the cell surface are usually determined by immunofluorescence staining. Uniform distribution of cells is required to appreciate the presence of surface proteins. Improper smearing or crushing of the corneal epithelial cells can potentially destroy the cellular integrity.
Mani V, Jeyalatha, Jambulingam, Malathi, Hajib N, Madhavan   +2 more
openaire   +2 more sources

A Basis for Distinguishing Cultured Dendritic Cells and Macrophages in Cytospins and Fixed Sections

Pediatric and Developmental Pathology, 2005
There is a burgeoning literature on the contrasting role of intratumoral dendritic cells (DCs) and tumor-associated macrophages, making reliable identification of both cell types in clinical and experimental tissue sections important. However, because these cell types are closely related and share several differentiation antigens, their absolute ...
Jukka, Vakkila, Michael T, Lotze, Connie, Riga, Ronald, Jaffe   +3 more
openaire   +2 more sources