Results 141 to 150 of about 1,797,149 (300)

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

A daily temperature rhythm in the human brain predicts survival after brain injury. [PDF]

Brain, 2022
Rzechorzek NM, Thrippleton MJ, Chappell FM, Mair G, Ercole A, Cabeleira M, CENTER-TBI High Resolution ICU (HR ICU) Sub-Study Participants and Investigators, Rhodes J, Marshall I, O'Neill JS.   +9 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Daily Temperature Effect on Seedling Growth Dynamic of Three Invasive Alien Species. [PDF]

Front Plant Sci, 2022
Pepe M, Gratani L, Crescente MF, Puglielli G, Varone L.   +4 more
europepmc   +1 more source

Historical global and regional spatiotemporal patterns in daily temperature

Frontiers in Environmental Science
The abrupt increase in surface air temperature over the last few decades has received abundant scholarly and popular attention. However, less attention has focused on the specific nature of the warming spatially and seasonally, using high-resolution ...
Md Adilur Rahim, Md Adilur Rahim, Md Adilur Rahim, Robert V. Rohli, Robert V. Rohli, Rubayet Bin Mostafiz, Rubayet Bin Mostafiz, Rubayet Bin Mostafiz, Nazla Bushra, Nazla Bushra, Carol J. Friedland, Carol J. Friedland   +11 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Light triggers a network switch between circadian morning and evening oscillators controlling behaviour during daily temperature cycles. [PDF]

PLoS Genet, 2022
Lorber C, Leleux S, Stanewsky R, Lamaze A.   +3 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Acclimation Effects of Natural Daily Temperature Variation on Longevity, Fecundity, and Thermal Tolerance of the Diamondback Moth (Plutella xylostella). [PDF]

Insects, 2022
Xing K, Zhao F.
europepmc   +1 more source