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Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion [PDF]

open access: yesMembranes, 2023
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban   +5 more
doaj   +4 more sources

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]

open access: yesMembranes
Giant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić   +2 more
doaj   +4 more sources

Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions [PDF]

open access: yesMembranes
Giant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method.
Ivan Mardešić   +2 more
doaj   +5 more sources

Preparation of giant unilamellar vesicles from damp lipid film for better lipid compositional uniformity

open access: yesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2012
Giant unilamellar vesicles (GUVs) containing cholesterol often have a wide distribution in lipid composition. In this study, GUVs of 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC)/1,2-distearoyl-sn-glycero-3-phosphocholine(DSPC)/cholesterol and 1,2-diphytanoyl-sn-glycero-3-phosphocholine(diPhyPC)/1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC ...
Baykal-Caglar, Eda   +3 more
openaire   +2 more sources

Glyburide inhibits the Cryopyrin/Nalp3 inflammasome [PDF]

open access: yes, 2009
Inflammasomes activate caspase-1 for processing and secretion of the cytokines interleukin-1 beta (IL-1 beta) and IL-18. Cryopyrin/NALP3/NLRP3 is an essential component of inflammasomes triggered by microbial ligands, danger-associated molecular patterns
Mohamed Lamkanfi   +43 more
core   +2 more sources

Mechanism of cellular uptake of genotoxic silica nanoparticles. [PDF]

open access: yes, 2012
Mechanisms for cellular uptake of nanoparticles have important implications for nanoparticulate drug delivery and toxicity. We have explored the mechanism of uptake of amorphous silica nanoparticles of 14 nm diameter, which agglomerate in culture medium ...
Brown, AP   +5 more
core   +3 more sources

Adsorption and binding dynamics of graphene-supported phospholipid membranes using the QCM-D technique [PDF]

open access: yes, 2018
We report on the adsorption dynamics of phospholipid membranes on graphene-coated substrates using the quartz crystal microbalance with dissipation monitoring (QCM-D) technique.
Goodwin, S.   +5 more
core   +2 more sources

The watering of tall trees - Embolization and recovery [PDF]

open access: yes, 2015
We can propound a thermo-mechanical understanding of the ascent of sap to the top of tall trees thanks to a comparison between experiments associated with the cohesion-tension theory and the disjoining pressure concept for liquid thin-films.
Gouin, Henri
core   +6 more sources

Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells [PDF]

open access: yes, 1985
cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+
Lazarides, Elias   +3 more
core   +3 more sources

Single beam grating coupled interferometry: high resolution miniaturized label-free sensor for plate based parallel screening [PDF]

open access: yes, 2012
Grating Coupled Interferometry (GCI) using high quality waveguides with two incoupling and one outcoupling grating areas is introduced to increase and precisely control the sensing length of the device; and to make the sensor design suitable for plate ...
Cottier, Kaspar   +3 more
core   +1 more source

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