Results 1 to 10 of about 1,487 (146)

Optimization of Giant Unilamellar Vesicle Electroformation for Phosphatidylcholine/Sphingomyelin/Cholesterol Ternary Mixtures [PDF]

open access: yesMembranes, 2022
Artificial vesicles are important tools in membrane research because they enable studying membrane properties in controlled conditions. Giant unilamellar vesicles (GUVs) are specially interesting due to their similarity in size to eukaryotic cells.
Marija Raguž   +2 more
exaly   +4 more sources

Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results

open access: yesMembranes, 2021
Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Marija Raguž   +2 more
exaly   +3 more sources

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion [PDF]

open access: yesMembranes, 2023
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban   +5 more
doaj   +2 more sources

Frequency-Dependent Electroformation of Giant Unilamellar Vesicles in 3D and 2D Microelectrode Systems

open access: yesMicromachines, 2017
A giant unilamellar vesicle (GUV), with similar properties to cellular membrane, has been widely studied. Electroformation with its simplicity and accessibility has become the most common method for GUV production.
Jun Yang, Ning Hu, Ning Hu
exaly   +3 more sources

Electroformation of Giant Vesicles on a Polymer Mesh

open access: yesMembranes, 2011
Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs) from egg yolk phosphatidylcholine under applied electric voltage was examined on a substrate of a polymer mesh placed between two planar indium tin oxide coated glass electrodes.
Yukihisa Okumura
exaly   +3 more sources

Electroformation of Giant Vesicles on Indium Tin Oxide (ITO)-Coated Poly(ethylene terephthalate) (PET) Electrodes

open access: yesMembranes, 2011
Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs) from egg yolk phosphatidylcholine was examined using a poly(ethylene terephthalate) sheet coated with indium tin oxide (ITO-PET) as the electrode material.
Yukihisa Okumura
exaly   +3 more sources

Effect of Counter Electrode in Electroformation of Giant Vesicles

open access: yesMembranes, 2011
Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs), from egg yolk phosphatidylcholine, was examined varying the shape of the counter electrode.
Yukihisa Okumura
exaly   +3 more sources

A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles

open access: yesBio-Protocol, 2022
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen   +1 more
doaj   +1 more source

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter

open access: yesScientific Reports, 2021
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
Patricia P. M. Mathiassen   +2 more
doaj   +1 more source

Comparative Studies of the Electrochemical Behavior of Silver Electrode in Chloride, Bromide and Iodide Aqueous Solutions

open access: yesInternational Journal of Electrochemical Science, 2010
Comparative studies of the electrochemical behaviour of Ag in aqueous NaCl, NaBr and NaI solutions of various concentrations ranging from 0.1 M to 1.0 M have been made by means of cyclic voltammetry and chronoamperometry techniques, complemented with SEM
Hamdy H. Hassan   +3 more
doaj   +1 more source

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