Membranes, 2021 Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Marija Raguz +2 more
exaly +3 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions [PDF]
MembranesGiant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method.
Ivan Mardešić +2 more
doaj +2 more sources
Instant Membrane Stabilization by Damage‐Triggered Actin Polymerization in Giant Unilamellar Vesicles [PDF]
ChemBioChem, Volume 27, Issue 6, 27 March 2026.Damage‐triggered actin polymerization stabilizes membrane pores in giant unilamellar vesicles (GUVs). Membrane poration drives divalent cation influx, inducing rapid F‐actin assembly at the defect site. The resulting actin plug mechanically arrests pore expansion and preserves vesicle morphology, recapitulating the “plugging” phase of single‐cell ...
Huong Thanh Nguyen +3 more
wiley +2 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion [PDF]
Membranes, 2023 Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban +5 more
doaj +2 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
doaj +2 more sources
Membranes, 2022 Artificial vesicles are important tools in membrane research because they enable studying membrane properties in controlled conditions. Giant unilamellar vesicles (GUVs) are specially interesting due to their similarity in size to eukaryotic cells.
Marija Raguz +2 more
exaly +3 more sources
Membranes, 2011 Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs) from egg yolk phosphatidylcholine was examined using a poly(ethylene terephthalate) sheet coated with indium tin oxide (ITO-PET) as the electrode material.
Yukihisa Okumura
exaly +3 more sources
Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
doaj +2 more sources
The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model [PDF]
JDS CommunicationsGiant unilamellar vesicle (GUV) bilayers were constructed from polar lipids and cholesterol by electroformation as a model system to investigate the formation of ordered lipid domains (OLD) within the milk fat globule membrane (MFGM).
Haotian Zheng +2 more
doaj +2 more sources
Developing giant plasma membrane vesicles from Leishmania cells to investigate the role of membrane proteins in photodynamic inactivation [PDF]
Photochemistry and Photobiology, Volume 102, Issue 2, Page 480-488, March/April 2026.In this study, we developed giant plasma membrane vesicles (GPMVs) from Leishmania amazonensis promastigote membranes to investigate the role of protein‐embedded membranes in methylene blue (MB) photooxidation. Upon MB photoactivation in model lipid membranes (giant unilamellar vesicles, GUVs), a distinct photooxidation effect was observed, marked by ...
Maressa D. F. de Souza +3 more
wiley +2 more sources