Reconstitution and functional studies of hamster P-glycoprotein in giant liposomes [PDF]
PLoS ONE, 2018 This paper describes the preparation of giant unilamellar vesicles with reconstituted hamster P-glycoprotein (Pgp, ABCB1) for studying the transport activity of this efflux pump in individual liposomes using optical microscopy.
Soohyun Park, Sheereen Majd
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Microscopic Observation of Membrane Fusion between Giant Liposomes and Baculovirus Budded Viruses Activated by the Release of a Caged Proton [PDF]
Membranes, 2023 Baculovirus (Autographa californica multiple nucleopolyhedrovirus, AcMNPV) is an envelope virus possessing a fusogenic protein, GP64, which can be activated under weak acidic conditions close to those in endosomes.
Misako Nishigami +2 more
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Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries [PDF]
Micromachines, 2021 The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating ...
Saúl C. Gómez +6 more
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Mechanisms of Self-Assembly of Giant Unilamellar Vesicles in the Army Liposome Formulation (ALF) Family of Vaccine Adjuvants [PDF]
PharmaceuticsBackground/Objectives: Army Liposome Formulation with QS21 (ALFQ) is a vaccine adjuvant formulation consisting of liposomes that contain saturated zwitterionic and anionic phospholipids, 55 mol% cholesterol, and small molar amounts of monophosphoryl ...
Calin Nicolescu +4 more
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Collapsin Response Mediator Protein 2 (CRMP2) Modulates Induction of the Mitochondrial Permeability Transition Pore in a Knock-In Mouse Model of Alzheimer’s Disease [PDF]
CellsHyperphosphorylated collapsin response mediator protein 2 (CRMP2) is elevated in the cerebral cortex of an APP-SAA knock-in mouse model of Alzheimer’s disease and binds the adenine nucleotide translocase (ANT) in a phosphorylation-dependent manner.
Tatiana Brustovetsky +2 more
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Facile and versatile PDMS-glass capillary double emulsion formation device coupled with rapid purification toward microfluidic giant liposome generation [PDF]
Microsystems & NanoengineeringThe exceptional ability of liposomes to mimic a cellular lipid membrane makes them invaluable tools in biomembrane studies and bottom-up synthetic biology.
Mostafa Bakouei +6 more
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Giant liposomes in physiological buffer using electroformation in a flow chamber
Biochimica Et Biophysica Acta - Biomembranes, 2005 We describe a method to obtain giant liposomes (diameter 10-100 microm) in solutions of high ionic strength to perform a membrane-binding assay under physiological conditions. Using electroformation on ITO electrodes, we formed surface-attached giant liposomes in solutions of glycerol in a flow chamber and then introduced solutions of high ionic ...
Michael Mayer, Daniel J Estes
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Nano-liposomes: Historical Perspectives and Major Advances in Cancer Therapy. A critical Review
British Journal of Pharmacy, 2023 Since the discovery of liposomes by Bangham and co-workers in 1960, giant strides in the field of liposomes as drug carriers for cancer therapy have been achieved. From the very first generation of liposomes known as conventional liposomes to the stealth
Ibilola Mary Cardoso-Daodu +2 more
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Application of Various Types of Liposomes in Drug Delivery Systems [PDF]
Advanced Pharmaceutical Bulletin, 2017 Liposomes, due to their various forms, require further exploration. These structures can deliver both hydrophilic and hydrophobic drugs for cancer, antibacterial, antifungal, immunomodulation, diagnostics, ophtalmica, vaccines, enzymes and genetic ...
Mehran Alavi +2 more
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Materials Research Express, 2021 A methodology which provides a high efficiency of giant vesicle formation was established using the gentle hydration method and a microplotter equipment.
Juan R Avendaño-Gomez +3 more
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