Microscopic Observation of Membrane Fusion between Giant Liposomes and Baculovirus Budded Viruses Activated by the Release of a Caged Proton [PDF]
Membranes, 2023 Baculovirus (Autographa californica multiple nucleopolyhedrovirus, AcMNPV) is an envelope virus possessing a fusogenic protein, GP64, which can be activated under weak acidic conditions close to those in endosomes.
Misako Nishigami +2 more
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Postsynthetic Photocontrol of Giant Liposomes via Fusion-Based Photolipid Doping. [PDF]
Langmuir, 2022 We report on photolipid doping of giant unilamellar vesicles (GUVs) via vesicle fusion with small unilamellar photolipid vesicles (pSUVs), which enables retroactive optical control of the membrane properties. We observe that vesicle fusion is light-dependent, if the phospholipids are neutral.
Pritzl SD +5 more
europepmc +7 more sources
Reconstitution and functional studies of hamster P-glycoprotein in giant liposomes. [PDF]
PLoS ONE, 2018 This paper describes the preparation of giant unilamellar vesicles with reconstituted hamster P-glycoprotein (Pgp, ABCB1) for studying the transport activity of this efflux pump in individual liposomes using optical microscopy.
SooHyun Park, Sheereen Majd
doaj +3 more sources
Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries [PDF]
Micromachines, 2021 The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating ...
Saúl C. Gómez +6 more
doaj +3 more sources
Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes. [PDF]
J Membr Biol, 2017 To quantitatively examine the effect of membrane organization on lateral diffusion, we studied fluorescent carbocyanine lipid analogues and EGFP-tagged, single-pass transmembrane proteins in systems of decreasing complexity: (i) the plasma membrane (PM ...
Worch R +3 more
europepmc +2 more sources
Electroformation of giant unilamellar vesicles from large liposomes [PDF]
The European Physical Journal Special TopicsAbstractGiant unilamellar vesicles (GUVs) are widely used as model systems for biological membranes to study membrane-related processes in a precisely controlled in vitro environment, owing to their biophysical properties. The classical technique for the formation of giant liposomes starts with the dissolution of lipids or lipid mixtures in an organic ...
Huriye D. Uzun +4 more
openaire +4 more sources
Mechanisms of Self-Assembly of Giant Unilamellar Vesicles in the Army Liposome Formulation (ALF) Family of Vaccine Adjuvants [PDF]
PharmaceuticsBackground/Objectives: Army Liposome Formulation with QS21 (ALFQ) is a vaccine adjuvant formulation consisting of liposomes that contain saturated zwitterionic and anionic phospholipids, 55 mol% cholesterol, and small molar amounts of monophosphoryl ...
Calin Nicolescu +4 more
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On-Chip Inverted Emulsion Method for Fast Giant Vesicle Production, Handling, and Analysis
Micromachines, 2020 Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Naresh Yandrapalli +2 more
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Facile and versatile PDMS-glass capillary double emulsion formation device coupled with rapid purification toward microfluidic giant liposome generation [PDF]
Microsystems & NanoengineeringThe exceptional ability of liposomes to mimic a cellular lipid membrane makes them invaluable tools in biomembrane studies and bottom-up synthetic biology.
Mostafa Bakouei +6 more
doaj +2 more sources
Collapsin Response Mediator Protein 2 (CRMP2) Modulates Induction of the Mitochondrial Permeability Transition Pore in a Knock-In Mouse Model of Alzheimer’s Disease [PDF]
CellsHyperphosphorylated collapsin response mediator protein 2 (CRMP2) is elevated in the cerebral cortex of an APP-SAA knock-in mouse model of Alzheimer’s disease and binds the adenine nucleotide translocase (ANT) in a phosphorylation-dependent manner.
Tatiana Brustovetsky +2 more
doaj +2 more sources