Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese +6 more
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Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu +2 more
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A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets [PDF]
Membranes, 2023 Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
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Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
PLoS ONE, 2023 Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud +3 more
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Microfluidic production, stability and loading of synthetic giant unilamellar vesicles [PDF]
Scientific ReportsIn advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles ...
Mart Ernits +11 more
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Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics
Communications Chemistry, 2021 The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli +3 more
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Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles. [PDF]
Nano Lett, 2023 Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens, or the therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic.
Azadbakht A +4 more
europepmc +3 more sources
Thermal fluctuations of the lipid membrane determine particle uptake into Giant Unilamellar Vesicles. [PDF]
Nat Commun, 2023 Particulates, bacteria and viruses wrap into cell membranes. Here the authors use optical tweezers, particle tracking and mathematical modelling to show that the uptake process into giant vesicles is influenced by thermal membrane fluctuations ...
Ayala YA +3 more
europepmc +2 more sources
Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields. [PDF]
Adv Phys X, 2023 Knowledge of the material properties of membranes is crucial to understanding cell viability and physiology. A number of methods have been developed to probe membranes in vitro, utilizing the response of minimal biomimetic membrane models to an external ...
Aleksanyan M +5 more
europepmc +2 more sources
A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +2 more sources