Giant unilamellar vesicles as a model system for studying ion transport. [PDF]
Biophys RevFletcher M +3 more
europepmc +2 more sources
Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]
PLoS ONE, 2012 Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn +3 more
doaj +1 more source
Low-cost equipment for electroformation of Giant Unilamellar Vesicles [PDF]
HardwareX, 2018 Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium
Javier Alejandro Bellon +2 more
openalex +3 more sources
Asymmetric Giant Unilamellar Vesicles [PDF]
Biophysical Journal, 2010 The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Hu, Peichi C., Malmstadt, Noah
openaire +1 more source
Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining
Bio-Protocol, 2023 Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum +2 more
doaj +1 more source
A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets. [PDF]
Membranes (Basel), 2023 Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Zhang Y, Obuchi H, Toyota T.
europepmc +2 more sources
Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]
Biophysical Journal, 2013 Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas +8 more
openaire +3 more sources
Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics
Communications Chemistry, 2021 The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli +3 more
doaj +1 more source
Functional reconstitution of plant plasma membrane H<sup>+</sup>-ATPase into giant unilamellar vesicles. [PDF]
Sci RepMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Uzun HD +3 more
europepmc +2 more sources