Results 31 to 40 of about 347,263 (150)

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network

open access: yesBMC Bioinformatics, 2022
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee   +4 more
doaj   +1 more source

Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture

open access: yesCommunications Biology, 2021
By encapsulating proteins in giant unilamellar vesicles, Bashirzadeh et al find that actin crosslinkers, α-actinin and fascin, can self-assemble with actin into complex structures that depend on the degree of confinement.
Yashar Bashirzadeh   +9 more
doaj   +1 more source

Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ ions: A small-angle neutron scattering study [PDF]

open access: yes, 2007
Dipalmitoylphosphatidylcholine (DPPC) was hydrated in 0.2-60 mM solution of CaCl 2 in heavy water and thoroughly homogenized by freezing-thawing process.
Lengyel, Adrián   +3 more
core   +1 more source

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

open access: yesBio-Protocol, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris   +2 more
doaj   +1 more source

Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]

open access: yesPLoS ONE, 2015
Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda   +4 more
doaj   +1 more source

Heat-induced formation of single giant unilamellar vesicles [PDF]

open access: yes, 2011
Giant unilamellar vesicles (GUVs) are an excellent model system for the investigation of lipid membranes, the study of membrane proteins and ion channels in a biomimetic environment, and in the creation of artificial cells.
Aldo Jesorka   +18 more
core   +1 more source

Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)

open access: yesScientific Reports, 2021
Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira   +9 more
doaj   +1 more source

A Note on Vestigial Osmotic Pressure

open access: yesMembranes, 2023
Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like ...
Hao Wu   +2 more
doaj   +1 more source

Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]

open access: yesAngew Chem Int Ed Engl, 2021
Success in the bottom-up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase-separated giant unilamellar lipid vesicles (GUVs).
Dreher Y   +4 more
europepmc   +2 more sources

Membrane sculpting by curved DNA origami scaffolds

open access: yesNature Communications, 2018
BAR domain proteins feature a “banana-like” shape which is thought to aid membrane scaffolding and membrane tubulation. Here authors use DNA origami mimicking BAR domains, giant unilamellar vesicles and fluorescence imaging to study how different BAR ...
Henri G. Franquelim   +4 more
doaj   +1 more source

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