Results 31 to 40 of about 19,171 (192)

Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]

open access: yesPLoS ONE, 2012
Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn   +3 more
doaj   +1 more source

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

open access: yesBio-Protocol, 2023
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum   +2 more
doaj   +1 more source

Asymmetric Giant Unilamellar Vesicles [PDF]

open access: yesBiophysical Journal, 2010
The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Hu, Peichi C., Malmstadt, Noah
openaire   +1 more source

Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles

open access: yesbioRxiv, 2021
Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in reconstitution experiments. They are also widely used in research on synthetic cells as they provide a mechanically responsive reaction compartment that allows for ...
Lori van de Cauter   +9 more
semanticscholar   +1 more source

Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]

open access: yesBiophysical Journal, 2013
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas   +8 more
openaire   +3 more sources

Antimicrobial peptide magainin 2-induced rupture of single giant unilamellar vesicles comprising E. coli polar lipids.

open access: yesBiochimica et Biophysica Acta - Biomembranes, 2022
Most antimicrobial peptides (AMPs) damage the cell membrane of bacterial cells and induce rapid leakage of the internal cell contents, which is a main cause of their bactericidal activity.
M. Billah   +3 more
semanticscholar   +1 more source

Chemical communication at the synthetic cell/living cell interface

open access: yesCommunications Chemistry, 2021
Synthetic models of cells are becoming increasingly sophisticated, but engineering communication between these and living cells remains challenging. Here the authors review modes of communication and signal processing between living cells and synthetic ...
Vincent Mukwaya   +2 more
doaj   +1 more source

Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

open access: yesNature Communications, 2021
Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E.
Kevin Jahnke   +11 more
doaj   +1 more source

Shear stress induced lipid order and permeability changes of giant unilamellar vesicles.

open access: yesBiochimica et Biophysica Acta - General Subjects, 2022
BACKGROUND The permeability of a lipid bilayer is a function of its phase state and depends non-linearly on thermodynamic variables such as temperature, pressure or pH.
Nicolas Färber   +3 more
semanticscholar   +1 more source

Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening

open access: yesCHIMIA, 2022
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj   +1 more source

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