Results 31 to 40 of about 26,375 (226)

Instant Membrane Stabilization by Damage-Triggered Actin Polymerization in Giant Unilamellar Vesicles. [PDF]

Chembiochem
Damage‐triggered actin polymerization stabilizes membrane pores in giant unilamellar vesicles (GUVs). Membrane poration drives divalent cation influx, inducing rapid F‐actin assembly at the defect site. The resulting actin plug mechanically arrests pore expansion and preserves vesicle morphology, recapitulating the “plugging” phase of single‐cell ...
Nguyen HT, Lee SH, Kim CH, Shin K.
europepmc   +2 more sources

Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles. [PDF]

ACS Synth Biol, 2023
One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist to generate giant unilamellar vesicle-based artificial cells.
Supramaniam P, Wang Z, Chatzimichail S, Parperis C, Kumar A, Ho V, Ces O, Salehi-Reyhani A.   +7 more
europepmc   +5 more sources

Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]

PLoS ONE, 2012
Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn, Eva Boenzli, Peter Eggenberger Hotz, Martin M Hanczyc   +3 more
doaj   +1 more source

Asymmetric Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2010
The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Hu, Peichi C., Malmstadt, Noah
openaire   +1 more source

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

Bio-Protocol, 2023
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum, Huriye Uzun, Thomas Günther Pomorski   +2 more
doaj   +1 more source

Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles

bioRxiv, 2021
Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in reconstitution experiments. They are also widely used in research on synthetic cells as they provide a mechanically responsive reaction compartment that allows for ...
Lori van de Cauter, F. Fanalista, Lennard van Buren, Nicola de Franceschi, Elisa Godino, Sharon Bouw, C. Danelon, C. Dekker, G. Koenderink, Kristina A. Ganzinger   +9 more
semanticscholar   +1 more source

Antimicrobial peptide magainin 2-induced rupture of single giant unilamellar vesicles comprising E. coli polar lipids.

Biochimica et Biophysica Acta - Biomembranes, 2022
Most antimicrobial peptides (AMPs) damage the cell membrane of bacterial cells and induce rapid leakage of the internal cell contents, which is a main cause of their bactericidal activity.
M. Billah, M. Rashid, Marzuk Ahmed, M. Yamazaki   +3 more
semanticscholar   +1 more source

Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2013
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas, Tsai, Feng-Ching, Koenderink, Gijsje H., Schmidt, Thais F., Itri, Rosângela, Meier, Wolfgang, Schmatko, Tatiana, Schröder, André, Marques, Carlos   +8 more
openaire   +3 more sources

Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles

ACS Synthetic Biology, 2023
Spontaneous and induced front-rear polarization and a subsequent asymmetric actin cytoskeleton is a crucial event leading to cell migration, a key process involved in a variety of physiological and pathological conditions such as tissue development ...
Andreas Fink, C. R. Doll, Ana Yagüe Relimpio, Yannik Dreher, Joachim P. Spatz, Kerstin Göpfrich, E. A. Cavalcanti‐Adam   +6 more
semanticscholar   +1 more source

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]

, 2013
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh, AH Stegh, AJ Garcia-Saez, AJ Garcia-Saez, AM Chinnaiyan, Ana-Jesus García-Sáez, B Apellaniz, B Leber, Beate Klösgen, Boris Zhivotovsky, C Scaffidi, D Ardail, D Ardail, D Chandra, D Marsh, D Marsh, D Needham, DW Chang, E Evans, E Krieghoff, E van den Brink-van der Laan, EE Varfolomeev, Eyal Gottlieb, F Gonzalvez, F Gonzalvez, F Gonzalvez, F Gonzalvez, FC Kischkel, François Gonzalvez, G Kroemer, GC Shearman, H Zhang, HR Stennicke, J Zha, Jesus Ayala-Sanmartin, JF Lovell, JH Li, JP Medema, K Milovic-Holm, KH Lee, KM Boatright, L Mathivet, Liberty François-Moutal, M Lamblet, M Lutter, M Lutter, M Muzio, M Muzio, MA Hughes, Mark Perry, MI Angelova, MO Hengartner, MP Boldin, N Keller, Olivier Jalmar, P Juo, P Ratanabanangkoon, Patrice X. Petit, Petra Schwille, PJ Jost, RF Epand, S Zhang, T Granjon, T Kuwana, T Parasassi, Thierry Granjon, VA Torres, VA Torres, W Helfrich, W Rawicz, WC Yeh, X Luo, Y Zaltsman, ZT Schug   +73 more
core   +9 more sources