Results 11 to 20 of about 6,262 (195)
Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles. [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-A (E to Z) and blue (Z to E) light.
Albanese P +6 more
europepmc +9 more sources
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 AbstractIn the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic compartmentalisation ability and their resemblance in size and shape to eukaryotic cells.
Jorik Waeterschoot +2 more
exaly +5 more sources
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles. [PDF]
ACS OmegaWe demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion.
Dhanawat G, Dey M, Singh A, Parveen N.
europepmc +6 more sources
Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles. [PDF]
Membranes (Basel)The plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions. Over the last decade several methods have been developed that allow for the fabrication of large or giant unilamellar vesicles ...
McDonough J +5 more
europepmc +5 more sources
Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
PLoS One, 2023 Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive technique that utilizes precisely controlled electric field pulses of micro- to millisecond durations to
Wadud MA +3 more
europepmc +5 more sources
Thermodynamic Relaxation Drives Expulsion in Giant Unilamellar Vesicles [PDF]
Physical Biology, 2013 We investigated the thermodynamic relaxation of giant unilamellar vesicles (GUVs) which contained small vesicles within their interior. Quenching these vesicles from their fluid phase (T>Tm) through the phase transition in the gel state ...
Christian T. Leirer +3 more
openalex +6 more sources
Bulk Self-Assembly of Giant, Unilamellar Vesicles
ACS Nano, 2020 The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive. In bulk solution, membrane-forming molecules such as phospholipids, single-tailed surfactants, and block ...
James T Kindt +2 more
exaly +5 more sources
Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]
Angew Chem Int Ed Engl, 2021 AbstractSuccess in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs). We derive an analytical model based on the vesicle geometry, which makes four quantitative predictions that we verify
Dreher Y +4 more
europepmc +7 more sources
Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes [PDF]
ACS Omega, 2017 Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles.
Valerio Pereno +6 more
openaire +8 more sources
Visualization and Quantification of Transmembrane Ion Transport into Giant Unilamellar Vesicles [PDF]
Angewandte Chemie International Edition, 2014 AbstractTransmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are ...
Hennie Valkenier +3 more
openalex +8 more sources