Bulk Self-Assembly of Giant, Unilamellar Vesicles. [PDF]
ACS Nano, 2020 The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive.
Kindt JT, Szostak JW, Wang A.
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Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]
Angew Chem Int Ed Engl, 2021 Success in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs).
Dreher Y +4 more
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Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]
MembranesThe plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough +5 more
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Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles. [PDF]
J Vis Exp, 2021 Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes and thus are a great tool to study membrane-related cellular processes in vitro.
Bashirzadeh Y +4 more
europepmc +5 more sources
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
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Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
Scientific Reports, 2021 Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira +9 more
doaj +2 more sources
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles [PDF]
ACS OmegaWe demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion.
Garvita Dhanawat +3 more
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Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]
PLoS ONE, 2015 Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda +4 more
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Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network [PDF]
BMC Bioinformatics, 2022 Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee +4 more
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Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening
CHIMIA, 2022 Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj +5 more sources