Bulk Self-Assembly of Giant, Unilamellar Vesicles [PDF]
ACS Nano, 2020 The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive.
Anna Wang, James Kindt, Jack W Szostak
exaly +6 more sources
Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]
Angew Chem Int Ed Engl, 2021 Success in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs).
Dreher Y +4 more
europepmc +8 more sources
Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]
MembranesThe plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough +5 more
doaj +4 more sources
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
doaj +4 more sources
Reconstitution of Active Plant H+-ATPase AHA2 in Giant Unilamellar Vesicles [PDF]
Bio-ProtocolMembrane transporters mediate the selective movement of ions and molecules across biological membranes and are essential for cellular homeostasis. However, their functional characterization in living cells is often complicated by the complexity of the ...
Huriye Uzun, Thomas Pomorski
doaj +2 more sources
3D DNA origami pincers that multitask on giant unilamellar vesicles. [PDF]
Sci AdvProteins self-assemble to function in living cells. They may execute essential tasks in the form of monomers, complexes, or supramolecular cages via oligomerization, achieving a sophisticated balance between structural topology and functional dynamics ...
Zhan P +7 more
europepmc +2 more sources
A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +2 more sources
Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields. [PDF]
Adv Phys X, 2023 Knowledge of the material properties of membranes is crucial to understanding cell viability and physiology. A number of methods have been developed to probe membranes in vitro, utilizing the response of minimal biomimetic membrane models to an external ...
Aleksanyan M +5 more
europepmc +2 more sources
Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles. [PDF]
J Vis Exp, 2021 Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes and thus are a great tool to study membrane-related cellular processes in vitro.
Bashirzadeh Y +4 more
europepmc +2 more sources
Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
Scientific Reports, 2021 Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira +9 more
doaj +2 more sources