Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles. [PDF]
Nano Lett, 2023 Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens or the diagnosis and therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic. In this letter, we use an experimental model system based on Giant Unilamellar Vesicles (GUVs) and dumbbell-shaped ...
Azadbakht A +4 more
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Forming and loading giant unilamellar vesicles with acoustic jetting
Biomicrofluidics, 2020 Giant unilamellar vesicles (GUVs) are a useful platform for reconstituting and studying membrane-bound biological systems, offering reduced complexity compared to living cells. Several techniques exist to form GUVs and populate them with biomolecules of interest.
Maxim Armstrong +3 more
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Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer. [PDF]
Open Res EurRoberti E +3 more
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Reconstitution of Active Plant H<sup>+</sup>-ATPase AHA2 in Giant Unilamellar Vesicles. [PDF]
Bio ProtocMembrane transporters mediate the selective movement of ions and molecules across biological membranes and are essential for cellular homeostasis. However, their functional characterization in living cells is often complicated by the complexity of the ...
Uzun HD, Pomorski TG.
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Induced phagocytic particle uptake into a giant unilamellar vesicle
Soft Matter, 2014 Phagocytosis, the uptake and ingestion of solid particles into living cells, is a central mechanism of our immune system. Due to the complexity of the uptake mechanism, the different forces involved in this process are only partly understood. Therefore the usage of a giant unilamellar vesicle (GUV) as the simplest biomimetic model for a cell allows one
Andreas Meinel +3 more
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Selective membrane wetting of phase-separated giant unilamellar vesicles by coacervate droplets. [PDF]
Nat CommunJoseph E +3 more
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A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles. [PDF]
Bio Protoc, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Mathiassen PPM, Pomorski TG.
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Curcumin-Induced Stabilization of Protein-Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles. [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Okagu OD, Abioye RO, Udenigwe CC.
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The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model. [PDF]
JDS CommunZheng H, Jiménez-Flores R, Everett DW.
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Heat-Induced Formation of Single Giant Unilamellar Vesicles [PDF]
Biophysical Journal, 2012 Céline Billerit
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