Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
doaj +2 more sources
Soft Matter, 2022 Giant unilamellar vesicles (GUVs) are micrometer-sized model membrane systems that can be viewed directly under the microscope. They serve as scaffolds for the bottom-up creation of synthetic cells, targeted drug delivery and have been used in many in ...
Dolder N, Müller P, von Ballmoos C.
europepmc +2 more sources
Rotaxanes with a photoresponsive macrocycle modulate the lipid bilayers of large and giant unilamellar vesicles [PDF]
Communications ChemistryRotaxanes equipped with actuators hold great potential for developing highly functional molecular machines. Such systems could significantly enhance our ability to study and manipulate biological and artificial membranes.
Udyogi N. K. Conthagamage +10 more
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Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles.
Vasil Georgiev +3 more
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ChemBioChem, 2019 In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's similarity to natural biomembranes, have been widely used as ...
Akanksha Moga +3 more
semanticscholar +3 more sources
Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels [PDF]
Bio-Protocol, 2020 Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris +2 more
doaj +4 more sources
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
doaj +2 more sources
Facile generation of giant unilamellar vesicles using polyacrylamide gels [PDF]
Scientific Reports, 2020 Giant unilamellar vesicles (GUVs) are model cell-sized systems that have broad applications including drug delivery, analysis of membrane biophysics, and synthetic reconstitution of cellular machineries.
Eric Parigoris +7 more
semanticscholar +6 more sources
DNA Origami Assembly Inside Liposomes via Nanopore‐Mediated DNA Transportation
Small StructuresAssembly of DNA origami nanostructures inside giant unilamellar vesicles (GUVs) is demonstrated by supplying approximately 200 unique staple strands from the external environment.
Shoji Iwabuchi +5 more
doaj +2 more sources
Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles.
Journal of Colloid and Interface Science, 2019 Among the potential novel therapeutics to treat bacterial infections, antimicrobial peptides (AMPs) are a very promising substitute due to their broad-spectrum activity and rapid bactericidal action.
A. Mescola +4 more
semanticscholar +3 more sources