Intravesicular Solute Delivery and Surface Area Regulation in Giant Unilamellar Vesicles Driven by Cycles of Osmotic Stresses. [PDF]
J Am Chem SocPhospholipid bilayers are dynamic cellular components that undergo constant changes in their topology, facilitating a broad diversity of physiological functions including endo- and exocytosis, cell division, and intracellular trafficking.
Sambre PD, Ho JCS, Parikh AN.
europepmc +2 more sources
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles [PDF]
ACS OmegaWe demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion.
Garvita Dhanawat +3 more
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ChemBioChem, 2019 In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's similarity to natural biomembranes, have been widely used as ...
Tom Robinson +2 more
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Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
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Rotaxanes with a photoresponsive macrocycle modulate the lipid bilayers of large and giant unilamellar vesicles [PDF]
Communications ChemistryRotaxanes equipped with actuators hold great potential for developing highly functional molecular machines. Such systems could significantly enhance our ability to study and manipulate biological and artificial membranes.
Udyogi N. K. Conthagamage +10 more
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Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles.
Vasil Georgiev +3 more
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Osmotic Gradients Induce Bio-Reminiscent Morphological Transformations in Giant Unilamellar Vesicles
Frontiers in Physiology, 2012 We report observations of large-scale, in-plane and out-of-plane membrane deformations in giant uni- and multilamellar vesicles composed of binary and ternary lipid mixtures in the presence of net transvesicular osmotic gradients.
Atul Parikh +2 more
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Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
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Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles
Journal of Colloid and Interface Science, 2019 Among the potential novel therapeutics to treat bacterial infections, antimicrobial peptides (AMPs) are a very promising substitute due to their broad-spectrum activity and rapid bactericidal action.
Andrea Mescola +2 more
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Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes [PDF]
ACS Omega, 2017 Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles.
Valerio Pereno +6 more
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